FMN1 (NM_001277313) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FMN; LD
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001277313, the custom clone sequence may differ by one or more nucleotides ATGGAAGGCACTCATTGTACCCTCCAATTGCATAAGCCCATTACGGAACTCTGCTACATCAGCTTCTGTC TTCCAAAGGGGGAAGTCAGAGGATTTTCATACAAGGGCACTGTAACTCTAGACAGATCCAATAAAGGTTT TCATAACTGCTACCAAGTCAGGGAGGAGTCAGACATCATCAGCCTCAGCCAGGAGCCGGACGAACATCCA GGCGACATATTTTTCAAGCAGACTCCCACGAAAGACATTCTAACTGAGCTGTACAAACTCACAACAGAGA GGGAGAGACTGCTAACCAATCTCCTGAGCTCAGACCACATCCTGGGGATCACGATGGGGAACCAGGAGGG GAAGCTGCAAGAGCTGTCCGTGAGCCTGGCCCCCGAGGATGACTGTTTCCAGAGTGCTGGTGACTGGCAG GGAGAGCTCCCCGTGGGCCCTCTCAATAAGAGGAGCACCCACGGGAACAAAAAGCCTCGGAGGTCTAGTG GAAGGAGAGAGAGCTTTGGGGCCCTTCCACAGAAGAGGACCAAAAGAAAAGGGCGTGGAGGCCGAGAATC AGCTCCTCTGATGGGCAAGGACAAGATCTGTTCCAGCCACTCCCTTCCTCTTTCTAGAACAAGGCCTAAC CTTTGGGTACTAGAGGAGAAAGGAAATCTGCTCCCGAATGGGGCACTTGCCTGCTCCCTGCAGAGGAGAG AGAGCTGCCCCCCAGATATTCCCAAGACGCCAGACACAGACCTTGGCTTTGGGAGCTTTGAGACGGCTTT CAAGGACACTGGGCTTGGAAGAGAAGTGCTGCCCCCTGACTGCAGCTCCACAGAGGCAGGAGGGGATGGC ATTCGGAGGCCGCCGAGCGGGCTGGAGCATCAGCAAACAGGTTTGTCTGAAAGTCACCAGGACCCTGAGA AGCATCCAGAGGCAGAAAAGGATGAGATGGAGAAGCCGGCTAAGCGGACTTGCAAGCAGAAACCTGTCTC CAAAGTGGTGGCCAAAGTTCAGGACCTGTCCTCCCAGGTACAAAGAGTAGTTAAAACGCATTCTAAGGGT AAGGAGACGATTGCCATTCGCCCAGCAGCCCACGCTGAGTTTGTACCCAAAGCCGACTTGCTCACCCTCC CGGGAGCTGAGGCTGGGGCTCATGGCTCCAGGCGGCAGGGCAAGGAGCGGCAAGGGGATAGGTCATCGCA GTCGCCAGCCGGGGAAACAGCCTCCATTTCTAGTGTGTCGGCCAGTGCCGAGGGGGCCGTGAACAAGGTC CCCCTGAAGGTGATAGAGAGTGAGAAGTTAGATGAAGCCCCTGAGGGGAAAAGACTGGGCTTCCCTGTCC ACACGAGTGTCCCTCACACTCGCCCAGAAACGAGAAACAAGAGGAGAGCCGGGTTGCCCCTTGGTGGCCA CAAGTCCTTGTTTCTGGATCTGCCCCACAAAGTAGGTCCTGACTCCTCACAACCCAGAGGTGATAAGAAG AAGCCATCCCCACCAGCACCGGCAGCTCTTGGCAAGGTGTTTAATAATTCAGCCTCGCAGTCCAGCACAC ACAAACAGACGTCACCTGTTCCCTCGCCTCTGTCTCCAAGGCTCCCCAGCCCTCAGCAGCATCACAGGAT CCTCCGGCTCCCTGCATTGCCTGGTGAGAGGGAAGCTGCTCTTAATGACTCTCCTTGTAGAAAGAGCCGT GTCTTCTCTGGGTGCGTCTCTGCTGACACCTTGGAGCCACCATCCTCTGCAAAGGTCACGGAGACCAAAG GAGCCAGCCCGGCCTTCCTCAGAGCAGGCCAACCTCGGTTGGTGCCTGGGGAAACTTTGGAAAAGAGCTT GGGGCCAGGGAAGACCACAGCTGAGCCCCAGCACCAGTCACCTCCAGGTATCTCCTCTGAGGGCTTTCCC TGGGACGGCTTCAATGAGCAGACACCTAAAGACCTTCCCAACAGAGATGGAGGCGCGTGGGTTCTGGGCT ACAGAGCGGGACCAGCCTGTCCATTTTTGCTTCATGAGGAAAGGGAGAAGTCAAACAGGAGCGAATTGTA CTTGGATCTCCATCCTGACCACAGCCTGACTGAGCAGGATGACAGGACTCCTGGCAGACTTCAAGCTGTC TGGCCACCCCCAAAGACAAAAGACACAGAAGAAAAAGTGGGACTGAAGTACACTGAAGCAGAATACCAAG CTGCTATTTTACACTTGAAGAGGGAGCACAAAGAAGAAATTGAAAACCTGCAGGCACAGTTTGAACTTCG GGCATTTCATATCCGGGGCGAGCATGCAATGATAACAGCGAGACTAGAAGAAACCATTGAAAATCTGAAA CACGAGCTAGAACACAGATGGCGAGGGGGTTGTGAAGAGAGGAAAGATGTGTGCATTTCCACCGATGATG ACTGCCCTCCAAAGACCTTCAGAAATGTGTGCGTCCAGACAGACAGAGAGACCTTCCTCAAGCCCTGTGA AAGTGAAAGCAAGACAACCAGAAGTAATCAATTAGTACCCAAAAAGCTGAATATCTCCTCTTTAAGCCAG CTCTCACCCCCAAATGACCACAAAGACATCCATGCAGCACTCCAGCCAATGGAGGGCATGGCATCAAATC AGCAGAAGGCATTGCCTCCGCCTCCCGCATCCATCCCTCCCCCTCCGCCCCTCCCCTCAGGACTTGGATC TTTGTCTCCCGCACCTCCAATGCCACCTGTGAGTGCTGGGCCACCGCTACCACCTCCGCCTCCTCCACCG CCACCTCTACCTCCACCTTCAAGTGCTGGACCTCCACCACCCCCACCCCCACCCCCACTTCCTAACTCCC CTGCCCCACCCAACCCTGGAGGGCCTCCTCCTGCTCCACCACCCCCAGGACTTGCACCCCCACCTCCCCC TGGACTCTTCTTTGGACTTGGCTCTTCTTCCAGTCAATGTCCTCGAAAACCAGCCATCGAGCCCAGTTGT CCCATGAAGCCTTTATATTGGACTAGGATACAAATAAGTGATAGGAGCCAAAATGCTACACCAACCTTAT GGGACTCCTTAGAAGAACCTGACATTCGGGACCCCAGTGAATTTGAGTATTTATTCTCCAAAGACACAAC TCAACAGAAGAAAAAACCTCTGTCAGAGACTTATGAGAAGAAAAACAAGGTCAAAAAGATCATCAAATTG TTGGATGGAAAACGATCTCAAACTGTGGGAATCTTGATATCTAGTTTACATTTAGAAATGAAGGATATCC AACAGGCCATTTTCAATGTGGATGACTCCGTGGTTGATCTGGAGACCCTGGCAGCCTTATATGAAAACAG AGCCCAAGAGGATGAGCTGGTTAAAATAAGAAAGTATTACGAGACATCCAAAGAAGAAGAACTGAAGCTG CTGGATAAACCTGAGCAATTTTTACATGAGTTAGCCCAGATTCCTAATTTTGCTGAACGTGCCCAGTGCA TAATCTTCAGATCTGTCTTTTCTGAGGGTATCACCTCCTTGCACAGAAAGGTAGAGATCATCACGCGAGC TTCTAAGGACTTGCTGCACGTGAAGAGCGTGAAGGATATTTTAGCTCTCATCTTGGCTTTTGGAAATTAT ATGAATGGAGGAAATAGGACTCGGGGACAAGCCGATGGATATAGCTTAGAAATTCTGCCCAAACTCAAGG ATGTCAAAAGTCGGGATAATGGGATTAATCTGGTGGACTACGTTGTTAAGTATTACCTGCGTTACTATGA TCAGGAAGCTGGAACAGAAAAGAGTGTTTTCCCCTTGCCGGAACCACAGGATTTCTTTCTGGCCTCCCAA GTCAAGTTTGAAGACCTCATAAAAGATTTGAGAAAACTGAAGAGGCAACTAGAAGCAAGTGAGAAACAGA TGGTGGTGGTGTGCAAGGAGTCCCCAAAGGAGTATCTCCAGCCTTTCAAGGACAAACTAGAGGAGTTCTT CCAAAAAGCCAAAAAAGAGCATAAGATGGAAGAAAGTCACTTGGAGAATGCACAGAAAAGTTTTGAAACA ACAGTACGATATTTTGGGATGAAGCCAAAGTCTGGTGAGAAGGAGATCACACCCAGCTACGTGTTTATGG TGTGGTATGAGTTCTGCAGTGACTTCAAGACAATTTGGAAACGGGAGAGTAAAAACATATCTAAAGAAAG ATTGAAAATGGCTCAGGAATCAGTCAGCAAGTTGACTTCAGAGAAGAAAGTGGAGACAAAGAAAATCAAT CCCACTGCTAGCCTGAAAGAAAGACTGCGTCAGAAGGAAGCCAGTGTGACCACTAACTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001277313
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001277313.1, NP_001264242.1
RefSeq Size	13546 bp
RefSeq ORF	4260 bp
Locus ID	342184
UniProt ID	Q68DA7
Gene Summary	This gene belongs to the formin homology family and encodes a protein that has a role in the formation of adherens junction and the polymerization of linear actin cables. The homologous gene in mouse is associated with limb deformity. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). The exon combination of this variant is inferred based on partial human and full-length orthologous transcript alignments. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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