MCAF1 (ATF7IP) (NM_181352) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AM; ATF-IP; MCAF; MCAF1; p621
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_181352, the custom clone sequence may differ by one or more nucleotides ATGCATCAGGACCAGAGATTCAGAATGGACAGTTTAGAAGAACCTCAGAAAAAAGTCTTTAAGGCTCGAA AAACGATGAGAGTGAGTGATCGTCAGCAACTTGAAGCAGTGTACAAGGTCAAAGAAGAACTGTTGAAAAC TGATGTCAAGCTGTTAAATGGCAACCATGAAAATGGAGATTTGGACCCAACCTCACCTTTGGAAAACATG GATTACATTAAAGACAAGGAAGAGGTGAATGGCATTGAAGAGATTTGTTTTGATCCTGAAGGAAGTAAAG CAGAATGGAAGGAAACACCCTGTATCCTAAGTGTTAATGTAAAAAACAAGCAGGATGATGATTTAAATTG TGAACCTTTGTCTCCCCATAATATAACTCCAGAACCAGTCTCTAAACTGCCTGCTGAACCAGTTTCTGGT GATCCAGCCCCTGGTGATCTGGATGCCGGAGATCCAGCCTCCGGAGTACTGGCCTCTGGTGATTCCACCT CTGGTGATCCCACCTCTAGCGAGCCCTCCTCTAGTGATGCTGCCTCTGGTGATGCAACCTCTGGTGATGC CCCTTCTGGTGATGTGTCCCCTGGTGATGCCACCTCTGGTGATGCCACTGCTGATGATCTCTCCTCTGGT GATCCCACCTCTAGTGATCCCATCCCAGGTGAACCGGTCCCTGTTGAACCCATTTCTGGTGATTGTGCCG CTGATGATATAGCCTCTAGTGAAATAACTTCTGTTGATCTGGCTTCTGGAGCACCAGCTTCCACTGATCC AGCCTCTGATGATCTGGCCTCTGGTGATCTATCCTCTAGTGAACTGGCCTCTGATGATCTGGCCACTGGT GAACTGGCCTCTGATGAGCTGACTTCTGAATCAACCTTTGATCGTACCTTTGAACCAAAGTCTGTACCAG TTTGTGAACCAGTTCCTGAAATTGACAATATAGAACCAAGTAGCAATAAAGATGATGATTTTCTTGAAAA AAATGGAGCTGATGAAAAATTAGAGCAAATTCAGAGTAAAGACTCATTGGATGAGAAAAATAAAGCTGAT AATAATATTGATGCTAATGAAGAAACTCTAGAAACAGATGATACAACTATTTGTTCAGATCGACCTCCTG AAAATGAAAAGAAGGTAGAGGAAGATATTATCACAGAGCTTGCTCTTGGAGAAGATGCTATATCTAGCAG TATGGAAATTGACCAAGGTGAAAAGAATGAAGATGAAACTTCTGCAGATCTTGTAGAAACGATTAATGAA AATGTTATTGAAGATAACAAAAGTGAGAATATCTTAGAAAATACAGACTCTATGGAGACAGATGAAATCA TTCCTATTTTGGAAAAGCTTGCACCTTCTGAGGATGAACTTACTTGCTTTTCTAAAACATCTCTCCTTCC AATCGATGAGACAAATCCAGATTTGGAAGAGAAAATGGAAAGTTCTTTTGGTTCACCATCTAAACAAGAA AGTAGTGAGAGTTTGCCAAAAGAAGCCTTTCTGGTCCTCTCTGATGAAGAGGATATTTCGGGTGAAAAAG ATGAGTCTGAAGTTATATCGCAAAATGAAACGTGCTCTCCAGCAGAAGTAGAAAGTAATGAAAAGGACAA CAAACCTGAGGAAGAAGAGCAAGTAATACATGAAGATGATGAAAGACCTTCTGAGAAAAATGAATTTTCT AGACGAAAACGTTCTAAATCAGAAGACATGGACAATGTACAGTCTAAACGTCGTCGATATATGGAAGAAG AATATGAGGCAGAATTTCAAGTAAAGATTACAGCCAAAGGAGACATTAACCAGAAACTTCAAAAGGTTAT ACAGTGGTTGCTGGAAGAAAAATTGTGTGCGCTGCAGTGTGCTGTATTTGATAAGACTTTGGCAGAATTG AAAACACGAGTGGAAAAGATTGAATGTAACAAGAGGCATAAAACAGTTCTCACTGAACTACAGGCCAAGA TAGCCAGGTTAACCAAACGCTTTGAAGCAGCCAAAGAAGATCTTAAGAAAAGACATGAACATCCACCCAA CCCACCAGTATCACCAGGAAAAACTGTAAATGATGTCAACAGCAATAATAACATGTCTTACAGAAATGCA GGCACAGTGAGACAGATGCTGGAGTCCAAAAGAAATGTAAGCGAGAGTGCACCACCATCCTTTCAAACTC CTGTGAATACAGTATCTTCAACCAATCTTGTCACTCCTCCAGCAGTTGTCAGTAGTCAACCTAAATTGCA GACTCCAGTGACTTCGGGTTCCCTCACAGCAACGTCAGTTCTTCCTGCACCCAATACAGCTACTGTAGTT GCTACTACTCAGGTGCCTAGTGGAAATCCCCAGCCTACAATCTCTTTACAGCCTTTGCCAGTGATTTTGC ATGTACCTGTTGCAGTATCCTCCCAGCCTCAGCTTCTACAGAGCCATCCAGGGACTTTGGTGACTAATCA ACCATCTGGCAATGTTGAATTCATTTCTGTGCAAAGCCCACCTACAGTGAGTGGTCTTACCAAAAATCCA GTATCCTTGCCATCCTTGCCAAATCCCACTAAACCAAACAACGTTCCTTCTGTGCCCAGTCCTAGTATTC AAAGGAACCCTACTGCCAGTGCTGCACCATTGGGAACAACACTTGCTGTGCAGGCTGTTCCAACAGCACA CTCTATTGTACAAGCCACAAGGACTTCTTTACCCACAGTGGGCCCATCAGGACTCTATAGTCCATCAACT AATCGAGGTCCTATACAGATGAAAATTCCAATTTCTGCATTTAGTACTTCGTCTGCTGCAGAACAGAACA GCAATACCACCCCAAGAATTGAAAACCAGACAAACAAAACAATAGATGCTTCTGTCAGTAAGAAAGCAGC TGATAGCACATCACAGTGTGGAAAAGCCACTGGCAGTGATTCAAGTGGTGTCATTGATCTCACAATGGAT GATGAAGAGAGTGGAGCTTCACAAGACCCCAAAAAACTAAATCACACTCCTGTATCAACCATGAGTTCTT CTCAGCCTGTGTCACGACCATTGCAACCCATACAACCAGCACCGCCTCTTCAACCATCTGGGGTGCCAAC AAGTGGACCATCTCAGACCACCATACACTTACTACCTACAGCTCCAACTACCGTGAATGTAACACATCGT CCAGTAACTCAGGTGACCACAAGACTCCCTGTACCAAGAGCTCCTGCAAACCACCAGGTGGTTTATACAA CTCTTCCTGCACCACCAGCTCAGGCTCCCTTGCGAGGAACTGTTATGCAGGCTCCTGCTGTTCGGCAGGT CAATCCCCAAAATAGTGTTACAGTTCGAGTGCCTCAAACAACCACATATGTTGTAAACAATGGACTAACC CTGGGATCAACAGGACCTCAGCTCACAGTGCATCACCGACCACCACAAGTGCATACTGAGCCCCCACGCC CCGTGCACCCAGCACCCTTACCAGAAGCTCCACAACCACAGCGTCTGCCCCCAGAAGCTGCCAGCACATC TCTGCCTCAGAAGCCACACTTGAAGTTAGCACGCGTTCAGAGTCAAAATGGCATAGTACTGTCATGGAGT GTCCTGGAGGTGGATCGAAGCTGTGCCACTGTTGATAGCTACCATCTCTATGCTTACCATGAGGAACCCA GTGCCACTGTGCCCTCACAATGGAAAAAGATTGGGGAAGTCAAGGCACTTCCCTTGCCCATGGCATGTAC TCTCACCCAGTTTGTATCTGGTAGCAAATACTACTTTGCAGTACGAGCCAAGGATATTTATGGACGTTTT GGGCCTTTCTGTGATCCTCAGTCAACAGATGTGATCTCTTCTACCCAGAGCAGTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_181352
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_181352.1, NP_851997.1
RefSeq Size	9019 bp
RefSeq ORF	3837 bp
Locus ID	55729
UniProt ID	Q6VMQ6
Protein Families	Transcription Factors
Gene Summary	ATF7IP is a multifunctional nuclear protein that associates with heterochromatin. It can act as a transcriptional coactivator or corepressor depending upon its binding partners (summary by Liu et al., 2009 [PubMed 19106100]).[supplied by OMIM, Nov 2010] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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