SSH2 (NM_001282129) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	SSH-2; SSH-2L
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001282129, the custom clone sequence may differ by one or more nucleotides ATGGCTTTGGTCACGGTCCAGCGGTCACCTACCCCCAGCACCACCTCCAGCCCCTGCGCCTCGAGCTCTC ATTTGGAAGACAGTGAATCAGCAGCATTACTTTGCTGTGAATGTGAAGAATCAGAAATCTTTACTGATTC CAATGAGGCAGACAGTGGGGAGGAAGAATGCCGGTCACAGCCCAGGAGCATCAGCGAGAGCTTTCTAACT GTCAAAGGTGCTGCCCTTTTTCTACCACGGGGAAATGGCTCATCCACACCAAGAATCAGCCACAGACGGA ACAAGCATGCAGGCGATCTCCAACAGCATCTCCAAGCAATGTTCATTTTACTCCGCCCAGAAGACAACAT CAGGCTGGCTGTAAGACTGGAAAGTACTTACCAGAATCGAACACGCTATATGGTAGTGGTTTCAACTAAT GGTAGACAAGACACTGAAGAAAGCATCGTCCTAGGAATGGATTTCTCCTCTAATGACAGTAGCACTTGTA CCATGGGCTTAGTTTTGCCTCTCTGGAGCGACACGCTAATTCATTTGGATGGTGATGGTGGGTTCAGTGT ATCGACGGATAACAGAGTTCACATATTCAAACCTGTATCTGTGCAGGCAATGTGGTCTGCACTACAGAGC TTACACAAGGCTTGTGAAGTCGCCAGAGCGCATAACTACTACCCAGGCAGCCTATTTCTCACTTGGGTGA GTTATTATGAGAGCCATATCAACTCAGATCAATCCTCAGTCAATGAATGGAATGCAATGCAAGATGTACA GTCCCACCGGCCCGACTCTCCAGCTCTCTTCACCGACATACCTACTGAACGTGAACGAACAGAAAGGCTA ATTAAAACCAAATTAAGGGAGATCATGATGCAGAAGGATTTGGAGAATATTACATCCAAAGAGATAAGAA CAGAGTTGGAAATGCAAATGGTGTGCAACTTGCGGGAATTCAAGGAATTTATAGACAATGAAATGATAGT GATCCTTGGTCAAATGGATAGCCCTACACAGATATTTGAGCATGTGTTCCTGGGCTCAGAATGGAATGCC TCCAACTTAGAGGACTTACAGAACCGAGGGGTACGGTATATCTTGAATGTCACTCGAGAGATAGATAACT TCTTCCCAGGAGTCTTTGAGTATCATAACATTCGGGTATATGATGAAGAGGCAACGGATCTCCTGGCGTA CTGGAATGACACTTACAAATTCATCTCTAAAGCAAAGAAACATGGATCTAAATGCCTTGTGCACTGCAAA ATGGGGGTGAGTCGCTCAGCCTCCACCGTGATTGCCTATGCAATGAAGGAATATGGCTGGAATCTGGACC GAGCCTATGACTATGTGAAAGAAAGACGAACGGTAACCAAGCCCAACCCAAGCTTCATGAGACAACTGGA AGAGTATCAGGGGATCTTGCTGGCAAGCAAACAGCGGCATAACAAACTATGGAGATCTCATTCAGATAGT GACCTCTCAGACCACCACGAACCCATCTGCAAACCTGGGCTAGAACTCAACAAGAAGGATATCACCACCT CAGCAGACCAGATTGCTGAGGTGAAGACCATGGAGAGTCACCCACCCATACCTCCTGTCTTTGTGGAACA TATGGTCCCACAAGATGCAAATCAGAAAGGCCTGTGTACCAAAGAAAGAATGATCTGCTTGGAGTTTACT TCTAGGGAATTTCATGCTGGACAGATTGAGGATGAATTAAACTTAAATGACATCAATGGATGCTCATCAG GGTGTTGTCTGAATGAATCAAAATTTCCTCTTGACAATTGCCATGCATCCAAAGCCTTAATTCAGCCTGG ACATGTCCCAGAAATGGCCAACAAGTTTCCAGACTTAACAGTGGAAGATTTGGAGACAGATGCACTGAAA GCAGACATGAATGTCCACCTACTGCCTATGGAAGAATTGACATCTCCACTGAAAGACCCCCCCATGTCCC CTGATCCTGAGTCACCAAGCCCCCAACCCAGTTGCCAGACTGAAATCTCAGATTTCAGTACAGATCGCAT TGACTTTTTTAGTGCCCTAGAGAAGTTTGTGGAGCTCTCCCAAGAAACCCGGTCACGATCTTTTTCCCAT TCAAGGATGGAGGAACTGGGTGGAGGAAGGAATGAGAGCTGTCGACTGTCAGTGGTAGAAGTAGCCCCTT CCAAAGTGACAGCTGATGACCAGAGAAGCAGCTCTTTGAGTAATACTCCCCATGCATCAGAAGAATCTTC AATGGATGAGGAACAGTCAAAGGCAATTTCAGAACTGGTCAGCCCAGACATCTTCATGCAGTCTCACTCG GAAAATGCAATTTCAGTCAAAGAAATTGTCACTGAAATTGAGTCCATCAGTCAAGGAGTTGGGCAGATTC AACTGAAAGGAGACATCTTACCCAACCCATGCCATACACCAAAGAAGAACAGCATCCATGAGCTGCTCCT TGAGAGGGCCCAGACTCCAGAGAACAAACCTGGACATATGGAGCAAGATGAGGACTCCTGCACAGCCCAG CCTGAACTAGCCAAAGACTCAGGGATGTGCAACCCAGAAGGCTGCCTAACCACACACTCATCTATAGCAG ACTTGGAAGAAGGGGAACCAGCTGAGGGGGAACAAGAGCTCCAGGGCTCAGGGATGCACCCAGGTGCCAA GTGGTACCCTGGGTCTGTGAGGCGAGCCACCTTGGAGTTCGAAGAGCGCTTACGGCAGGAGCAAGAGCAT CATGGTGCTGCCCCAACATGTACCTCATTGTCCACTCGTAAGAATTCAAAGAATGATTCTTCTGTGGCAG ACCTAGCACCAAAAGGGAAAAGTGATGAAGCCCCCCCAGAACATTCATTTGTCCTCAAGGAACCAGAAAT GAGCAAAGGCAAAGGGAAATACAGTGGGTCTGAGGCTGGCTCACTGTCCCATTCTGAGCAGAATGCCACT GTTCCAGCTCCCAGGGTGCTGGAGTTTGACCACTTGCCAGATCCTCAGGAGGGCCCAGGGTCAGATACTG GAACACAGCAGGAAGGAGTCCTGAAGGATCTGAGGACTGTGATTCCATACCAGGAGTCTGAAACACAAGC AGTCCCTCTTCCCCTTCCCAAGAGGGTAGAAATCATTGAATATACCCACATAGTTACATCACCCAATCAC ACTGGGCCAGGGAGTGAAATAGCCACCAGTGAGAAGAGCGGAGAGCAAGGGCTGAGGAAAGTGAACATGG AAAAATCTGTCACTGTGCTCTGCACACTGGATGAAAATCTAAACAGGACTCTGGACCCCAACCAGGTTTC TCTGCACCCCCAAGTGCTACCTCTGCCTCATTCTTCCTCCCCTGAGCACAACAGACCCACTGACCATCCA ACCTCCATCCTGAGTAGCCCTGAAGACAGAGGCAGCAGCCTGTCCACAGCCCTGGAGACAGCAGCACCTT TTGTCAGTCATACAACCCATTTACTGTCTGCCAGTTTGGATTACCTGCATCCCCAGACTATGGTTCACCT GGAGGGCTTCACAGAGCAGAGCAGCACTACAGATGAGCCCTCTGCAGAACAGGTTAGCTGGGAAGAAAGT CAGGAGAGCCCTCTCTCCAGTGGCAGTGAGGTGCCATATAAGGACTCCCAGCTAAGTAGCGCAGACCTAA GTTTAATTAGCAAACTTGGTGACAACACTGGGGAGTTACAGGAGAAAATGGACCCATTGCCTGTAGCCTG TCGACTCCCACATAGCTCTAGTAGTGAAAACATAAAGAGTCTCAGCCACAGCCCCGGTGTGGTGAAGGAG CGTGCTAAAGAAATCGAGTCTCGAGTGGTTTTCCAGGCAGGGCTCACCAAACCATCCCAAATGAGGCGCT CAGCTTCTCTCGCCAAATTAGGTTACTTGGACCTCTGTAAAGACTGCTTACCAGAGAGGGAGCCTGCCTC CTGTGAATCCCCTCATCTCAAACTGCTTCAGCCTTTCCTCAGAACAGACTCAGGCATGCACGCGATGGAG GACCAAGAGTCCCTAGAAAACCCAGGTGCCCCCCACAACCCAGAGCCCACCAAGTCTTTTGTAGAACAAC TCACAACAACAGAGTGTATTGTGCAGAGCAAGCCAGTGGAGAGGCCCCTTGTGCAGTATGCCAAAGAATT TGGTTCTAGTCAGCAGTATTTGCTCCCCAGGGCAGGACTTGAATTGACTAGTTCTGAAGGAGGCCTTCCC GTGCTACAGACCCAGGGACTGCAGTGTGCATGCCCAGCTCCAGGGCTGGCCGTGGCACCCCGTCAGCAAC ACGGCAGAACTCACCCCCTTAGGAGACTGAAAAAGGCAAATGACAAAAAACGGACAACCAACCCCTTCTA TAATACCATGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001282129
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282129.1, NP_001269058.1
RefSeq Size	9493 bp
RefSeq ORF	4353 bp
Locus ID	85464
UniProt ID	Q76I76
Protein Families	Druggable Genome, Phosphatase
Protein Pathways	Regulation of actin cytoskeleton
Gene Summary	This gene encodes a protein tyrosine phosphatase that plays a key role in the regulation of actin filaments. The encoded protein dephosphorylates and activates cofilin, which promotes actin filament depolymerization. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.