PPRC1 (NM_001288727) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PRC
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001288727, the custom clone sequence may differ by one or more nucleotides ATGGCGGCGCGCCGGGGACGGAGAGACGGAGTCGCGCCGCCCCCGAGTGGGGGCCCCGGTCCGGACCCTG GCGGGGGAGCCCGCGGCAGTGGTTGGGGAAGTCGAAGCCAAGCGCCGTATGGGACTTTGGGCGCTGTGAG CGGCGGCGAGCAGGTGCTGCTGCATGAGGAGGCGGGTGATTCTGGCTTTGTCAGTCTCTCTCGGCTGGGC CCATCTCTGAGGGACAAGGACCTGGAAATGGAGGAGCTAATGCTGCAGGATGAGACACTGCTGGGGACCA TGCAGAGCTACATGGATGCCTCCCTTATCTCCCTCATTGAGGATTTTGGGAGCCTTGGAGAGAGCAGGTT ATCTCTGGAGGACCAGAATGAAGTGTCGCTGCTCACGGCTCTGACGGAGATCTTGGACAATGCAGATTCT GAGAACCTTTCTCCATTTGACAGCATTCCTGATTCGGAGCTGCTTGTGTCACCCCGGGAGGGCTCCTCTC TGCACAAGCTGCTTACTCTCTCTCGGACACCCCCAGAACGTGACCTCATCACCCCAGTTGACCCACTGGG GCCCAGTACAGGCAGCAGTAGAGGGAGTGGGGTTGAAATGTCTCTTCCAGATCCCTCTTGGGACTTCTCC CCACCCTCTTTCTTAGAGACCTCTTCCCCCAAGCTTCCTAGCTGGAGACCCCCAAGATCAAGACCACGCT GGGGCCAATCCCCACCTCCCCAGCAGCGCAGTGATGGAGAAGAAGAGGAGGAGGTGGCCAGCTTCAGTGG CCAGATTCTTGCCGGGGAGCTTGACAACTGTGTGAGCAGTATCCCGGACTTCCCCATGCATTTGGCCTGC CCTGAGGAGGAAGATAAAGCAACAGCAGCAGAGATGGCAGTGCCAGCAGCTGGTGATGAGAGCATCTCCT CCCTGAGTGAGCTGGTGCGGGCCATGCACCCATACTGCCTGCCCAACCTCACCCACCTGGCATCACTTGA GGATGAGCTTCAGGAGCAGCCAGATGATTTGACACTGCCTGAGGGCTGCGTAGTGCTGGAGATTGTGGGG CAGGCAGCCACAGCTGGCGATGACCTGGAGATCCCAGTTGTGGTGCGACAGGTCTCTCCTGGACCCCGGC CTGTGCTCCTGGATGACTCGCTAGAGACTAGTTCTGCCTTGCAGCTGCTTATGCCTACACTGGAGTCAGA GACAGAGGCTGCTGTGCCCAAGGTAACCCTCTGCTCTGAGAAAGAGGGGTTGTCATTGAACTCAGAGGAG AAGCTGGACTCAGCCTGCTTATTGAAGCCCAGGGAGGTCGTGGAGCCGGTGGTGCCCAAGGAGCCTCAGA ACCCACCTGCCAATGCAGCACCAGGTTCCCAGAGAGCTCGAAAGGGCAGGAAGAAGAAGAGCAAGGAGCA GCCAGCAGCCTGTGTGGAAGGCTATGCCAGGAGGCTGAGGTCATCTTCTCGCGGGCAGTCTACTGTAGGT ACAGAAGTGACCTCTCAGGTAGACAACTTGCAGAAACAGCCTCAGGAAGAACTTCAAAAAGAGTCTGGGC CTCTCCAGGGTAAGGGGAAGCCCCGGGCTTGGGCTCGGGCCTGGGCAGCTGCCTTGGAGAATTCTAGCCC TAAGAACTTGGAGAGAAGTGCTGGACAAAGTAGTCCTGCTAAAGAAGGCCCTCTAGACCTCTACCCAAAG CTGGCTGACACTATCCAAACCAATCCTATACCAACCCATCTCTCATTGGTCGACTCTGCCCAAGCCAGCC CCATGCCAGTTGACTCTGTTGAAGCTGATCCCACTGCAGTTGGCCCTGTTCTAGCTGGCCCTGTACCTGT TGACCCTGGGTTGGTTGACCTTGCTTCAACCAGCTCAGAACTGGTTGAGCCTCTCCCGGCTGAGCCAGTG CTGATCAACCCAGTCCTGGCTGACTCAGCAGCAGTTGACCCTGCAGTGGTTCCCATCTCAGATAACTTGC CACCAGTTGATGCTGTCCCGTCTGGCCCAGCACCAGTTGATCTAGCACTGGTTGACCCTGTTCCTAATGA CCTGACTCCAGTTGACCCAGTGCTAGTTAAGTCCAGACCAACTGATCCCAGACGTGGTGCAGTGTCATCA GCCCTGGGGGGTTCAGCACCCCAGCTCCTCGTGGAGTCAGAGTCCTTGGACCCACCAAAGACCATCATCC CTGAAGTCAAAGAGGTTGTGGATTCTCTGAAAATTGAAAGTGGTACCAGTGCTACAACCCATGAAGCCAG ACCTCGGCCTCTCAGCTTATCTGAGTACCGGCGACGAAGGCAGCAACGCCAAGCAGAAACAGAAGAGAGA AGTCCACAGCCCCCAACTGGGAAGTGGCCTAGCCTTCCAGAGACTCCCACAGGGCTGGCAGACATCCCTT GTCTTGTCATCCCACCAGCCCCAGCCAAGAAGACAGCTCTGCAGAGAAGCCCTGAAACACCCCTTGAGAT TTGCCTTGTGCCTGTAGGTCCCAGCCCTGCTTCTCCTAGTCCTGAGCCACCTGTAAGCAAACCTGTGGCC TCATCTCCCACTGAGCAGGTGCCATCCCAGGAGATGCCACTGTTGGCGAGACCTTCCCCTCCTGTGCAGT CTGTGTCCCCTGCTGTGCCCACACCTCCCTCGATGTCTGCTGCCCTGCCTTTCCCTGCAGGTGGGCTTGG CATGCCCCCCAGTCTGCCCCCACCTCCCTTGCAGCCTCCTAGTCTTCCATTGTCTATGGGGCCAGTACTA CCTGATCCGTTTACTCACTATGCCCCCTTGCCATCCTGGCCTTGTTATCCTCATGTGTCCCCTTCTGGCT ATCCTTGCCTGCCCCCCCCACCAACGGTGCCCCTAGTGTCTGGTACTCCTGGTGCCTATGCCGTGCCTCC CACTTGCAGTGTGCCTTGGGCACCCCCTCCTGCCCCAGTCTCACCTTACAGTTCCACATGTACCTATGGG CCCTTGGGATGGGGCCCAGGGCCTCAACATGCTCCATTCTGGTCTACTGTTCCCCCACCTCCTTTGCCTC CAGCCTCCATTGGGAGAGCTGTTCCCCAACCTAAAATGGAGTCTAGGGGCACTCCAGCTGGCCCTCCTGA AAATGTACTTCCCTTGTCGATGGCTCCTCCCCTCAGTCTTGGGCTACCTGGCCATGGAGCTCCTCAGACA GAGCCTACCAAGGTGGAGGTCAAGCCAGTGCCTGCATCTCCCCATCCGAAACACAAGGTGTCTGCCCTGG TGCAAAGTCCCCAGATGAAGGCTCTAGCATGTGTGTCTGCTGAAGGTGTGACTGTTGAGGAGCCTGCATC AGAGAGGCTAAAGCCTGAGACCCAAGAGACCAGGCCCAGGGAGAAGCCCCCCTTGCCTGCTACCAAGGCT GTTCCCACACCAAGGCAGAGCACTGTCCCCAAGCTGCCTGCTGTCCACCCAGCCCGTCTAAGGAAGCTGT CCTTCCTGCCTACCCCACGTACTCAGGGTTCTGAAGATGTGGTACAGGCTTTCATCAGTGAGATTGGAAT TGAGGCATCGGACCTGTCCAGTCTGCTGGAGCAGTTTGAGAAATCAGAAGCCAAAAAGGAGTGTCCTCCT CCGGCTCCTGCTGACAGCTTGGCTGTAGGAAACTCAGGGTCCAGCTGTAGTTCCTCTGGACGTTCTCGAA GATGCTCTTCCTCTTCTTCGTCATCATCTTCCTCTTCGTCTTCCTCATCCTCATCATCCAGTTCTCGAAG CCGCTCACGATCCCCATCCCCCCGCCGGAGAAGTGACAGGAGGCGGCGGTACAGCTCTTATCGTTCACAT GACCATTACCAAAGGCAAAGAGTGCTACAAAAGGAGCGTGCAATAGAAGAAAGAAGGGTGGTCTTCATTG GAAAGATACCTGGCCGCATGACTCGATCAGAGCTGAAACAGAGGTTCTCCGTTTTTGGAGAGATTGAGGA GTGCACCATCCACTTCCGTGTCCAAGGGGACAACTACGGCTTCGTCACTTATCGCTATGCTGAGGAGGCA TTTGCAGCCATTGAGAGTGGCCACAAGCTGCGGCAGGCAGATGAGCAGCCCTTTGATCTCTGCTTTGGGG GCCGAAGGCAGTTCTGCAAGAGGAGCTATTCTGATCTTGACTCCAACCGGGAAGACTTTGACCCAGCACC TGTAAAGAGCAAATTTGATTCTCTTGACTTTGACACATTGTTGAAACAGGCCCAGAAGAACCTCAGGAGG TAA
Restriction Sites	SgfI-MluI
ACCN	NM_001288727
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001288727.1, NP_001275656.1
RefSeq Size	4598 bp
RefSeq ORF	4203 bp
Locus ID	23082
UniProt ID	Q5VV67
Protein Families	Druggable Genome
Gene Summary	The protein encoded by this gene is similar to PPAR-gamma coactivator 1 (PPARGC1/PGC-1), a protein that can activate mitochondrial biogenesis in part through a direct interaction with nuclear respiratory factor 1 (NRF1). This protein has been shown to interact with NRF1. It is thought to be a functional relative of PPAR-gamma coactivator 1 that activates mitochondrial biogenesis through NRF1 in response to proliferative signals. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013] Transcript Variant: This variant (2) lacks two exons in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.

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