DDX60L (NM_001291510) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001291510, the custom clone sequence may differ by one or more nucleotides ATGGGGTCAAAGGATCATGCAGTATTTTTCAGGGAAATGACACAGTTAATTTTGAATGAAATGCCAAAAG CTGGGTATTCCAGCATATTAAATGATTTTGTGGAATCTAATTTTTTTGTGATTGATGGAGATTCCTTGCT TGTCACATGCCTGGGTGTAAAATCATTCAAGTGGGGACAGAATCTCCACTTTTTCTATCTGGTTGAATGC TATCTTGTGGATCTTCTGAGTAACGGAGGACAATTCACCATAGTTTTCTTTAAGGATGCTGAATATGCAT ATTTTGATTTTCCTGAACTTCTTTCATTGAGGACCGCTTTAATTCTCCACCTTCAACACAATACTAACAT TGATGTGCAAACGGAGTTTTCTGGATGCTTATCACAAGATTGGAAGTTATTCTTGGAACAGCATTACCCG TATTTTCTGATAGTTTCAGAGGAAGGCCTGAGTGATTTACAAACGTACCTTTTTAACTTCCTAATCATAC ATTCCTGGGGAATGAAAGTCAATGTTGTGCTTTCATCAGGGCATGAATCTGATACTCTCAGATTTTATGC ATATACTATGGAAAGCACAGACAGAAACCAAACTTTTTCCAAGGAGAATGAAACAGTGATTCAGAGTGCA TATAAAAGCCTCATACAACACTTGGAAGAAATAAGGGTTTTAGTATTAGCAACTCATTTTGAACATTTGA AATGGAATGATATGATGGAAGAGGCGTATCAGACTCTATTTCTGCTTCAGCACCTATGGTCAGAAGGATC GGACATCCAGCGTGTTCTCTGTGTCACTTCATGTTCACTATCCTTGAGAATGTACCATCGTGTCTTAGTG CACAGTAATTGCCTATCCCTGCAGGAGGTGGAAGATTTCTGCAGACTGCGTTGCCTCTGTGTGGCTTTTC AACTCCACTTACCCCTTTCTCAGAGAGCTTGTTCTCGAGTCATCACATGCTCTTGGATTAGGAACAGTGA TTCTTTCTTAAAAATGAACAAGTGGTGTGAATATTTCATTTTAAGCAACTTAAACGTTTTTGGATGCTGG AATCTGAATTTAAATCATGTTTCTGACTTGTATGATGAGCAATTGTTAAAGAATATAGCCTTCTACTATG AATTTGAAAGTACTCAAGAACCACATTTGAATTTGGGAGATTCCATTAGGAGGGATTATGAAGACCTGTG GAATGTTGTGTCACACCTGGTTAAAGAATTTAACGTTGGAAAGTCTTTTCCTCTGAGAACAACAAGAAGA CATTTTCTTAGACAAGAGAAATCGGTCATTCAAGAAATCTCCTTGGAAAAGATGCCTAGTGTGGGCTTTA TTCCAATGACATCTGCTGTAATTGATGAGTTTGTTGGAGATATGATGAAGGATTTGCCTATTCTAAAGAG TGATGATCCGGTTGTTCCTTCACTGTTTAAACAAAAGACATCTGATGAACTTTTGCACTGGCATGCTCAA AGACTCCTTAGTGACGACTATGACAGGATCAAATGTCATGTTGATGAACAATCTAGAGATCCTCATGTTC TTGATTTCCTGAAAAAGATTCAGGATTATCAGCAATTTTATGGGAAATCGTTAGAATCAATCTCTACGAA AGTCATTGTGACTCAAACTACTCGGCCAAAGGAGGATTCCAGTGGTGCCAGTGGTGAAATATTACAGAAT ACCAAACCCCACCAAATTACCAAAAAGAGTAAGAAAAAGTCATTTCTCAAAGAAGATCAGAACAAAGCTC AGCAAAACGATGATCTGCTGTTTTCTATTGAAGAGGAGATGAAGAACAATTTACATTCTGGAATAAGGAA ATTGGAAGATTATTTGACATCATGTGCAAGTAATTCAGTGAAATTTGGAGTTGAAATGTTAGGATTAATT GCCTGCTTTAAAGCATGGAAAAAACATTGCCGAGGTGAAGGCAAAATTTCGAAAGATTTAAGTATAGCTG TTCAAATGATGAAAAGGATTCATTCACTCCTGGAGAGATACCCAGAAATTTTGGAAGCAGAACATCATCA ATATATAGCTAAATGCCTTAAATATTTAGGCTTTAATGATCTGGCAAACTCTTTGGATCCAACTCTGATA GGAGATGACAAAAATAAGAAGAAATATTCGATTGACATTGGACCAGCTCGGTTTCAACTGCAATACATGG GCCATTACTTGATAAGAGATGAAAGAAAAGATCGGGATCCCAGGGTCCAGGATTTTATTCCCAACGCATG GCAGCAGGAACTCCTGGATGTGGTAGATAAGAATGAGTCAGCAGTGATTGTTGCCCCAACGTCCTCAGGC AAAACCTATGCTTCCTACTACTGCATGGAGAAAGTGCTGAGGGAGAGCGATGTCGGGGTGGTTGTGTACG TTGCACCCGCAAAGTCCCTTGTTGGTCAAGTGGCTGCAACTGTTGAGAATCGTTTTACTAAAACGTTGCC TGCCGGCAGAACTCTATGCGGTGCTTTTACAAGAGATTATTGTCACAATGTACTAAACTGTCAGGTACTT ATTACAGTGCCGGAATGTTTTGAAATCCTGTTGCTTGCTCCTCATCGCCAAAAATGGGTGGAAAGGATCA GATATGTTATATTTGATGAGGTCCATTATCTTGGCAGAGAAGTTGGAGCAAAATTTTGGGAGCTCCTCCT TGTCATTATTCGATGTCCCTTTTTGGTTCTTTCAGCTACCATAAATAACCCAAATCTTCTCACCAAGTGG CTGCAATCAGTAAAACAGTACTGGAAACAGGCAGACAAGATTATGGAAGAGAAATGTATTTCTGAAAAAC AGGCTGACAAATGTCTCAACTTTCTCCAAGACCATTCATATAAAAATCAATCATATGAAGTTAGACTTGT GCTCTGTGGAGAGAGATACAATGATTTAGAGAAGCATATATGTTCAGTAAAACATGATGATGTTTATTTT GATCATTTTCATCCCTGTGCTGCGCTAACGACAGATATTATTGAAAAGTATGGATTCCCACCTGATCTTA CCCTCACCCCTCAAGAAAGCATCCAGCTTTATGATACCATGGCTCAAGTCTGGGAAACTTGGCCCAGGGC TCAGGAATTGTGTCCAGAGGAATTCATTCTTTTTAAGAATAAGATAGTCATTAAGAAGTTGGATGCTAGA AAATATGAAGAAAACTTAAAGGCAGAATTGACAAATTGGATTAAAAATGGCCAAGTGAAGAAGGTCAAAA GAGTACTGAAGAACCTTAGTCCGGATTCATTGTCTAGTTCAAAAGATATGGTGAAAATGTTTCCTCTTCT TGTTGAAAAGTTAAGACAAATGGATAAGTTGCCTGCAATATTTTTTTTGTTTAAGAATGATGATGTGGGA AAAAGAGCTGGAAGTGTGTGCACTTTTCTGGAGAAGACAGAGACAAAAAGCCATCCCCACACTGAATGTC ATAGTTATGTCTTTGCAATAGATGAAGTACTTGAAAAAGTGAGGAAGACACAGAAAAGGATTAGCACTAA AAAAAACCCAAAGAAGGCTGAAAAACTGGAAAGAAAAAAAGTGTATAGAGCTGAATATATTAATTTCCTG GAGAATCTGAAGATTCTGGAAATTTCTGAGGACTGCACGTATGCTGATGTCAAAGCCCTACACACTGAAA TTACCAGGAATAAAGACTCAACTTTGGAGAGGGTATTACCGCGAGTGCGATTTACAAGACACGGCAAAGA ACTGAAGGCTTTAGCACAAAGGGGGATTGGATATCATCACAGCAGCATGTATTTTAAAGAAAAAGAGTTT GTTGAGATACTCTTTGTAAAAGGGCTTATTAGGGTAGTGACAGCTACTGAAACACTTGCCTTAGGGATCC ACATGCCATGCAAATCTGTTGTTTTTGCCCAAGACTCAGTCTATCTGGATGCTTTAAATTACAGACAGAT CATGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001291510
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291510.1, NP_001278439.1
RefSeq Size	4611 bp
RefSeq ORF	3927 bp
Locus ID	91351
UniProt ID	Q5H9U9
Gene Summary	This gene encodes a member of the DExD/H-box helicase family of proteins, a subset of the super family 2 helicases. Members of the DExD/H-box helicase family share a conserved functional core comprised of two RecA-like globular domains. These domains contain conserved motifs that mediate ATP binding, ATP hydrolysis, nucleic acid binding, and RNA unwinding. In addition to functions in RNA metabolism, members of this family are involved in anti-viral immunity and act as cytosolic sensors of viral nucleic acids. The protein encoded by this gene has been shown to inhibit hepatitis C virus replication in response to interferon stimulation in cell culture. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]

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