RBM16 (SCAF8) (NM_001286199) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	RBM16
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001286199, the custom clone sequence may differ by one or more nucleotides ATGGAGGCCGTGAAGACCTTCAATAGCGAGTTGTATTCCCTGAATGACTATAAACCACCCATTTCGAAAG CGAAAATGACCCAAATTACTAAGGCAGCCATCAAAGCTATTAAGTTCTATAAACATGTGGTACAGAGTGT TGAGAAGTTTATTCAGAAATGTAAACCAGAATACAAAGTACCTGGACTTTATGTTATTGACTCCATTGTG CGACAATCCCGACATCAGTTTGGTCAAGAAAAGGATGTGTTTGCACCCAGATTTAGTAATAACATCATTA GCACTTTCCAGAATTTATATCGTTGCCCTGGGGATGACAAGAGTAAAATAGTGAGAGTACTAAACTTATG GCAGAAGAATAATGTATTTAAGAGTGAGATTATTCAGCCCCTTTTGGATATGGCAGCCGGGATTCCGCCT CCAGTTGTCACACCTGTTTTGGCCAGCACTACCACTGCTATGAGCAATACTCCAGGAACTCCTGTGACAC CTGTTACTCCGGCCAATGTGGTCCAAGGCTTACCTGATCCGTGGGTATCTCAGATAACAAATACAGATAC ACTTGCGGCTGTAGCTCAGATCTTGCAAAGTCCTCAAGGCCAGCAGCTTCAACAATTAATACAAACCTTA CAGATACAACAACAGAAGCCCCAGCCTTCCATTCTGCAGGCCCTAGATGCTGGTCTTGTTGTTCAGTTGC AAGCTCTTACGGCACAACTTACAGCTGCAGCTGCAGCTGCCAACACTCTTACTCCCTTAGAACAGGGAGT CTCCTTTAACAAGAAGTTGATGGATAGGTTTGATTTTGGGGAAGACTCTGAGCATAGTGAAGAACCCAAA AAGGAAATTCCAGCTTCACAACTTTCTCACGTTTCAGAATCTGTGAACAATTCCATTTTTCATCAGATAG CAGAACAACTACAACAGCAAAACCTAGAACATCTCAGACAGCAGCTCTTGGAGCAGCAACAGCCTCAAAA GGCCACTCCTCAGGATAGTCAGGAAGGAACCTTTGGGTCAGAGCATTCAGCGTCACCATCACAAGGGAGT AGTCAGCAGCATTTTCTTGAACCTGAAGTCAATTTGGATGATTCCATAGATATTCAGCAACAGGATATGG ATATAGATGAAGGGCAAGATGGAGTGGAAGAGGAGGTCTTTGAACAAGAAGCTAAGAAAGTGGCGGTTCG CTCAAGATCAAGAACACATTCACGATCTCGTTCAAGATCACCAAGAAAACGAAGGTCTAGGTCACGGTCT GGCTCTAGAAAGCGTAAACACAGAAAGCGATCACGCTCCCGCTCAAGAGAAAGAAAGAGGAAATCATCAC GGTCGTATTCAAGTGAAAGGAGAGCCAGAGAAAGGGAGAAAGAACGACAGAAAAAGGGATTACCTCCAAT TAGATCTAAAACACTAAGTGTATGTAGTACTACTCTCTGGGTTGGGCAAGTGGACAAGAAGGCAACACAG CAAGACTTAACCAACCTGTTTGAAGAGTTTGGACAGATTGAATCCATTAATATGATTCCTCCCCGGGGCT GTGCTTATGTCTGCATGGTTCATCGACAAGATGCATTTCGAGCTCTTCAGAAACTCAGTTCTGGATCATA TAAAATTGGGTCCAAGGTCATTAAGATCGCTTGGGCTTTAAACAAAGGTGTAAAAACAGAATACAAACAA TTCTGGGATGTGGATCTTGGAGTTACATATATACCATGGGAAAAAGTTAAAGTGGATGACTTGGAAGGTT TTGCAGAAGGAGGCATGATTGATCAGGAGACTGTAAATACTGAGTGGGAAACTGTGAAAAGCTCAGAACC TGTTAAAGAGACGGTCCAGACAACTCAGAGCCCAACTCCAGTTGAAAAGGAGACAGTGGTCACAACCCAG GCAGAGGTTTTCCCTCCTCCTGTTGCTATGTTGCAGATTCCAGTGGCGCCAGCCGTGCCTACAGTTAGTT TAGTCCCACCAGCATTTCCTGTGTCGATGCCGGTTCCTCCTCCTGGATTCAGTCCAATCCCTCCACCTCC TTTTTTAAGAGCAAGTTTTAACCCTTCACAACCACCACCTGGTTTCATGCCGCCTCCAGTTCCCCCACCT GTTGTGCCACCCCCTACGATTCCACCAGTAGTACCAACATCTTTAGTGCAGCCGTCATTATCCATGACAC CGGAAACTGTGAAAGATGTTGGATTTGGTAGCCTTGTTATACCAGGCGGTTCTGTTGCCAGCAATCTTGC TACTTCCGCTCTGCCAGCTGGAAATGTTTTTAATGCTCCAACTAAACAGGCAGAGCCTGAAGAAAAAGTA CCTCATCTTATAGACCACCAGATTTCTTCTGGTGAAAACACCAGATCAGTGATTCCAAATGATATTTCAA GTAATGCTGCAATTTTAGGAGGACAGCCGCCAAATGTGACAAGCAATTCTGGAATTCTGGGAGTCCAAAG ACCAAATGTATCAAGTAATTCTGAAATTCTTGGGGTCCGGCCATCTAATGTTTCCAGTAGTTCTGGGATT ATTGCAGCCCAACCACCAAATATTCTAAATAACTCTGGAATATTGGGAATACAGCCACCCAGTGTGTCAA ATAGTTCTGGACTTTTGGGAGTGCTACCCCCAAATATACCTAACAATTCTGGACTTGTAGGAGTACAGCC ACCAAATGTTCCAAATACTCCTGGACTTCTGGGAACACAGCCACCAGCTGGACCTCAAAACTTACCCCCT TTAAGTATCCCTAATCAAAGGATGCCCACAATGCCAATGTTAGACATTCGTCCGGGACTAATACCACAGG CACCTGGGCCAAGATTCCCTTTAATACAGCCTGGAATTCCACCCCAACGGGGAATCCCACCCCCATCGGT ACTTGATTCAGCTCTTCATCCACCACCCCGTGGACCTTTTCCTCCAGGAGATATTTTTAGTCAACCAGAA AGACCTTTTTTAGCTCCTGGAAGACAAAGCGTAGACAATGTTACTAACCCAGAAAAAAGGATACCACTTG GGAATGATAACATTCAACAGGAAGGAGATAGAGATTACCGGTTTCCTCCTATAGAAACCAGGGAAAGCAT TAGTAGACCTCCCCCTGTGGATGTTAGAGATGTGGTTGGGCGGCCTATAGATCCAAGAGAAGGTCCTGGA CGGCCTCCACTAGATGGTAGGGATCATTTTGGAAGACCTCCTGTAGATATAAGAGAGAATCTTGTGAGGC CAGGTATAGATCATCTTGGTCGAAGAGACCACTTTGGCTTTAATCCAGAGAAGCCCTGGGGGCATAGAGG AGATTTTGATGAGAGAGAGCATCGGGTTCTACCGGTCTATGGTGGTCCAAAAGGCTTACATGAAGAAAGA GGTAGATTTCGGTCTGGAAACTATCGATTTGATCCTAGAAGTGGTCCTTGGAACCGAGGATTTGGACAAG AAGTTCACAGAGATTTTGATGACCGCAGAAGACCCTGGGAGAGGCAAAGGGATAGGGATGACAGAGATTT TGATTTCTGCAGAGAAATGAATGGAAATCGTCTTGGACGAGACAGAATTCAAAACACTTGGGTTCCCCCT CCTCATGCTCGGGTTTTTGATTATTTTGAAGGGGCCACTTCTCAACGAAAAGGTGATAATGTGCCTCAGG TTAATGGTGAAAATACAGAGAGACATGCTCAGCCACCACCTATACCAGTACAGAATGATCCTGAACTTTA TGAAAAACTGACATCTTCAAATGAAATAAACAAGGAGAAGAGTGACACAGTTGCTGATATAGAAAGTGAA CCAGTGGTAGAAAGCACAGAAACTGAGGGGACATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286199
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286199.1, NP_001273128.1
RefSeq Size	4897 bp
RefSeq ORF	3816 bp
Locus ID	22828
UniProt ID	Q9UPN6
Gene Summary	Anti-terminator protein required to prevent early mRNA termination during transcription (PubMed:31104839). Together with SCAF4, acts by suppressing the use of early, alternative poly(A) sites, thereby preventing the accumulation of non-functional truncated proteins (PubMed:31104839). Mechanistically, associates with the phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit (POLR2A), and subsequently binds nascent RNA upstream of early polyadenylation sites to prevent premature mRNA transcript cleavage and polyadenylation (PubMed:31104839). Independently of SCAF4, also acts as a positive regulator of transcript elongation (PubMed:31104839).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) contains an alternate 5' exon and it thus differs in its 5' UTR and initiates translation at an downstream in-frame start codon, compared to variant 1. The encoded isoform (d) is shorter at the N-terminus, compared to isoform a. Both variants 4 and 5 encode isoform d.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.