putative DNA-directed RNA polymerase II (NC_003494) Virus Tagged ORF Clone

CAT#: VC102324

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for putative DNA-directed RNA polymerase II [Infectious spleen and kidney necrosis virus], codon optimized for human cell expression, NP_612256



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CNY 15870.00


货期*
7周

规格
    • 10 ug

Product images

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Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC102324 represents NCBI reference of NP_612256 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGGCTCATTTGTCATATTGGACTGCACCCCAGCGGCTTGTTCACCACCACATCGCCCACTATGAAC
ATCTGCTCAGGGTTCAGATCCCTCAAGTGGTGGAAGGGGAGCCTCCTGTCATCATCCACAACCACTCCGT
AACATTTGGCGATGTAGCATACTCCAAGCCATCACCTGAGACCCCAGGGCACGCCCGAGCCTACGATGAG
ACTTATGAGACGACGGTTTCATGCAAAGCCTATCTGACCTGTATGGCCACTGGGGCAAAGCATGTGCAGC
ACATCCACAATCTCGCAAAAATGCCAGTGATGGTCAGAGCCCCTCCTCTCTGTCAGGGTATCGGCGAGTG
CGAAAAGGACCCTGGGGGTTATTTCATTGTGAAAGGCAAGGAGAGGATTATCATCCCTCACATCCACACA
GCCTACAACATTCCAATCACAATGCACTGTACCACCAAAAAGACCCTGGTTTGTGAAATGAGGACTCATA
ACGAGACCACCGGCAATAACCTGCTTGTTAAAGTGAGACTGATGCAAGGCTGTATCATCGAGATGAGCGT
GCCTTACATTAAGAGCTTTCTGCCAGCCGGTGCTGTGTTCAATGCCCTGGGCGTGAGCGAAGAAGACATG
TTGAGATATTGCAATCTGGCGTGGCAGCGGACGACGCAAGGGGGCTACGTCAGCGAACACATCGCTATGG
TACAGTATATGCTTATGCAGTACCGGGAGTGCCAACCGGATCCTTTTGAATACATCGCCAAGATTGTGGC
CCAGCCCACGGTGACGGCCGAATACGTTCGCAGTGTTTTGGCTGGGGTCATGTTTTGTCACACTACCCCA
GATATGTCCGGCCAACATCTTGGCTACATGGTAGCTACCTTGATCACCGCCCACGTGGTTGGGGCTGTCC
ATGACAAGGACAATCTGCGGTGGAAAAGGGTGGACACGAGTGGAGCCCTCATTCTGTTTCTGTTCCGAAA
TCTGTTCAAACAGTGGGTGTGTACGGTGAAGCGGGTCATGGAAAAATCATGCGTCGTTGGCGTCCCTGAC
CTCCAGGGGAAGTCCGGAAGTGTAAACGCCATCACAAATGTGATTACTAATGTACTCCATCTTGCTTTCG
CAACCGGTAACTGGCTCGTACGAAAGAATAGTGGCACTTCCAAGAATACTTACACACCTAGAGGGGTTAG
CCAACTGATGTGTGTTCAGAACTACGGCGCCCGAATCTCCAACCTGCGACGCATGACACATGCAGTCGGG
TTCAAGGGAAAGAATATCAAAGTAAGACAACTCCATAATTCACACAATGGTTATATCTGTCCTTACGAAA
CTCCAGAGGGCGACCGGGTCGGCAATGTAGTGCAGCTGGCTTTGTCCGCGACCATCACAGTCCCAGTCTG
TCCCAGAAACATACGGACCCTCATGATGAGCGCATTGCCTCGCTTCGCTGATCCTCAGGGTAACCAGCCG
GTACTGCTGAACGGCGAGATTGTAGGATGGGTGAAAGACAGGATGCTGTGCGGGATGGCCATCATGCATA
TGCTGTGGGATTATCCTGATGTAACCGTCGTTTTTAGGCACGGCTATGGTGTGCACGTGTATAGCGAAGG
CGGTCGCTTCATGCGCCCGCTCATCCATAGAGGGCAGTATCGAATGGTCTGTCCCTCTTTGGTGCAGGAG
CTGCTGGTACGCCCATTCCCCTCCCCCACAGAACGGGTGGTTTCAATGGATTCTATGAGGGCCGATTTCA
TGGACTTGCCCGGCATTGTTGTGTTGACCGATGCGATGAGCGCGGTAATACCATTCTACAACCACACACA
ATCACCACGAAACGCTTATCAGTCCAATATGGGCAAGCAAGCGGTGGGCCTGCCTTGTCTGAATTATATG
AGCCGCTGGGATGTCACACTGGACGTCCTTATGTACCCCCAGGTGCCACTGTCCCGCTCAAAGGCCATGG
ATGCCCTGTCATTTGATACTATGCCTCACGGTGCATGTCCCATTGTTGCCGTTCTTACCTTTGATGGATA
TAATCAAGAAGACTCTATCATTTTGAATCGCAGCTCAGTGCAGAGGGGGATGTTCGTTAGCACCACTTAC
AAAACTCTCTGTGAGTGTGAGAAACACGTGAAACGATGCGAATTCGAGAGGATCATGCGGGTTCCAGTCG
ATAAGCGAAGGCCCGGTCTGGTCTACGATTACTTGGGCGACGACGGGATTTTTGACCCTGCTCTTGCCAG
GGGAATGTTCGTGGCAGCCGGGGTGGTGGTGGTCGGGAAAATGAGCTTTGATCAATCCAGACCTGAGGGT
GCGTGTTGTAGTCTCGCCGTGAAACCACACGAGGAGGGGTACTTTGATGACAAGTTGGTCTTCAACATGG
CAGATGGATGCAAATGTGTGAAGATTAAGCTGCGCAAAATCCGCATTCCAGAAATGGGAGACAAATTTGC
AAGCTTCACGGCGCAGAAAGGAACATGCGGTATGATGTACGACCAGTGGGACATGCCCTTTACCGCTGAC
GGCGTCGTGCCCGACCTGGTGATTAATCCCCATGCCTTTCCAAGCAGGATGACTGTCCACTATCTGCTGC
AGATGTGTTTTGAACTTGGGGCCGTCGCTGGCGGGGGTAAAGGATATGACGCTACTGCATTTAACAGCGA
CGGCGCCGATCTGCTGAAGACTCTCGAAGAACTCGGATACGCTAAACAGGTCATGTATTGTGGAAGAACC
GGTCGGCGGTTTGAGTCCCTTGTGTTCATGGCGCCTTGTCCATACCAGAAGTTGAAGCATCTCGTGGCCA
ATAAGGTTCATGCAAGGACGACTGGACCCGTTGACCCTCTGACCCGCCAGCCTGTAGCCGGACGATCACG
GGATGGCGGTATTAAAATAGGGGAGATGGAGCAATGGTGCAAGATATCTCATGGGGCCGCAGCTGCACTG
CACGAAAGCATGACAACTATGTCCGACCCGTACACCGTCCCAGTGTGCTCCGTGTGCCACGTGATGACTG
ACAGTTACGACGTTTGCAGACACTGCAGGTCAGTGAGTGTGCAGACATACGCCATGCCGTACACATCTAA
ACTTTTTTTTCAGCAACTCGCCAGCATCGGGATCAAGACCAGAATCATGGAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC102324 representing NP_612256
Red=Cloning sites Green=Tags

MQAHLSYWTAPQRLVHHHIAHYEHLLRVQIPQVVEGEPPVIIHNHSVTFGDVAYSKPSPETPGHARAYDE
TYETTVSCKAYLTCMATGAKHVQHIHNLAKMPVMVRAPPLCQGIGECEKDPGGYFIVKGKERIIIPHIHT
AYNIPITMHCTTKKTLVCEMRTHNETTGNNLLVKVRLMQGCIIEMSVPYIKSFLPAGAVFNALGVSEEDM
LRYCNLAWQRTTQGGYVSEHIAMVQYMLMQYRECQPDPFEYIAKIVAQPTVTAEYVRSVLAGVMFCHTTP
DMSGQHLGYMVATLITAHVVGAVHDKDNLRWKRVDTSGALILFLFRNLFKQWVCTVKRVMEKSCVVGVPD
LQGKSGSVNAITNVITNVLHLAFATGNWLVRKNSGTSKNTYTPRGVSQLMCVQNYGARISNLRRMTHAVG
FKGKNIKVRQLHNSHNGYICPYETPEGDRVGNVVQLALSATITVPVCPRNIRTLMMSALPRFADPQGNQP
VLLNGEIVGWVKDRMLCGMAIMHMLWDYPDVTVVFRHGYGVHVYSEGGRFMRPLIHRGQYRMVCPSLVQE
LLVRPFPSPTERVVSMDSMRADFMDLPGIVVLTDAMSAVIPFYNHTQSPRNAYQSNMGKQAVGLPCLNYM
SRWDVTLDVLMYPQVPLSRSKAMDALSFDTMPHGACPIVAVLTFDGYNQEDSIILNRSSVQRGMFVSTTY
KTLCECEKHVKRCEFERIMRVPVDKRRPGLVYDYLGDDGIFDPALARGMFVAAGVVVVGKMSFDQSRPEG
ACCSLAVKPHEEGYFDDKLVFNMADGCKCVKIKLRKIRIPEMGDKFASFTAQKGTCGMMYDQWDMPFTAD
GVVPDLVINPHAFPSRMTVHYLLQMCFELGAVAGGGKGYDATAFNSDGADLLKTLEELGYAKQVMYCGRT
GRRFESLVFMAPCPYQKLKHLVANKVHARTTGPVDPLTRQPVAGRSRDGGIKIGEMEQWCKISHGAAAAL
HESMTTMSDPYTVPVCSVCHVMTDSYDVCRHCRSVSVQTYAMPYTSKLFFQQLASIGIKTRIMD

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_003494
ORF Size 3132 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_003494.1, NP_612256
RefSeq ORF 3132 bp
Locus ID 935305
MW 116.7 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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