FRMPD3 (NM_032428) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_032428, the custom clone sequence may differ by one or more nucleotides ATGGAGACTCTGGATAGCCAGAGGGTCCAGGACCGCCTGCTGGCAGCCCCAGGATGCAGCTCCCCTTCTG GCCAACAGGAACTCTTTTCCAGTCATGTGATGCAGGAAGAGAGCGCAAATGATATGGAATGTGAGCAGCT GCCAGCAGAGATACTGCGACAAGTGACCGTTCACCGAGACCCTATATATGGCTTTGGCTTCGTGGCTGGC AGTGAGAGGCCTGTGGTGGTTCGATCTGTGAGGCCAGGAGGCCCCTCTGAGAACAAGCTCCTGGCTGGTG ACCAGATTGTGGCTATTAATGAGGAAGACGTGAGTGAAGCCCCGAGGGAGAGACTCATAGAACTTATCAG GAGCGCTAAGGAATTCATCGTTCTTACAGTTCTGCACACTCATCAGTCCCCCAAATCTGCTTTCATCAGT GCTGCGAAGAAGGCCAAGTTGAGGTCCAATCCTGTGAAGGTTCGATTTTCTGAGCAGGTGGCAGTTGGAG AAACAGATGCAAAAATGATGAAGAAGGAAGCTCTCCTCCTCATCCCTAATGTCCTGAAGGTTTTCTTAGA AAATGGGCAGATCAAGTCATTCACATTTGATGGTCGGACCACTGTTAAGGATGTGATGTTGACATTACAG GACCGCCTTTCCCTGAGGTTCATTGAGCACTTTGCTCTTGTCCTTGAGTATGCCGGGCCAGAACAGAATC ACAAGTTTCTGCTTCTTCAGGACAAGCAACCCCTGGCTTATGTGGTACAGCGGACACACTATCATGGAAT GAAATGCCTCTTCCGAATAAGCTTCTTTCCCAAAGACCCTGTGGAGCTGCTGCGTCGGGATCCTGCTGCT TTTGAGTACCTATACATCCAGAGTCGGAATGATGTTATTCGAGAACGCTTTGGAATGGATCCCAAGCCAG AGATGCTTTTGGGCCTTGCTGCGCTCCACATCTATATCACTGTCTCAGCCACTCGACCTAGTCAGAAGAT CTCGCTCAAGAATGTGGAGAAGGAGTGGGGCCTGGAACCCTTTCTTCCCCCCTCCCTCCTGCAGGTCATC AAAGAGAAGAACCTCCGGAAATCTCTCTCTCAGCAACTGAAGGCTCACCAAACACATCCTTCCTGCGGCA CCAAGGGCTCTGCAATTCAGGCAAAGCTCCAGTATCTACGAATTCTGAATGAACTTCCTACCTTCACGGG CGTTTTGTTCAACACTGTAGGCCTGGATGAGAAGCAGTCGGCCACCACGCTCCTGGTGGGACCCCGTCAT GGCATCAGCCATGTTATTGACCTCAAAACCAACCTCACCACTGTGCTGTCCGAGTTCAGCAAGATCAGCA AGATCCAGCTGTTCCGGGAGAACCAGGGCGTGGCCCGGGTGGAGACCAGCATCATGGATGCCAAGCCTCT GGTGCTGTTGATGGAGTGGCCTGAAGCCACCAACTTTGCCTGCCTGATCGCGGGGTACTGCCGCCTCTTG CTGGATTCCAGGAAGATGGTCTTCTCCAGGCCTGCCAGCCAGCCACTTCCACCTCCAATGATCAAGGCAG ATTACATGCACAGCGCCCACCGCCCTGTCACTGGGGGCCACCTGGGGAAAAAGGAGAGTAGTTATGTGGG CAGCGTGGGCACCAGCCCCAGGAAATCGAGCCGCTGCACGCCCCCACCTGCCGACTCTGAGCTTGTCAGC TTCTGCTACCTCCATATGCGGGAACAAAGGAAGGAGCAGGAAAGCCGGACAGATGTCAACGAGAACCTAA TCTTCTTTGAGGAGACCAGGCCCCGAACCAAGTCTGACCCCACATCCAAAAGCTCTGGCCAAGGTTATGA GGTAGTCCCTGATGACTTTGATGCAGCTAGCCTAGACCACGAGCCTTGTGCCAGCAGGGCCCGGTCCTAC ACCTTGGACAATTCCCTTGGGGCTGAAGCCCTGAATTTCTACTGTGACTCTTGCAAAGCCAAACTTCAGG AGCAGCTGGGCCCTCGCAAAGGTGGGAAGCCTGGCTCCTCTCGTGACAATATAGTAGATTTGATGTCCCT CCCACCACCTGGGAGTGAGGAGGAGGAGGAGGAGGAAGATGAGACAACTTCTCTGTTGCCAGCCATTGCT GCCCCACCCCCTGGTTTCCGAGACAACAGCTCTGATGAGGATGACCCCAAGCGCCGGGCTGTCCAGAGCC AGGAACAAGGACGCCACCTGCGTGGGCTTCTGTACGATGAGATTCCAGTGACATTGATTGACAGTGTGCA GACCCGGACAGTTCGAGATCATGCCCAGGAGCTAGATGATGCCCTGGTGTCCACTCTGCAGGCTCTAGAA GCCCTGGCTGCATCCGAGGATGGACCACACCCACCACCCCCACAGACTGCAGGTCTGATTGTGCTGGCCA CAATCACTCCTGAATCATCGCTGGACTCAGGTCATGAAACCAACTCTTCAGAGCTCACAGACATGTCAGA GATGATGTCGGCCATGAAGCAGCACCAGAACACCACCTACTTCCTGGCCCAGCACCTCAACAAGGACAGC CTCCTTGCCCGCAAGGACCTGCCCTTCCGGATCCAGAGCTGTGCAGCCCAGGCCGTTCTTACGGCCCCTT ACTCTCTTGGGCGCCCGGATCCCAACCCATCTCTCCAACCCATTGCCACAGGCCAGAGTCCTGGCCCCCC TGGCGCTCGGAGGAAGCTGCCCCAGTCAGAGGGCCAGGTACAGGGAGAACGAACATACTCCTTGGCAGTG CACCCAGCACTGTCCCCACAGCTTAGTGAACAGAAGAATCTGAGTCTGCTGTCCCCAGTTCCTGAGGACA AAGGGCCTGGCCACACTAGGGCAGGCCTAGAAATGTCACTGAGGGCAGCCACATCATCCCTCAGTGAAGA GCAGGTCTCTGAGCTGAGGGACAACCTGCCCAAGGAGGTCAGGTTGAGCCCCAAGCTTATCCTCGACCCA AAGAGCAGTGTGACCCCTGCCATCATCTCGGCCGCCCTACAGCAAGTGGTTCACAATAAGAGTCTAGTCA CTGCTGGTGGGGCTTTGGGGAACCCCCCCAGCAGGGGTGAGAGAAGGCTGGAGGCCAGCATGGGGAGGCC AGAGGTTAGCATGATGAGCAGCAGTGCCAGTAAGAATCTGAAGTTCAAAATTAGCCCCAGTGCTCCAGAG ACCTCATGGAATTCTCAACATCAGCTGGGTGCAGAGGTCTCTTCCAGCCCCAGAGCACCCACAGGCAGCC GGGCTGACAGCCTGCACCTCTCCCAACAAGAGGACAGTCTGCCTGTTCAAAATTTCCCTCCCAAAAGCTA TCTTTTGCGAACAAGCCGAGAGTCAGTGGGCAAGCAAGCTACAGGGGAGGTGGCAGGCAAAGGCGGGCCA GTGGGTGGTAAGCCCACCCTGCAGAAGCAGGGCACCATCTCCAGCCAAGGGGAGAAGGCGCAGCTGGAGA GCACACCCAAAAGAAGCAAGCTCGAAGAGACCAGCCTGGTTCCCCGAGCTACCTACCCCATGGCTCTGCA GAGCCCCAGCTGCCAGTCAAGAAGCCACAGCCCCAGCTGCCAGCCTCATGGCCACAGCCCCAGCAGCCAG TCTCGAGGTCAGAGCCCCAGCTGCCAACCTCGAGGCCAGAGCCCACTGAGGTCTCAGGCTGCCAGCCGGC AGGTGAGCACCATGCCCTCTAGGAAGCTTGAAACAACTCTCAATGGAGCCCACTCGACCTCTGAAGGCCC TGCCAAACCCAAGTCATCCCGAGGTCCTTTCCGGCTACGCAATTTATTCTCTGCCACCTTCCCAACCCGC CAGAAGAAGGAGACAGATGAGCGGCAGGCCCAACTGCAGAAGGTAAAGCAGTATGAACTGGAGTTCCTTG AGGAACTTCTAAAGCCACCAAGCCAGGGGGAGCTGCCAGGCACCGAGTACCTGCAACCTCCAGCACCTGG CCGCTGCAGCTGCCAGCTCCGCAGCAGCCCTGTGCAGCAGGGGCCTGGCATGTCCCGTGAGCAGAGGCGC AGCTGTGACTGCAAGCGCATCTGCCGGGGGGGCCGGCCACAAGCCACCCAGACACCAGTGCCCAGCCTCC GGGGGAGGGAAAGGGACAGAGTCCTCCCTAGCCAGAGGCAGCCAGAGGCTGGCCCAGGCGTGAGCCTCAG CAGCCCCATCAATGTCCAGCGCATTCGTTCTACCAGCCTGGAGTCCCGAGAGTGCCGATCGGACCCTGAG AGTGGTGTTTCGTGCCTGACCACGTGTGCCTCGGGGGGCGAGTGTCTGGGAGCTCCCAATTACAGGAAAC TGATGCGCCGCTACAGTATCAGTGAGCTGGACCAGGGTGACAGGGCCTCGCTGACCTCGGATGTCTACCC ACATCCTCCCCTGGGCATGCTGCCCAGGGAGGCCAAGGAGGTAGAGGCAAGCCTCCCCATAGCCTTGGGT CCCAAAAGCAGGTCTCTGGAGTCACCGACGCTGGGAGACCCCTCCTACGTCCAGGTTGCCCCAGAGACCA AAGGCCCCAGACAGATGGCCGTGTTCTCACTGCCCGAGGAGGTGTACCGGAAGCCTGCCGAGCTAGACGA GGACAGTGAGAGCAGCAAGTGCTGCTCCATCCGCTACTGCTTCTACTACCGCAAGTGTGACATGGCAGAT GATGCCAGTGATGGCAAGGATGAGCTCTCCTACTCTATCCCCATGAAGATCCTGCCTGGCATGAAGCTGG ACGAGCAGGTGGTGCCTGTGGTGAGCAGGACCCTGCAGGTGCTGGATGCTGCTACCTGCAGCAGCAGCAG CCCTGAGGCCTCCCGCACTCAGGAGATTGACCTCCGTGTGTCCACCTTCGAGGGGAGCCTGGCCAAGATC AATGCCCTGCGGGCCCATGCCTATGGCCTCCCTGATGGCTTCCTGGCTGCCCGGCTGGACACCAACGAGC TGCTGACAGTCCTGCGGCAGTGTGTGGCCAGCCCCGAGGCCCGTGCCCCCAAGCCCTATGTGTCTCAGAT CTCCGAGTATAAGCTTGAGCTAGCTCTCAAGTTCAAGGAGCTCCGGGCCTCCTGCCGCCGTGTGGCCAAT GTGGACAAGAGCCCAACTCACATGCTGGCAGCCATCACGGGCAGCTTCCAGGTGCTGAGCAGCCTCATTG AGACCTTCGTGCGGCTGGTGTTCATTGTGCGCTCCGAGGCCCAGCGCCAAGAGCTGCTGGCCAAGGTAGA AGAGGTGGTGAGGAACTACACCTTCCTGCTGCGTGCAGCTGAGGAGTCCACAGCCCGTAACCTTAACCAG CAGCAGCAGCAACAACAACAGCAGCAGCAGCAACAACAACAGCAGCAGCAACAACAACAGCAGCAGCAGC AGCAGCAGGTGGCAGCAGCTGCAGGGGCAGCCACAGAGCATCCACCAGGCTCCCCAACTTCGGCGACTGT TATGAGCACATTCACCCACTCCTTAAAAACCCTTATTAAGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_032428
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_032428.1, NP_115804.1
RefSeq Size	7304 bp
RefSeq ORF	5433 bp
Locus ID	84443
UniProt ID	Q5JV73
Gene Summary	This gene encodes a protein that contains a PDZ (post synaptic density protein (PSD95), Drosophila disc large tumor suppressor (Dlg1), and zonula occludens-1 protein (zo-1) domain at the N-terminus followed by a FERM domain. The encoded protein is involved in signal transduction. The PDZ domain is thought to function in protein-protein interactions, mainly by binding to specific C-terminal peptides of other proteins. The FERM domain is found in proteins that are localized to the plasma membrane and are associated with the cytoskeleton. [provided by RefSeq, May 2017]

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