PHRF1 (NM_001286583) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PPP1R125; RNF221
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001286583, the custom clone sequence may differ by one or more nucleotides ATGGATGACGACAGCCTGGATGAGCTTGTGGCCCGGAGCCCAGGGCCGGATGGACACCCACAGGTCGGCC CTGCGGACCCGGCAGAAAGCAGCGTGGGCAGCAGTGGGGACTCTGGGGACGACAGTGACAGCGAGCATGG AGATGGCACAGACGGAGAAGACGAGGGGGCGTCTGAGGAGGAAGACCTGGAAGACAGATCTGGTTCCGAG GATTCTGAAGACGACGGGGAGACATTGCTGGAGGTAGCGGGTACTCAGGGGAAACTGGAAGCCGCTGGCT CTTTCAATTCTGATGATGATGCAGAGAGCTGCCCAATCTGTCTCAACGCATTCAGAGACCAGGCCGTGGG GACGCCGGAGAACTGTGCCCATTACTTCTGCCTGGACTGCATTGTCGAATGGTCCAAGAATGCCAATTCC TGTCCAGTTGATCGAACTCTATTTAAGTGCATTTGTATTCGAGCTCAATTTGGTGGTAAAATCTTAAGAA AGATCCCAGTGGAGAACACCAAAGCGAGCGAGGAGGAGGAGGACCCGACCTTCTGTGAGGTGTGCGGCAG GAGCGACCGTGAGGACAGGCTTTTGCTCTGCGACGGCTGCGATGCGGGGTACCACATGGAATGCTTGGAC CCCCCTCTCCAGGAGGTGCCGGTGGACGAGTGGTTCTGCCCGGAATGTGCTGCGCCTGGTGTTGTCCTTG CCGCTGATGCGGGTCCCGTGAGTGAGGAGGAGGTCTCCCTGCTCTTGGCTGATGTGGTGCCCACCACCAG CAGGCTTCGGCCTCGAGCAGGTAGGACCCGGGCGATAGCCAGGACACGGCAGAGTGAGAGAGTGAGAGCA ACCGTGAACCGGAACCGGATCTCCACGGCCAGGAGGGTCCAGCACACACCAGGGCGCCTCGGGTCTTCCC TGCTGGATGAAGCCATCGAGGCTGTGGCGACTGGCCTGAGCACTGCCGTGTATCAGCGCCCCCTGACGCC GCGCACTCCCGCCCGACGGAAGAGGAAGACAAGAAGACGGAAGAAAGTGCCGGGAAGAAAGAAAACCCCG TCCGGACCATCCGCAAAAAGTAAGAGCTCAGCGACAAGATCTAAGAAACGCCAACATCGAGTGAAGAAGA GAAGAGGGAAGAAGGTAAAGAGTGAAGCCACCACTCGCTCTCGAATCGCGCGGACGCTGGGCCTGCGCAG GCCTGTTCACAGCAGCTGCATCCCGTCAGTGTTGAAGCCAGTGGAGCCCTCTTTGGGGCTGCTGAGAGCG GATATTGGAGCTGCCTCTCTGTCTCTGTTTGGAGATCCTTATGAGCTGGATCCCTTCGACAGCAGTGAAG AGCTTTCTGCAAACCCTCTTTCCCCTCTGAGTGCCAAGAGACGGGCTCTGTCCCGGTCAGCCCTGCAGTC CCACCAGCCCGTGGCCAGGCCCGTCTCCGTGGGGCTTTCCAGGAGGCGCCTCCCTGCCGCGGTGCCAGAG CCAGACTTGGAGGAGGAGCCAGTGCCTGACCTGCTGGGCAGCATCCTGTCGGGCCAGAGCCTCCTGATGC TGGGCAGCAGTGATGTCATCATCCACCGCGACGGCTCCCTCAGCGCCAAGAGGGCGGCTCCAGTTTCTTT TCAGCGAAACTCAGGCAGTCTGTCCAGAGGGGAAGAAGGATTCAAGGGCTGCCTGCAGCCCCGAGCACTG CCCTCCGGGAGCCCGGCCCAAGGCCCGTCAGGAAACAGGCCACAGAGCACAGGGCTCAGCTGTCAAGGCA GGTCCCGCACCCCCGCCCGCACCGCGGGGGCGCCTGTGAGGCTGGACTTGCCAGCAGCCCCTGGGGCGGT TCAGGCTCGGAACTTGTCAAATGGGAGTGTGCCTGGCTTCAGACAGAGCCACAGCCCCTGGTTCAACGGC ACCAACAAGCACACCTTGCCCCTTGCCTCTGCCGCGTCTAAGATCTCAAGCAGAGATTCTAAGCCCCCAT GTCGCAGTGTGGTGCCGGGGCCTCCCCTGAAGCCAGCGCCCAGAAGAACAGACATCTCTGAGCTACCCAG GATACCAAAGATCAGGAGAGATGACGGTGGTGGCAGACGGGATGCGGCCCCGGCCCACGGGCAGAGCATT GAGATCCCCAGTGCCTGCATCAGCCGACTGACTGGCAGGGAGGGCACCGGGCAGCCAGGGCGAGGCACAC GGGCAGAGAGCGAGGCCAGCAGCAGGGTGCCCCGGGAGCCCGGGGTGCACACGGGCAGCTCCCGGCCCCC AGCCCCCAGCTCCCATGGCAGTTTGGCCCCACTGGGACCATCAAGAGGGAAAGGGGTCGGGTCGACCTTT GAGAGCTTCCGGATCAATATTCCTGGAAACATGGCACATTCCAGCCAGCTCTCCAGCCCTGGCTTCTGTA ACACGTTCCGGCCTGTGGACGATAAGGAGCAGAGGAAGGAGAACCCCTCACCCCTCTTCTCCATCAAGAA GACGAAGCAGCTGCGGAGCGAGGTCTACGACCCATCCGACCCCACCGGCTCCGACTCCAGCGCCCCTGGC AGCAGCCCCGAGAGGTCTGGCCCCGGCCTCCTGCCCTCTGAGATCACACGAACCATCTCCATCAACAGCC CGAAGGCCCAGACGGTGCAGGCTGTGCGCTGCGTCACCTCCTACACGGTGGAGAGCATCTTTGGTACAGA GCCCGAACCCCCTCTCGGACCGTCCTCCGCCATGTCCAAGCTCCGGGGTGCAGTGGCTGCCGAGGGGGCC TCTGACACGGAGCGAGAGGAGCCCACAGAGAGCCAGGGCCTGGCTGCCCGGCTGCGGAGGCCATCCCCCC CAGAGCCCTGGGATGAGGAGGATGGGGCGTCTTGCAGCACCTTCTTTGGCTCTGAGGAGCGGACGGTGAC CTGTGTGACTGTCGTGGAGCCGGAAGCCCCACCCAGCCCGGACGTGCTGCAGGCTGCCACCCACAGAGTC GTGGAGCTCAGGCCCCCTTCCCGGTCCCGCTCCACATCCAGCTCCCGCAGCAGGAAGAAGGCCAAGAGGA AGAGGGTGTCCAGGGAGCACGGACGGACGCGCTCTGGGACGCGCTCTGAATCCAGGGACAGGAGCTCGAG GTCAGCGTCACCATCAGTGGGTGAGGAGCGCCCCAGGAGGCAGCGGTCCAAGGCCAAGAGCCGGCGGTCC TCCAGTGACCGCTCCAGCAGCCGAGAGCGAGCTAAGAGGAAGAAAGCCAAGGACAAGAGCAGGGAGCACA GGCGGGGCCCCTGGGGCCACAGCCGGAGGACGTCCCGGTCGCGGTCGGGGAGCCCTGGCAGCTCTTCCTA TGAGCACTATGAGAGTAGGAAGAAGAAGAAAAGGAGATCAGCGTCCAGACCTCGGGGAAGGGAGTGCTCC CCCACCAGCAGCCTGGAGAGGCTCTGCAGGCACAAGCATCAGCGGGAACGCAGCCACGAGCGGCCAGACA GGAAGGAGAGTGTGGCGTGGCCCCGAGACCGGAGGAAGCGGAGGTCCCGGTCCCCAAGCTCGGAGCACAG GGCACGGGAGCACAGGCGGCCTCGGTCCCGTGAGAAGTGGCCGCAGACCCGGTCCCATTCCCCAGAGAGG AAGGGGGCTGTGAGGGAGGCTTCCCCAGCGCCCCTTGCACAGGGGGAGCCAGGGCGGGAAGACCTCCCCA CCAGGTTGCCAGCCTTGGGGGAAGCACATGTCTCGCCGGAGGTGGCTACGGCCGACAAGGCCCCCCTGCA GGCTCCCCCTGTCCTGGAGGTGGCAGCTGAGTGTGAGCCGGACGACCTGGACCTGGATTATGGCGACTCC GTGGAGGCCGGACACGTCTTTGATGATTTCTCAAGCGACGCCGTTTTCATCCAGCTCGATGACATGAGCT CGCCACCTTCTCCCGAAAGCACAGACTCTTCCCCGGAGCGAGACTTCCCACTGAAGCCTGCGTTGCCCCC AGCCAGCCTGGCCGTGGCCGCCATCCAGAGGGAGGTGTCATTGATGCACGATGAAGACCCTTCGCAGCCC CCACCCCTGCCAGAGGGCACCCAGGAGCCACATTTGCTCAGGCCGGACGCGGCTGAGAAGGCTGAGGCAC CCAGTTCCCCGGATGTGGCGCCTGCGGGGAAGGAAGACAGCCCCTCTGCGAGTGGGAGGGTACAGGAGGC AGCCCGGCCTGAGGAGGTGGTTTCGCAGACCCCCCTGCTGCGGTCCAGAGCCCTGGTGAAGCGGGTCACC TGGAACCTGCAGGAGTCGGAGAGCAGCGCCCCCGCCGAGGACAGAGCCCCCCGGGCACCACTTCACAGGC CACAGAAGCCCCGAGAAGGAGCCTGGGACATGGAGGATGTGGCCCCCACAGGGGTCAGGCAGGTGTTCTC CGAGCTGCCCTTTCCCAGTCACGTGCTTCCGGAACCCGGGTTCCCAGACACAGACCCCTCTCAGGTTTAC AGCCCCGGCCTGCCGCCTGCCCCGGCCCAGCCCTCAAGCATCCCACCCTGCGCACTGGTCAGCCAGCCCA CGGTCCAGTTCATCCTTCAGGGGAGCCTGCCGCTAGTGGGCTGTGGGGCAGCACAGACCCTGGCCCCAGT GCCCGCTGCCCTGACCCCAGCCTCAGAGCCAGCCAGTCAAGCCACTGCAGCCAGCAACTCGGAGGAGAAG ACCCCGGCCCCCAGGCTAGCTGCGGAGAAAACCAAGAAGGAGGAGTACATGAAGAAGCTGCACATGCAGG AGCGTGCTGTGGAGGAGGTGAAGCTGGCCATCAAGCCCTTCTACCAGAAGAGGGAGGTGACCAAGGAGGA GTACAAGGACATCCTGCGCAAGGCCGTGCAGAAGATCTGCCACAGCAAGAGTGGAGAGATCAACCCCGTG AAGGTGGCCAACCTGGTGAAGGCGTACGTGGACAAGTACAGGCACATGCGCAGGCACAAGAAACCAGAGG CCGGGGAGGAGCCGCCCACGCAGGGGGCCGAGGGCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001286583
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286583.1, NP_001273512.1
RefSeq Size	5567 bp
RefSeq ORF	4938 bp
Locus ID	57661
UniProt ID	Q9P1Y6
Protein Families	Druggable Genome

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