IBTK (NM_001300906) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BTBD26; BTKI
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001300906, the custom clone sequence may differ by one or more nucleotides ATGAGTTCACCCATGCCTGACTGCACATCAAAGTGTCGATCCCTGAAGCATGCTTTGGATGTCCTTTCTG TGGTAACAAAGGGGAGCGAAAACCAGATTAAGGCCTTTCTCTCCAGTCATTGTTACAATGCTGCAACTAT CAAGGATGTTTTTGGCAGGAATGCCCTCCACCTTGTTTCCTCCTGTGGAAAGAAAGGAGTGTTAGATTGG CTTATTCAGAAAGGAGTGGATCTGTTGGTGAAAGACAAAGAGTCTGGATGGACAGCATTGCACAGAAGCA TTTTTTATGGACATATTGATTGTGTTTGGTCTCTATTGAAGCATGGTGTTAGTCTGTATATTCAAGATAA AGAAGGCTTGTCAGCTTTGGATCTTGTAATGAAGGATAGACCAACTCATGTAGTATTCAAGAATACTGAT CCTACAGATGTTTATACTTGGGGCGATAATACAAATTTTACCCTGGGTCATGGAAGCCAGAATAGCAAAC ATCATCCAGAGTTGGTGGATCTGTTCTCCAGGAGTGGGATTTATATCAAGCAGGTGGTGCTTTGTAAATT TCACTCCGTGTTTCTGTCTCAGAAAGGGCAGGTTTATACCTGTGGTCATGGTCCTGGAGGGCGATTAGGA CATGGAGATGAACAGACATGCTTGGTCCCTCGGCTTGTGGAAGGACTGAATGGTCATAATTGTTCCCAAG TGGCAGCTGCTAAGGATCATACTGTTGTATTAACTGAAGATGGATGTGTTTATACATTTGGTCTAAACAT TTTTCATCAATTAGGAATTATTCCACCGCCTTCCAGTTGTAATGTACCCAGACAGATACAGGCAAAATAT CTGAAAGGAAGGACAATCATTGGCGTTGCAGCAGGCAGGTTTCATACAGTCCTATGGACTAGAGAAGCTG TTTACACTATGGGACTAAATGGTGGACAACTGGGTTGTTTGCTAGATCCCAATGGAGAAAAGTGTGTAAC TGCTCCTCGTCAGGTCTCTGCCCTTCACCATAAAGACATTGCTCTGTCTTTGGTTGCTGCAAGTGATGGA GCTACAGTCTGTGTTACCACAAGGGGAGATATTTACTTACTTGCAGACTATCAGTGCAAGAAGATGGCTT CTAAACAGTTGAACTTGAAAAAAGTTCTTGTGTCTGGGGGTCATATGGAATACAAGGTTGATCCTGAACA TTTGAAAGAAAATGGGGGTCAAAAAATTTGCATTCTTGCAATGGATGGAGCTGGAAGGGTGTTTTGCTGG AGATCAGTCAACAGTTCTCTGAAGCAGTGTCGATGGGCCTATCCACGTCAGGTCTTCATTTCTGATATTG CTTTAAATAGAAATGAAATTCTATTTGTTACGCAAGATGGAGAAGGATTTAGAGGGAGATGGTTTGAAGA GAAAAGAAAGAGTTCTGAAAAGAAAGAGATTTTATCAAACCTTCACAATTCCTCATCAGATGTGTCTTAT GTCTCTGATATAAATAGTGTGTATGAAAGAATTCGACTTGAGAAACTTACCTTTGCACATAGAGCTGTTA GTGTCAGCACAGATCCAAGTGGATGCAACTTTGCAATCCTGCAGTCAGATCCTAAAACAAGCCTTTATGA AATTCCAGCTGTGTCCTCATCATCCTTTTTTGAAGAGTTTGGCAAACTGTTGAGGGAAGCAGATGAAATG GACAGCATTCATGATGTGACATTTCAAGTTGGCAATAGACTCTTCCCTGCACATAAATATATTTTGGCAG TGCATTCTGATTTTTTTCAGAAATTGTTTCTTTCAGATGGTAATACTTCAGAATTTACAGATATTTACCA GAAAGATGAAGATTCTGCAGGGTGCCATCTCTTTGTGGTAGAGAAGGTTCATCCTGACATGTTTGAATAC CTTTTACAATTTATATACACAGATACTTGTGACTTTTTAACTCATGGCTTCAAACCAAGAATACACTTAA ACAAAAACCCAGAAGAATATCAGGGAACTCTGAATTCTCATTTGAATAAAGTGAATTTCCATGAAGATGA TAACCAGAAGTCTGCATTTGAAGTTTACAAAAGTAATCAAGCTCAAACAGTTAGTGAGAGGCAGAAGAGC AAACCTAAATCTTGTAAAAAAGGAAAAAATATTAGGGAAGATGATCCTGTAAGAATGTTGCAAACTGTTG CAAAGAAATTCGACTTCAGTAATTTGAGTAGTAGGTTAGATGGAGTCAGATTTGAAAATGAAAAAATTAA TGTTATTGCCAAGAACACTGGTAATAAACTGAAGCTAAGTCAGAAAAAATGTTCATTTCTGTGTGACGTG ACCATGAAATCAGTGGATGGAAAGGAATTTCCTTGTCATAAATGTGTTCTTTGTGCTAGACTTGAATATT TTCATAGTATGCTGAGTAGCTCATGGATTGAGGCTTCCAGTTGTGCAGCTCTGGAAATGCCAATACATTC TGATATATTAAAAGTTATTTTGGACTACCTCTATACTGATGAAGCTGTGGTGATAAAAGAATCTCAAAAT GTAGATTTTATTTGTAGTGTTCTTGTGGTGGCTGATCAACTTCTCATAACCCGGTTGAAAGAGATTTGTG AAGTAGCATTAACTGAAAAACTTACCCTGAAGAATGCTGCTATGCTACTGGAATTTGCAGCAATGTATAG TGCAAAACAGTTGAAACTGTCTTGTTTACAGTTTATAGGATTGAATATGGCAGCTTTACTTGAAGCAAGG TCTCTTGATGTTTTAAGCGATGGTGTTTTGAAGGATCTTTCTGAGTTTTACCGGAAAATGATTCCAGCAA TGGATAGAAGAGTCATTACACCATATCAAGATGGACCAGATATTAGCTATTTGGAAGTAGAAGATGGAGA TATCTTCTTGAAAGAAGAAATAAATATGGAACAAAATCATTCGGAAACTATGTTCAAGAAAGCAAAAACA AAAGCTAAAAAGAAGCCACGTAAACGTTCAGATAGTTCTGGAGGTTATAACCTTTCAGATATTATTCAGA GTCCATCATCTACAGAACTGTTGACATCAGACTCTGAAGGAAGCTATGCAGGAGTGGGTAGTCCTAGAGA TTTACAGTCCCCTGATTTCACAACAGGATTTCATTCAGATAAGATTGAGGCGAAAGTCAAACCGTATGTT AATGGTACATCTCCTGTGTATTCTAGGGAAGATTTAAAACCATGGGAAAAGTCACCAATACTTAAAATAT CTGCTCCACAGCCTATTCCCAGTAACAGAATTGATACTACCAGCTCTGCCAGTTGGGTTGCTGGTTCTTT CAGTCCTGTCAGCCCTCCTGTTGTGGATCTCAGAACTATCATGGAAATAGAAGAAAGTAGACAAAAATGT GGAGCTACACCAAAGTCACATTTAGGCAAAACAGTTTCTCATGGAGTTAAACTTTCTCAGAAGCAACGAA AAATGATTGCATTGACTACCAAGGAAAACAATTCAGGAATGAATAGCATGGAAACAGTTTTATTCACTCC TTCAAAAGCCCCCAAACCAGTGAATGCATGGGCATCTTCTCTGCATTCAGTTTCATCCAAGTCATTCCGG GATTTCTTACTAGAAGAAAAAAAGTCTGTTACTAGCCATAGTTCAGGCGATCATGTCAAAAAAGTTTCTT TTAAAGGAATTGAAAATTCTCAGGCACCAAAAATTGTCAGATGCTCTACCCATGGTACCCCAGGACCAGA AGGCAACCATATTTCAGATTTACCACTTCTAGACAGTCCCAATCCCTGGCTATCTTCTTCAGTGACTGCT CCATCCATGGTAGCCCCAGTCACTTTTGCATCTATTGTAGAAGAAGAACTACAACAAGAAGCAGCTCTTA TTAGAAGTCGAGAAAAACCGTTGGCTCTGATTCAGATTGAGGAGCATGCCATACAAGATTTATTGGTTTT CTATGAGGCATTTGGCAACCCTGAAGAGTTTGTCATTGTTGAAAGGACACCGCAGGGACCACTGGCAGTA CCTATGTGGAATAAGCATGGATGCTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001300906
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001300906.1, NP_001287835.1
RefSeq Size	6021 bp
RefSeq ORF	4017 bp
Locus ID	25998
UniProt ID	Q9P2D0
Protein Families	Druggable Genome
Gene Summary	Bruton tyrosine kinase (BTK) is a protein tyrosine kinase that is expressed in B cells, macrophages, and neutrophils. The protein encoded by this gene binds to BTK and downregulates BTK's kinase activity. In addition, the encoded protein disrupts BTK-mediated calcium mobilization and negatively regulates the activation of nuclear factor-kappa-B-driven transcription. This gene has a pseudogene on chromosome 18. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (2) uses an alternate in-frame splice acceptor site in the 3' coding region, compared to variant 1. It encodes a shorter isoform (2), compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.