CAMSAP1L1 (CAMSAP2) (NM_001297708) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CAMSAP1L1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001297708, the custom clone sequence may differ by one or more nucleotides ATGGGGGATGCTGCAGACCCCAGGGAGATGAGAAAGACGTTCATTGTTCCAGCCATCAAGCCTTTTGACC ACTATGATTTCTCCAGGGCCAAAATCGCCTGCAATCTGGCCTGGCTGGTGGCCAAAGCCTTTGGGACAGA AAATGTGCCAGAGGAACTTCAAGAACCATTTTACACAGATCAGTATGACCAGGAACACATCAAACCACCT GTTGTTAATTTGCTTCTATCGGCTGAACTATACTGTCGTGCTGGGAGTCTCATTCTCAAGAGTGATGCTG CAAAACCCCTTTTGGGCCATGATGCTGTAATCCAGGCTTTAGCACAGAAAGGTCTTTATGTCACTGACCA GGAAAAATTGGTAACTGAACGAGATCTCCACAAGAAACCCATACAGATGAGTGCACATTTGGCCATGATC GATACCCTCATGATGGCTTATACTGTAGAAATGGTCAGTATAGAAAAAGTAATTGCGTGTGCTCAGCAGT ATTCAGCTTTTTTTCAAGCCACAGATCTGCCCTATGATATTGAGGACGCTGTCATGTACTGGATAAATAA GGTAAATGAACATTTGAAAGACATAATGGAACAAGAACAAAAACTGAAAGAACATCACACAGTTGAAGCT CCAGGAGGTCAAAAGGCTCGTTATCGGAAAGAGCAAACATTGCTTAAGCAACTGCCTTGCATTCCATTGG TAGAAAATTTGTTGAAGGATGGGACAGATGGCTGTGCATTAGCTGCCCTTATTCATTTTTACTGTCCTGA TGTTGTCAGATTAGAGGATATTTGTTTGAAAGAAACTATGTCTTTGGCTGATAGCCTGTATAATCTGCAG CTGATTCAAGAATTTTGCCAAGAATACTTGAACCAGTGTTGCCATTTCACTCTGGAAGATATGCTCTATG CTGCTTCATCCATAAAGAGTAATTATTTGGTGTTCATGGCGGAACTGTTCTGGTGGTTTGAAGTGGTGAA GCCGTCTTTTGTACAGCCTCGTGTTGTTCGTCCACAAGGAGCTGAACCTGTAAAAGATATGCCTTCAATT CCTGTCTTGAATGCTGCCAAAAGAAATGTCTTAGATAGTAGTTCTGACTTCCCTTCAAGGTATTCACGTC CCCAGGCTCATTCTTCAGCCTCAGGAGGAATTAGAAGGTCTTCATCTATGTCTTATGTTGATGGCTTCAT AGGGACATGGCCCAAAGAGAAAAGATCATCAGTGCATGGCGTATCATTTGATATTTCTTTTGATAAAGAA GATAGTGTACAGAGATCCACTCCAAACCGAGGAATCACTCGTTCTATTAGTAATGAAGGACTTACTCTGA ACAACAGTCATGTATCTAAACACATTAGGAAAAATTTGTCCTTCAAGCCAATAAATGGAGAAGAGGAAGC AGAGAGCATTGAAGAAGAACTTAATATAGATTCTCACAGTGACCTCAAATCTTGTGTGCCCCTTAACACA AATGAACTAAATTCTAATGAGAATATTCATTACAAGCTTCCAAATGGAGCTTTACAAAATAGAATACTTC TTGACGAGTTTGGCAATCAGATCGAGACACCAAGCATTGAAGAAGCATTACAAATAATTCATGATACTGA AAAATCTCCTCATACACCTCAGCCAGACCAAATTGCTAATGGCTTCTTTCTTCATAGTCAAGAAATGAGT ATCTTAAATTCAAATATCAAGTTAAATCAATCTAGTCCTGATAATGTAACTGATACGAAAGGTGCCTTGA GTCCCATAACTGACAATACTGAAGTAGACACTGGAATTCACGTTCCTTCAGAAGATATTCCTGAAACTAT GGACGAAGATTCTTCGTTGAGAGATTATACTGTAAGCTTGGACTCTGACATGGATGATGCATCTAAATTT CTTCAGGATTATGATATTCGAACTGGCAACACCAGGGAAGCTTTGAGTCCTTGTCCAAGTACTGTAAGTA CCAAGTCTCAGCCAGGCAGCAGTGCTTCTTCTAGTTCTGGAGTTAAAATGACCAGCTTTGCTGAACAAAA ATTCAGGAAACTGAATCATACCGATGGAAAAAGTAGTGGAAGCAGTTCTCAAAAAACTACACCAGAAGGC TCTGAACTTAATATTCCTCATGTGGTTGCTTGGGCACAAATTCCAGAAGAAACAGGGCTTCCACAGGGAC GGGACACTACCCAGCTGTTGGCCTCTGAAATGGTGCATCTTAGGATGAAACTAGAAGAAAAGAGGCGTGC TATAGAAGCCCAGAAAAAGAAAATGGAAGCTGCTTTTACCAAACAGAGACAGAAAATGGGAAGGACAGCA TTCCTTACTGTAGTGAAAAAGAAAGGGGATGGGATATCTCCTCTACGAGAGGAAGCGGCGGGTGCAGAAG ATGAGAAAGTATATACTGATCGAGCAAAAGAAAAGGAATCACAAAAAACTGATGGACAAAGGAGCAAGTC ACTGGCAGATATAAAAGAGAGCATGGAGAATCCTCAAGCCAAATGGCTAAAGTCTCCAACTACACCTATT GATCCTGAGAAGCAGTGGAACCTGGCAAGCCCCTCAGAAGAAACTTTAAATGAAGGAGAGATTTTAGAAT ATACCAAATCCATTGAAAAGTTAAATTCATCCCTGCATTTTCTACAACAAGAAATGCAACGCTTGTCACT TCAGCAGGAGATGTTAATGCAGATGAGAGAGCAACAATCTTGGGTGATTTCACCTCCACAACCCTCTCCA CAGAAACAGATTCGAGATTTTAAGCCTTCTAAGCAGGCAGGCCTGTCATCAGCCATTGCACCATTCTCCT CAGACTCCCCTCGTCCTACTCACCCATCTCCACAGTCTTCTAACAGGAAAAGTGCATCTTTTTCTGTTAA AAGTCAAAGGACTCCTAGGCCAAATGAGTTAAAAATAACACCTTTGAATCGAACCTTGACACCTCCTCGG TCTGTGGATAGCCTTCCTCGGTTAAGGAGGTTTTCACCAAGTCAAGTTCCTATTCAAACTAGGTCATTTG TATGTTTTGGGGATGATGGAGAACCTCAGTTAAAGGAATCCAAACCTAAAGAGGAAGTTAAAAAGGAGGA ATTGGAATCCAAAGGGACTTTGGAACAGCGTGGACATAATCCAGAAGAAAAGGAAATCAAACCTTTTGAG TCAACAGTCTCTGAAGTCCTATCACTGCCTGTCACAGAGACTGTATGTCTGACACCAAATGAGGACCAAT TGAATCAACCCACAGAACCCCCTCCTAAACCCGTTTTCCCACCCACTGCTCCAAAAAATGTTAATCTGAT TGAAGTTTCCCTCTCAGATTTGAAACCCCCTGAAAAGGCTGATGTACCTGTTGAAAAATATGATGGAGAA AGTGATAAAGAACAATTTGATGATGACCAGAAAGTATGCTGTGGATTCTTTTTTAAGGATGATCAAAAAG CAGAAAATGATATGGCAATGAAACGGGCAGCTTTGTTGGAGAAAAGATTAAGAAGGGAAAAGGAAACTCA GCTCCGGAAACAACAGTTGGAAGCAGAAATGGAGCATAAGAAGGAGGAAACAAGGCGTAAAACTGAGGAA GAACGTCAGAAGAAAGAAGATGAGAGAGCACGCAGAGAATTTATTAGGCAAGAATATATGAGGCGGAAAC AACTGAAACTAATGGAAGATATGGATACAGTAATTAAACCCCGTCCTCAAGTAGTAAAACAAAAAAAACA GCGACCAAAATCTATTCACAGAGATCATATTGAATCCCCCAAAACACCAATAAAGGGTCCTCCAGTCTCT AGCCTTTCTTTGGCATCGCTGAACACGGGTGATAACGAGAGTGTACATTCAGGCAAGAGGACGCCAAGAT CAGAGTCTGTAGAAGGCTTCTTATCTCCAAGTCGTTGTGGCAGTCGAAATGGAGAAAAAGACTGGGAGAA TGCATCAACAACTTCTTCAGTGGCTTCTGGAACAGAATATACAGGACCAAAGCTCTACAAAGAACCCAGT GCAAAATCCAATAAGCACATAATACAAAATGCTTTAGCTCATTGCTGTTTGGCTGGAAAAGTAAATGAAG GTCAGAAGAAAAAAATACTGGAGGAAATGGAGAAATCAGATGCCAACAACTTCTTAATCTTGTTCCGGGA TTCAGGATGCCAGTTCAGATCTTTATACACTTATTGCCCAGAAACTGAAGAAATCAATAAACTGACTGGG ATAGGCCCTAAATCTATCACTAAAAAAATGATTGAAGGACTTTACAAATATAATTCTGACAGGAAACAGT TTAGCCACATACCCGCTAAAACTTTATCTGCCAGTGTTGATGCAATTACCATTCATAGCCATTTATGGCA GACCAAAAGACCAGTAACACCCAAAAAACTTTTACCCACTAAGGCATAG
Restriction Sites	SgfI-MluI
ACCN	NM_001297708
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001297708.1, NP_001284637.1
RefSeq Size	7321 bp
RefSeq ORF	4389 bp
Locus ID	23271
UniProt ID	Q08AD1
Gene Summary	Key microtubule-organizing protein that specifically binds the minus-end of non-centrosomal microtubules and regulates their dynamics and organization (PubMed:23169647, PubMed:24486153, PubMed:24706919). Specifically recognizes growing microtubule minus-ends and autonomously decorates and stabilizes microtubule lattice formed by microtubule minus-end polymerization (PubMed:24486153, PubMed:24706919). Acts on free microtubule minus-ends that are not capped by microtubule-nucleating proteins or other factors and protects microtubule minus-ends from depolymerization (PubMed:24486153, PubMed:24706919). In addition, it also reduces the velocity of microtubule polymerization (PubMed:24486153, PubMed:24706919). Through the microtubule cytoskeleton, also regulates the organization of cellular organelles including the Golgi and the early endosomes (PubMed:27666745). Essential for the tethering, but not for nucleation of non-centrosomal microtubules at the Golgi: together with Golgi-associated proteins AKAP9 and PDE4DIP, required to tether non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed:27666745). Also acts as a regulator of neuronal polarity and development: localizes to non-centrosomal microtubule minus-ends in neurons and stabilizes non-centrosomal microtubules, which is required for neuronal polarity, axon specification and dendritic branch formation (PubMed:24908486). Through the microtubule cytoskeleton, regulates the autophagosome transport (PubMed:28726242).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks an alternate in-frame exon and uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (3) is shorter than isoform 1.

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