SEC24B (NM_001300813) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	SEC24
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001300813, the custom clone sequence may differ by one or more nucleotides ATGTCGGCCCCCGCCGGGTCCTCTCACCCGGCCGCCAGCGCCCGGATCCCGCCCAAGTTCGGCGGAGCGG CCGTCTCAGGAGCCGCAGCGCCCGCGGGCCCGGGTGCGGGCCCGGCGCCGCACCAGCAGAACGAGACGGG ATTTCACCATGTTGCCCAAGCTAGTCTTGAACTCCTGGACCCAAGCAATCTGCCCGCCTCGGCCTCCCAA ATTGCTGGGAGTACAGGTCCAGCCCAGAATCAAATGCAGGTTCCATCTGGATATGGATTGCATCATCAAA ACTATATTGCTCCCTCAGGACATTACTCTCAAGGACCTGGGAAAATGACCTCATTGCCATTGGATACCCA GTGTGGTGATTACTACTCTGCTCTCTATACAGTACCAACACAAAATGTGACTCCTAACACAGTGAACCAG CAACCAGGAGCACAGCAGTTGTACAGCAGGGGTCCTCCTGCCCCTCATATTGTGGGATCCACTCTAGGAT CTTTCCAAGGTGCTGCATCGTCAGCATCCCATTTGCATACGAGTGCCTCCCAACCATACTCCTCTTTTGT GAATCACTACAATAGTCCAGCCATGTACTCTGCCAGCTCTTCTGTTGCGTCTCAGGGATTTCCCTCTACT TGTGGTCATTATGCTATGTCAACTGTTTCTAATGCCGCGTATCCTAGTGTTTCATATCCCTCTCTGCCTG CTGGTGATACATATGGGCAAATGTTTACCTCACAGAATGCTCCGACTGTTAGGCCAGTTAAAGATAATTC ATTCTCTGGTCAAAATACAGCTATCAGCCATCCATCGCCACTTCCACCTCTACCATCACAACAGCACCAC CAGCAGCAAAGTCTTTCAGGATACAGTACTCTAACGTGGTCATCTCCAGGCCTTCCATCGACTCAAGACA ATCTCATCCGAAACCACACAGGATCCCTGGCTGTAGCGAACAACAACCCAACCATTACTGTTGCAGATTC TTTATCCTGTCCTGTTATGCAAAATGTTCAGCCTCCCAAGTCCAGCCCAGTGGTATCCACTGTTTTATCA GGATCCTCAGGATCCTCATCAACAAGAACACCTCCCACTGCAAATCACCCAGTTGAGCCTGTGACCTCAG TTACACAGCCATCAGAGCTATTACAACAAAAAGGCGTGCAGTATGGTGAATATGTTAATAACCAAGCTAG CTCCGCACCAACTCCCTTGTCATCAACTTCCGATGATGAGGAAGAGGAGGAGGAGGATGAGGAAGCAGGT GTTGACAGCTCTTCTACCACAAGCAGTGCTTCTCCAATGCCCAACAGTTATGATGCCCTGGAAGGAGGCA GTTACCCAGATATGCTTTCTTCATCAGCAAGCAGTCCTGCTCCTGATCCCGCCCCTGAACCTGATCCTGC TTCTGCTCCAGCTCCAGCTTCAGCTCCAGCTCCTGTCGTCCCTCAGCCTTCAAAAATGGCTAAGCCTTTT GGCTATGGCTATCCAACACTTCAGCCTGGTTATCAGAATGCTACAGCACCACTTATTTCTGGAGTACAGC CCAGTAACCCGGTATATTCTGGATTCCAGCAGTATCCTCAATATCCTGGTGTGAACCAGCTATCCTCCAG TATAGGAGGATTGAGTCTTCAGAGTTCTCCACAACCAGAAAGCCTGAGACCTGTAAACCTTACTCAGGAG AGGAATATTTTACCTATGACTCCTGTTTGGGCTCCTGTACCTAACTTGAATGCAGACCTCAAAAAATTAA ACTGTAGCCCAGATTCATTTCGGTGTACTTTGACAAATATTCCACAGACACAGGCTTTACTGAATAAAGC TAAGCTTCCTTTAGGATTGTTGTTACATCCCTTCAGAGACCTAACGCAATTACCAGTGATAACATCAAAT ACCATTGTGAGGTGCCGATCCTGTCGAACGTATATTAACCCCTTTGTATCCTTCATTGATCAACGTAGAT GGAAATGCAATTTGTGCTATAGAGTAAACGATGTTCCTGAAGAATTTATGTATAACCCCCTTACCCGATC TTATGGAGAGCCTCATAAACGACCAGAAGTTCAGAATTCAACTGTGGAGTTCATTGCTTCTTCAGATTAC ATGCTGCGTCCTCCTCAACCTGCAGTTTACTTGTTTGTTTTAGATGTGTCTCATAATGCAGTGGAAGCTG GATATTTGACAATTTTGTGCCAGTCACTCCTAGAAAATCTAGACAAGCTTCCTGGAGATTCACGAACAAG AATAGGATTCATGACCTTTGATAGCACTATTCATTTCTACAATTTACAAGAAGGATTATCACAGCCTCAA ATGTTGATTGTGTCTGATATAGATGATGTTTTTCTACCTACACCGGATAGTTTACTTGTGAATCTATATG AAAGTAAAGAGCTTATAAAAGACTTACTGAATGCATTACCAAACATGTTCACCAATACAAGAGAAACACA CAGTGCCCTTGGTCCTGCACTTCAGGCTGCCTTTAAATTAATGTCTCCAACAGGTGGCCGTGTGTCTGTA TTTCAGACACAGTTACCTTCCTTGGGTGCAGGACTTCTGCAATCCAGAGAAGATCCTAATCAGAGATCAA GTACAAAGGTGGTACAACATCTTGGCCCTGCAACTGATTTTTATAAGAAACTTGCATTAGATTGCTCGGG ACAGCAAACTGCAGTGGATTTGTTCCTTTTAAGTTCACAGTATTCTGATCTTGCTTCTCTAGCTTGCATG TCCAAGTATTCTGCAGGGTGCATCTATTATTATCCATCATTCCACTATACTCACAATCCTTCACAAGCAG AAAAGTTACAAAAAGACCTAAAACGGTATCTCACAAGAAAAATTGGGTTTGAAGCTGTTATGAGAATAAG GTGTACTAAAGGTCTTTCAATGCACACTTTTCACGGTAACTTCTTTGTCCGTTCTACTGATTTGTTATCC CTTGCCAACATCAATCCTGATGCTGGATTTGCGGTGCAGTTGTCAATTGAAGAAAGTTTAACAGATACTT CCTTAGTATGTTTTCAAACAGCCCTATTATATACATCAAGCAAAGGTGAGCGGAGAATTAGAGTACATAC ACTTTGTTTGCCAGTGGTAAGTTCACTAGCAGATGTATATGCGGGAGTGGATGTACAAGCTGCCATCTGC CTTCTGGCAAACATGGCTGTGGATCGGTCCGTTTCATCAAGTCTGTCAGATGCAAGAGATGCCTTAGTGA ATGCTGTAGTGGACTCATTGTCTGCATATGGCTCAACTGTCTCAAATTTACAGCACTCTGCATTGATGGC GCCCAGCTCCCTCAAGTTGTTTCCTCTCTATGTTTTGGCCCTTCTCAAACAGAAAGCATTTAGAACGGGT ACAAGCACACGGCTGGATGATCGTGTATATGCCATGTGTCAGATAAAGTCTCAGCCACTTGTTCATCTAA TGAAAATGATTCATCCCAACTTATACAGGATAGACAGATTGACAGATGAGGGTGCAGTACATGTTAATGA CAGGATTGTACCACAGCCACCTCTTCAAAAATTGTCTGCAGAGAAGCTGACAAGAGAAGGTGCTTTCCTT ATGGACTGTGGCTCTGTTTTTTACATTTGGGTTGGGAAAGGCTGTGACAATAACTTCATAGAGGATGTGC TTGGATATACTAATTTTGCATCAATACCACAGAAAATGACACATCTTCCAGAGCTAGATACACTTTCATC AGAAAGAGCCAGATCCTTCATAACTTGGCTTAGAGACAGCAGACCATTAAGTCCAATCCTTCACATAGTA AAAGATGAGAGTCCTGCCAAAGCAGAATTTTTTCAGCATTTGATTGAAGACCGGACAGAGGCTGCATTTT CTTACTATGAATTTTTGCTTCATGTTCAGCAGCAGATTTGTAAGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001300813
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001300813.2, NP_001287742.1
RefSeq Size	4846 bp
RefSeq ORF	3897 bp
Locus ID	10427
UniProt ID	O95487
Gene Summary	The protein encoded by this gene is a member of the SEC24 subfamily of the SEC23/SEC24 family, which is involved in vesicle trafficking. The encoded protein is thought to be a cargo-binding component of the COPII vesicle, and is thought to be involved in the transport of secretory proteins from the endoplasmic reticulum to the Golgi apparatus. Mutations in this gene have been associated with neural tube defects, and are thought to be a result of a disruption in interactions with the protein encoded by the VANGL planar cell polarity protein 2 (VANGL2) gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015] Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (c).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.