ATP7A (NM_001282224) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DSMAX; MK; MNK; SMAX3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001282224, the custom clone sequence may differ by one or more nucleotides ATGGATCCAAGTATGGGTGTGAATTCTGTTACCATTTCTGTTGAGGGTATGACTTGCAATTCCTGTGTTT GGACCATTGAGCAGCAGATTGGAAAAGTGAATGGTGTGCATCACATTAAGGTATCACTGGAAGAAAAAAA TGCAACTATTATTTATGACCCTAAACTACAGACTCCAAAGACCCTACAGGAAGCTATTGATGACATGGGC TTTGATGCTGTTATCCATAATCCTGACCCTCTCCCTGTTTTAACTGACACCTTGTTTCTGACTGTTACGG CGTCACTGACTTTGCCATGGGACCATATCCAAAGCACATTGCTGAAGACCAAGGGTGTGACAGACATTAA AATTTACCCTCAGAAAAGAACTGTAGCAGTGACAATAATCCCTTCTATAGTGAATGCCAATCAGATAAAA GAGCTGGTTCCAGAACTCAGTTTAGATACTGGGACACTGGAGAAAAAGTCAGGAGCTTGTGAAGATCATA GTATGGCTCAAGCTGGTGAAGTCGTGCTGAAGATGAAAGTGGAAGGGATGACCTGCCATTCATGTACTAG CACTATTGAAGGAAAAATTGGGAAACTGCAAGGTGTTCAGCGAATTAAAGTCTCCCTGGACAATCAAGAA GCTACTATTGTTTATCAACCTCATCTTATCTCAGTAGAGGAAATGAAAAAGCAGATTGAAGCTATGGGCT TTCCAGCATTTGTCAAAAAGCAGCCCAAGTACCTCAAATTGGGAGCTATTGATGTAGAACGTCTAAAGAA CACACCAGTTAAATCCTCAGAAGGGTCACAGCAAAGGAGTCCATCATATACCAATGATTCAACAGCCACT TTCATCATTGATGGCATGCATTGTAAATCATGTGTGTCAAATATTGAAAGTACTTTATCTGCACTCCAAT ATGTAAGCAGCATAGTAGTTTCTTTAGAGAATAGGTCTGCCATTGTGAAGTATAATGCAAGCTCAGTCAC TCCAGAATCCCTGAGAAAAGCAATAGAGGCTGTATCACCGGGGCTATATAGAGTTAGTATCACAAGTGAA GTTGAGAGTACCTCAAACTCTCCCTCCAGCTCATCTCTTCAGAAGATTCCTTTGAATGTAGTTAGCCAGC CTCTGACACAAGAAACTGTGATAAACATTGATGGCATGACTTGTAATTCCTGTGTGCAGTCTATTGAGGG TGTCATATCAAAAAAGCCAGGTGTAAAATCCATACGAGTCTCCCTTGCAAATAGCAATGGGACTGTTGAG TATGATCCTCTACTAACCTCTCCAGAAACGTTGAGAGGAGCAATAGAAGACATGGGATTTGATGCTACCT TGTCAGACACGAATGAGCCGTTGGTAGTAATAGCTCAGCCTTCATCGGAAATGCCGCTTTTGACTTCAAC TAATGAATTTTATACTAAAGGGATGACACCAGTTCAAGACAAGGAGGAAGGAAAGAATTCATCTAAGTGT TACATACAGGTCACTGGCATGACTTGCGCTTCCTGTGTAGCAAACATTGAACGGAATTTAAGGCGGGAAG AAGGAATATATTCTATACTTGTGGCCCTGATGGCTGGCAAGGCAGAAGTAAGGTATAATCCTGCTGTTAT ACAACCCCCAATGATAGCAGAGTTCATCCGAGAACTTGGATTTGGAGCCACTGTGATAGAAAATGCTGAT GAAGGAGATGGTGTTTTGGAACTTGTTGTGAGGGGAATGACGTGTGCCTCCTGCGTACATAAAATAGAGT CTAGTCTCACAAAACACAGAGGGATCCTATACTGCTCCGTGGCCCTGGCAACCAACAAAGCACATATTAA ATATGACCCAGAAATTATTGGTCCTAGAGATATTATCCATACAATTGAAAGCTTAGGTTTTGAAGCTTCT TTGGTCAAGAAGGATCGGTCAGCAAGTCACTTAGATCATAAACGAGAAATAAGACAATGGAGACGGTCTT TTCTTGTGAGTCTGTTTTTCTGTATTCCTGTAATGGGGCTGATGATATATATGATGGTTATGGACCACCA CTTTGCAACTCTTCACCATAATCAAAACATGAGTAAAGAAGAAATGATCAACCTTCATTCTTCTATGTTC CTGGAGCGCCAGATTCTTCCAGGATTGTCTGTTATGAATTTGCTGTCCTTTTTATTGTGTGTACCTGTAC AGGGCAAAACATCAGAGGCTCTTGCAAAGTTAATTTCACTACAAGCTACAGAAGCAACTATTGTAACTCT TGATTCTGATAATATCCTCCTCAGTGAAGAACAAGTGGATGTGGAACTTGTACAACGTGGAGATATCATT AAAGTAGTTCCAGGAGGCAAATTTCCAGTGGATGGTCGTGTTATTGAAGGACATTCTATGGTAGATGAGT CCCTCATCACAGGGGAGGCAATGCCTGTGGCTAAGAAACCTGGCAGCACAGTGATTGCTGGTTCCATTAA CCAGAACGGGTCACTGCTTATCTGCGCAACACATGTTGGAGCAGACACAACCCTTTCTCAAATTGTCAAA CTTGTGGAAGAGGCACAAACATCAAAGGCTCCTATCCAGCAGTTTGCAGACAAACTCAGTGGCTATTTTG TTCCTTTTATTGTTTTTGTTTCCATTGCCACCCTCTTGGTATGGATTGTAATTGGATTTCTGAATTTTGA AATTGTGGAAACCTACTTTCCTGGCTACAATAGAAGTATCTCCCGAACAGAAACGATAATACGATTTGCT TTCCAAGCCTCTATCACAGTTCTGTGTATTGCATGTCCCTGTTCACTGGGACTGGCCACTCCAACTGCTG TGATGGTGGGTACAGGAGTAGGTGCTCAAAATGGCATACTAATAAAAGGTGGAGAGCCATTGGAGATGGC TCATAAGGTAAAGGTAGTGGTATTTGATAAGACTGGAACCATTACTCACGGAACCCCAGTGGTGAATCAA GTAAAGGTTCTAACTGAAAGTAACAGAATATCACACCATAAAATCTTGGCCATTGTGGGAACTGCTGAAA GTAACAGTGAACACCCTCTAGGAACAGCCATAACCAAATATTGCAAACAGGAGCTGGACACTGAAACCTT GGGTACCTGCATAGATTTCCAGGTTGTGCCAGGCTGTGGTATTAGCTGTAAAGTCACCAATATTGAAGGC TTGCTACATAAGAATAACTGGAATATAGAGGACAATAATATTAAAAATGCATCCCTGGTTCAAATTGATG CCAGTAATGAACAGTCATCAACTTCGTCTTCCATGATTATTGATGCCCAGATCTCAAATGCTCTTAATGC TCAGCAGTATAAAGTCCTCATTGGTAACCGGGAGTGGATGATTAGAAATGGTCTTGTCATTAATAACGAT GTAAATGATTTCATGACTGAACATGAGAGAAAAGGTCGGACTGCTGTATTAGTAGCAGTTGATGATGAGC TGTGTGGCTTGATAGCCATTGCAGACACAGTGAAGCCTGAAGCAGAACTGGCTATCCATATTCTGAAATC TATGGGCTTAGAAGTAGTTCTGATGACTGGAGACAACAGTAAAACAGCTAGATCTATTGCTTCTCAGGTT GGCATTACTAAGGTGTTTGCTGAAGTTCTACCTTCTCACAAGGTTGCTAAAGTGAAGCAACTTCAAGAGG AGGGGAAACGGGTAGCAATGGTGGGAGATGGAATCAATGACTCCCCAGCTCTGGCAATGGCTAATGTGGG AATTGCTATTGGCACAGGCACAGATGTAGCCATTGAAGCAGCTGATGTGGTTTTGATAAGGAATGATCTT CTGGATGTAGTGGCAAGTATTGACTTATCAAGAAAGACAGTCAAGAGGATTCGGATAAATTTTGTCTTTG CTCTAATTTATAATCTGGTTGGAATTCCCATAGCTGCTGGAGTTTTTATGCCCATTGGTTTGGTTTTGCA GCCCTGGATGGGATCTGCAGCAATGGCTGCTTCATCTGTTTCTGTAGTACTTTCTTCTCTCTTCCTTAAA CTTTACAGGAAACCAACTTACGAGAGTTATGAACTGCCTGCCCGGAGCCAGATAGGACAGAAGAGTCCTT CAGAAATCAGCGTTCATGTTGGAATAGATGATACCTCAAGGAATTCTCCTAAACTGGGTTTGCTGGACCG GATTGTTAATTATAGCAGAGCCTCTATAAACTCACTACTGTCTGATAAACGCTCCCTAAACAGTGTTGTT ACCAGTGAACCTGACAAGCACTCACTCCTGGTGGGAGACTTCAGGGAAGATGATGACACTGCATTATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001282224
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282224.1, NP_001269153.1
RefSeq Size	8306 bp
RefSeq ORF	4269 bp
Locus ID	538
UniProt ID	Q04656
Protein Families	Druggable Genome, Transmembrane
Gene Summary	This gene encodes a transmembrane protein that functions in copper transport across membranes. This protein is localized to the trans Golgi network, where it is predicted to supply copper to copper-dependent enzymes in the secretory pathway. It relocalizes to the plasma membrane under conditions of elevated extracellular copper, and functions in the efflux of copper from cells. Mutations in this gene are associated with Menkes disease, X-linked distal spinal muscular atrophy, and occipital horn syndrome. Alternatively-spliced transcript variants have been observed. [provided by RefSeq, Aug 2013] Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. This variant is supported by data in PMIDs 7490081 and 10970802. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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