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E2 (NC_001690) Virus Tagged ORF Clone
CAT＃: VC101946
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for regulatory protein E2 [Human papillomavirus type 48], codon optimized for human cell expression, NP_043419
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CNY 4,750.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC101946 represents NCBI reference of NP_043419 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCCCGAGACCCAAGAATCACTGTCCACACGCTTCGCTGCTCAGCAGGAGATCCAGCTCACCCTGA TAGAGAAGGAAAGCTATGACCTCAAGGATCATCTGGCATACTGGAAAGCCGTGCGGCTCGAAAACGTGAT CGCCTACTACGCGCGCAAAGAGCACATTACTAAGCTTGGGCTGCAGCCACTTCCAACGCTGGCTGTGACA GAGTATAAAGCTAAGGAGGCGATAAATATCCAGCTGCTTATACAAAGCTTGCTGAAAAGTGAGTTTGCTT TGGAGAGATGGACCTTGGCTGAAACCTCCGCTGAAACAATTAATAGCTCACCACGAAACTGTTTTAAAAA AGTGCCCTTCATTGTCAATGTTTGGTTTGATAATGACGAAAGAAACAGCTTCCCCTACACGTGCTGGGAC TTCATTTACTACCAAGACGACCAGAACAAATGGCATAAGACTGAGGGACTGGTGGACCATAACGGTTGCT ACTACGTCGATCTCAATGGAGACTTTGTGTACTTTACATTGTTCCAACCAGACGCGGTGAAGTACGGAAA GACCGGTTTGTGGACCGTGCGGTTCAAAAACAAAACTATATCCGCTAGTGTTACCTCATCCAGCAGAAAT ACAAATCCCTCTAGCGAGAGCCGCGTCGGCCTGAGCACTTCTAGCAGCTCAGAAAGTCCCCGCAGACGGC CAAGTATCTCCGAGAACTCCAACACCGAATCACCAACCTCCTCAACAAGCAGGCTCAGGGAAAGGCGCAG AAGAGAGCCCAGAGAATCTGGCACTACTGATACTACTCCCCGGAGGAGAGGTACGAAACGCAAGTTGGGT TCCGATAGCGCCCCAACACCCTCTGAGGTGGGCAGTCGAAGTACCACCCTCGCGAGGCACGGCTATTCTC GACTTGGACGACTTCAAGAAGAAGCTCGGGATCCTCCTCTGGTGTTGTTCACCGGACAGCAAAATAACCT GAAGTGTTGGAGAAATCGGTGCACGACAAAATATGCCTCTCTTTTTTTGTGCTTCAGCAGTGTGTGGAAA TGGCTCGGACCAAATTCCGACGGGGGTGCAGCAAAAGTGCTTGTTGCCTTCAAGTCCGATGCTCAACGGC AGGTGTTTCTGAATACCGTGCATATCCCTAAGGGCACAACAATCACTCTTGGCCGGCTGGACAGTCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC101946 representing NP_043419
Red=Cloning sites Green=Tags MQPETQESLSTRFAAQQEIQLTLIEKESYDLKDHLAYWKAVRLENVIAYYARKEHITKLGLQPLPTLAVT EYKAKEAINIQLLIQSLLKSEFALERWTLAETSAETINSSPRNCFKKVPFIVNVWFDNDERNSFPYTCWD FIYYQDDQNKWHKTEGLVDHNGCYYVDLNGDFVYFTLFQPDAVKYGKTGLWTVRFKNKTISASVTSSSRN TNPSSESRVGLSTSSSSESPRRRPSISENSNTESPTSSTSRLRERRRREPRESGTTDTTPRRRGTKRKLG SDSAPTPSEVGSRSTTLARHGYSRLGRLQEEARDPPLVLFTGQQNNLKCWRNRCTTKYASLFLCFSSVWK WLGPNSDGGAAKVLVAFKSDAQRQVFLNTVHIPKGTTITLGRLDSL TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_001690 |
| ORF Size | 1188 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NC_001690.1, NP_043419 |
| RefSeq ORF | 1188 bp |
| Locus ID | 1403624 |
| MW | 45.1 kDa |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC101943 | Myc-DDK-tagged ORF clone of viral ORF for transforming protein E6 [Human papillomavirus type 48], codon optimized for human cell expression, NP_043416 |
CNY 3,800.00 |
|
| VC101944 | Myc-DDK-tagged ORF clone of viral ORF for transforming protein E7 [Human papillomavirus type 48], codon optimized for human cell expression, NP_043417 |
CNY 3,800.00 |
|
| VC101945 | Myc-DDK-tagged ORF clone of viral ORF for replication protein E1 [Human papillomavirus type 48], codon optimized for human cell expression, NP_043418 |
CNY 7,130.00 |
|
| VC101947 | Myc-DDK-tagged ORF clone of viral ORF for putative, partial [Human papillomavirus type 48], codon optimized for human cell expression, NP_043420 |
CNY 3,800.00 |
|
| VC101948 | Myc-DDK-tagged ORF clone of viral ORF for minor capsid protein L2 [Human papillomavirus type 48], codon optimized for human cell expression, NP_043421 |
CNY 6,080.00 |
|
| VC101949 | Myc-DDK-tagged ORF clone of viral ORF for major capsid protein L1 [Human papillomavirus type 48], codon optimized for human cell expression, NP_043422 |
CNY 6,270.00 |
