VP1 (NC_000883) Virus Tagged ORF Clone

Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC102081 represents NCBI reference of YP_004928146 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAAAAGAAAGCGGCAAATGGTGGGAGTCTGATGATGAGTTTGCTAAGGCTGTGTATCAGCAGTTTG TGGAGTTCTATGAGAAAGTTACTGGGACTGATCTCGAGTTGATTCAGATCCTGAAGGACCACTACAACAT ATCCCTGGATAATCCACTGGAAAATCCCTCCTCACTGTTTGACTTGGTCGCTCGCATTAAGAATAACTTG AAGAATAGTCCAGATCTGTACTCCCATCATTTCCAGAGCCACGGACAGCTGTCCGATCACCCTCACGCGC TGTCAAGTTCCTCAAGTCACGCAGAACCTCGCGGAGAGGACGCAGTGCTCTCTTCTGAGGACCTCCATAA ACCCGGGCAAGTCTCAGTTCAACTCCCCGGGACCAACTACGTGGGACCTGGCAACGAACTCCAAGCGGGA CCTCCCCAGAGCGCCGTCGACAGCGCCGCAAGAATTCATGACTTCCGCTACAGTCAGCTTGCTAAACTGG GGATCAACCCCTACACCCACTGGACTGTGGCCGACGAGGAACTCCTGAAAAACATTAAAAATGAGACCGG ATTTCAAGCCCAAGTCGTGAAGGACTACTTCACACTGAAAGGGGCTGCTGCTCCAGTGGCCCATTTCCAA GGAAGCCTGCCAGAGGTTCCAGCATATAATGCTTCTGAGAAATACCCCTCTATGACATCAGTGAATTCTG CTGAAGCGAGTACAGGCGCAGGAGGAGGTGGTAGTAACCCTGTCAAAAGTATGTGGTCTGAGGGAGCCAC TTTCAGTGCAAACTCAGTTACCTGTACGTTTTCAAGGCAGTTTCTGATCCCATATGATCCTGAACACCAT TACAAGGTATTTTCACCAGCCGCATCTTCATGTCACAATGCATCAGGCAAGGAAGCTAAGGTGTGCACCA TCTCACCCATCATGGGATACTCTACACCCTGGCGGTACCTGGATTTTAATGCACTGAACCTGTTCTTCTC CCCCCTGGAATTTCAGCATTTGATCGAGAACTACGGTTCAATAGCCCCCGATGCCCTGACCGTGACTATT AGTGAGATCGCCGTCAAGGATGTGACCGACAAGACTGGTGGTGGAGTGCAAGTGACCGACTCTACCACAG GCCGGCTGTGTATGCTGGTGGACCACGAATACAAATATCCCTATGTCCTGGGCCAAGGCCAGGATACGCT TGCTCCCGAATTGCCGATTTGGGTTTACTTCCCGCCTCAGTACGCTTACTTGACCGTCGGTGACGTGAAT ACCCAGGGCATTTCCGGAGACTCCAAGAAGCTGGCTTCTGAGGAGTCAGCTTTTTATGTGCTGGAGCACT CCTCTTTCCAGCTTTTGGGAACCGGTGGGACAGCTACCATGTCCTACAAATTTCCGCCCGTACCACCAGA AAATCTGGAAGGATGTAGTCAGCATTTCTATGAGATGTATAACCCCCTTTACGGCTCTCGCTTGGGGGTG CCGGACACACTGGGCGGCGACCCTAAATTCAGATCACTGACTCACGAGGATCATGCGATCCAACCTCAGA ACTTCATGCCCGGCCCCCTCGTGAACAGCGTGTCTACCAAGGAAGGCGACTCCTCTAACACCGGAGCAGG CAAGGCACTGACCGGGCTCAGCACAGGAACAAGCCAGAACACCAGGATCAGCCTCCGGCCCGGCCCAGTG AGCCAGCCATATCACCACTGGGATACCGATAAGTACGTCACCGGTATCAACGCTATCTCTCACGGCCAGA CTACATACGGAAATGCCGAGGACAAGGAATATCAGCAGGGCGTCGGACGCTTTCCTAACGAGAAGGAGCA GCTGAAGCAGCTTCAAGGCCTGAATATGCACACATACTTTCCCAATAAAGGCACCCAACAGTACACGGAC CAGATCGAGCGCCCACTGATGGTGGGTTCTGTATGGAACAGGAGGGCGCTGCACTACGAAAGCCAGCTGT GGAGCAAAATACCTAACCTCGACGACAGCTTCAAAACTCAGTTCGCTGCCTTGGGTGGCTGGGGCCTGCA TCAGCCACCACCCCAGATTTTCTTGAAGATCCTCCCACAATCAGGACCTATTGGCGGAATCAAAAGTATG GGCATCACCACTCTGGTACAGTATGCCGTGGGGATTATGACCGTGACTATGACTTTCAAGCTCGGCCCAC GAAAAGCCACCGGGCGATGGAACCCCCAACCTGGCGTGTACCCACCACACGCAGCCGGGCACCTTCCATA CGTGCTGTATGATCCCACCGCAACGGACGCTAAACAACACCACCGCCATGGGTACGAGAAACCTGAGGAG CTCTGGACGGCCAAATCAAGGGTGCACCCACTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC102081 representing YP_004928146 Red=Cloning sites Green=Tags MSKESGKWWESDDEFAKAVYQQFVEFYEKVTGTDLELIQILKDHYNISLDNPLENPSSLFDLVARIKNNL KNSPDLYSHHFQSHGQLSDHPHALSSSSSHAEPRGEDAVLSSEDLHKPGQVSVQLPGTNYVGPGNELQAG PPQSAVDSAARIHDFRYSQLAKLGINPYTHWTVADEELLKNIKNETGFQAQVVKDYFTLKGAAAPVAHFQ GSLPEVPAYNASEKYPSMTSVNSAEASTGAGGGGSNPVKSMWSEGATFSANSVTCTFSRQFLIPYDPEHH YKVFSPAASSCHNASGKEAKVCTISPIMGYSTPWRYLDFNALNLFFSPLEFQHLIENYGSIAPDALTVTI SEIAVKDVTDKTGGGVQVTDSTTGRLCMLVDHEYKYPYVLGQGQDTLAPELPIWVYFPPQYAYLTVGDVN TQGISGDSKKLASEESAFYVLEHSSFQLLGTGGTATMSYKFPPVPPENLEGCSQHFYEMYNPLYGSRLGV PDTLGGDPKFRSLTHEDHAIQPQNFMPGPLVNSVSTKEGDSSNTGAGKALTGLSTGTSQNTRISLRPGPV SQPYHHWDTDKYVTGINAISHGQTTYGNAEDKEYQQGVGRFPNEKEQLKQLQGLNMHTYFPNKGTQQYTD QIERPLMVGSVWNRRALHYESQLWSKIPNLDDSFKTQFAALGGWGLHQPPPQIFLKILPQSGPIGGIKSM GITTLVQYAVGIMTVTMTFKLGPRKATGRWNPQPGVYPPHAAGHLPYVLYDPTATDAKQHHRHGYEKPEE LWTAKSRVHPL TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	NC_000883
ORF Size	2343 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NC_000883.2, YP_004928146
RefSeq ORF	2343 bp
Locus ID	1489598
MW	86.0 kDa

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