SARS-CoV-2 M Gene Tagged ORF (codon optimized)

CAT#: VC102559

  • TrueORF®

Myc-DDK-tagged ORF clone for SARS-CoV-2 Membrane Glycoprotein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724393. Note: ORF is codon optimized



  View other clones from "SARS-CoV-2" (41)

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CNY 2,400.00

CNY 3,800.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
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Specifications

Product Data
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>VC102559 representing NC_045512, codon optimized.
Due to the complexity of NC_045512, the ORF clone is codon optimized for mammalian Expression.
The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.

Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCCGACTCAAATGGGACAATCACCGTCGAAGAACTGAAGAAACTTCTGGAACAGTGGAACCTCGTT
ATCGGCTTTCTCTTTCTCACCTGGATCTGCTTGCTCCAGTTTGCATACGCCAACCGAAATCGATTTCTT
TATATCATCAAACTCATCTTCCTTTGGTTGCTCTGGCCCGTCACCCTCGCATGTTTCGTTTTGGCTGCA
GTGTATAGAATAAATTGGATTACCGGCGGAATTGCTATTGCTATGGCTTGTCTTGTGGGACTTATGTGG
CTCAGTTACTTTATTGCTTCCTTTAGATTGTTTGCCCGGACTCGCTCAATGTGGTCTTTTAACCCTGAA
ACTAACATCCTCCTTAACGTCCCCCTTCATGGCACAATCCTGACGCGACCCCTCCTCGAAAGTGAGCTG
GTGATCGGCGCTGTAATCCTGCGAGGCCATCTCAGAATCGCCGGGCACCATCTCGGCCGCTGCGATATA
AAAGACCTGCCCAAAGAAATTACAGTTGCAACGTCTCGCACCCTTTCCTATTATAAGTTGGGAGCTAGT
CAGAGAGTCGCAGGAGACTCTGGTTTTGCCGCATACTCTCGATACCGCATTGGAAACTACAAGCTTAAC
ACCGACCACTCATCATCTTCCGACAACATCGCCCTGCTCGTTCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
>VC102559 representing NC_045512
Blue=ORF Red=Cloning site Green=Tag(s)

MADSNGTITVEELKKLLEQWNLVIGFLFLTWICLLQFAYANRNRFLYIIKLIFLWLLWPVTLACFVLAA
VYRINWITGGIAIAMACLVGLMWLSYFIASFRLFARTRSMWSFNPETNILLNVPLHGTILTRPLLESEL
VIGAVILRGHLRIAGHHLGRCDIKDLPKEITVATSRTLSYYKLGASQRVAGDSGFAAYSRYRIGNYKLN
TDHSSSSDNIALLVQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NC_045512
ORF Size 666 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq YP_009724393
RefSeq ORF 666 bp
MW 25.1 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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