GAK (NM_001286833) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DNAJ26; DNAJC26
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001286833, the custom clone sequence may differ by one or more nucleotides ATGTCGCTGCTGCAGTCGGCGCTCGACTTCTTGGCGGGTCCAGGCTCCCTGGGCGGTGCTTCCGGCCGCG ACCAGAGTGACTTCGTGGGGCAGACGGTGGAACTGGGCGAGCTGCGGCTGCGGGTGCGGCGGGTCCTGGC CGAAGGGCAGCTGGTGGAATTTTTGAAGAAAATGGAATCTCGAGGCCCCCTTTCGTGCGACACGGTTCTG AAGATCTTCTACCAGACGTGCCGCGCCGTGCAGCACATGCACCGGCAGAAGCCGCCCATCATCCACAGGG ACCTCAAGGTTGAGAACTTGTTGCTTAGTAACCAAGGGACCATTAAGCTGTGTGACTTTGGCAGTGCCAC GACCATCTCGCACTACCCTGACTACAGCTGGAGCGCCCAGAGGCGAGCCCTGGTGGAGGAAGAGATCACG AGGAATACAACACCAATGTATAGAACACCAGAAATCATAGACTTGTATTCCAACTTCCCGATCGGCGAGA AGCAGGATATCTGGGCCCTGGGCTGCATCTTGTACCTGCTGTGCTTCCGGCAGCACCCTTTTGAGGATGG AGCGAAACTTCGAATAGTCAATGGCGCCATGCTGCAGGTGAACCCGGAGGAGCGGCTGTCCATCGCCGAG GTGGTGCACCAGCTGCAGGAGATCGCGGCCGCCCGCAACGTGAACCCCAAGTCTCCCATCACAGAGCTCC TGGAGCAGAATGGAGGCTACGGGAGCGCCACACTGTCCCGAGGGCCACCCCCTCCCGTGGGCCCCGCTGG CAGTGGCTACAGTGGAGGCCTGGCGCTGGCGGAGTACGACCAGCCGTATGGCGGCTTCCTGGACATTCTG CGGGGTGGGACAGAGCGGCTCTTCACCAACCTCAAGGACACCTCCTCCAAGGTCATCCAGTCCGTCGCTA ATTATGCAAAGGGTGACCTGGACATATCTTACATCACATCCAGAATTGCAGTGATGTCATTCCCAGCAGA AGGTGTGGAGTCAGCGCTCAAAAACAACATCGAAGATGTGCGGTTGTTCCTGGACTCCAAGCACCCAGGG CACTATGCCGTCTACAACCTGTCCCCGAGGACCTACCGGCCCTCCAGGTTCCACAACCGGGTCTCCGAGT GTGGCTGGGCAGCACGGCGGGCCCCACACCTGCACACCCTGTACAACATCTGCAGGAACATGCACGCCTG GCTGCGGCAGGACCACAAGAACGTCTGCGTCGTGCACTGCATGGACGGGAGAGCCGCGTCTGCTGTGGCC GTCTGCTCCTTCCTGTGCTTCTGCCGTCTCTTCAGCACCGCGGAGGCCGCCGTGTACATGTTCAGCATGA AGCGCTGCCCACCAGGCATCTGGCCATCCCACAAAAGGTACATCGAGTACATGTGTGACATGGTGGCGGA GGAGCCCATCACACCCCACAGCAAGCCCATCCTGGTGAGGGCCGTGGTCATGACACCCGTGCCGCTGTTC AGCAAGCAGAGGAGCGGCTGCAGGCCCTTCTGCGAGGTCTACGTGGGGGACGAGCGTGTGGCCAGCACCT CCCAGGAGTACGACAAGATGCGGGACTTTAAGATTGAAGATGGCAAAGCGGTGATTCCCCTGGGCGTCAC GGTGCAAGGAGACGTGCTCATCGTCATCTATCACGCCCGGTCCACTCTGGGCGGCCGGCTGCAGGCCAAG ATGGCATCCATGAAGATGTTCCAGATTCAGTTCCACACGGGGTTTGTGCCTCGGAACGCCACCACTGTGA AATTTGCCAAGTATGACCTGGACGCGTGTGACATTCAAGAAAAATACCCGGATTTATTTCAAGTGAACCT GGAAGTGGAGGTGGAGCCCAGGGACAGGCCGAGCCGGGAAGCCCCACCATGGGAGAACTCGAGCATGAGG GGGCTGAACCCCAAAATCCTGTTTTCCAGCCGGGAGGAGCAGCAAGACATTCTGTCTAAGTTTGGGAAGC CGGAGCTTCCCCGGCAGCCTGGCTCCACGGCTCAGTATGATGCTGGGGCAGGGTCCCCGGAAGCCGAACC CACAGACTCTGACTCACCGCCAAGCAGCAGCGCGGACGCCAGTCGCTTCCTGCACACGCTGGACTGGCAG GAAGAGAAGGAGGCAGAGACTGGTGCAGAAAATGCCTCTTCCAAGGAGAGCGAGTCTGCCCTGATGGAGG ACAGAGACGAGAGTGAGGTGTCAGATGAAGGGGGATCCCCGATCTCCAGCGAGGGCCAGGAACCCAGGGC CGACCCAGAGCCCCCCGGCCTGGCAGCAGGGCTGGTGCAGCAGGACTTGGTTTTTGAGGTGGAGACACCG GCTGTGCTGCCAGAGCCTGTGCCACAGGAAGACGGGGTCGACCTCCTGGGCCTGCACTCCGAGGTGGGCG CAGGGCCAGCTGTACCCCCGCAGGCCTGCAAGGCCCCCTCCAGCAACACCGACCTGCTCAGCTGCCTCCT TGGGCCCCCTGAGGCCGCCTCCCAGGGGCCCCCGGAGGATCTGCTCAGCGAGGACCCGCTGCTCCTGGCA AGCCCGGCCCCTCCCCTGAGCGTGCAGAGCACCCCAAGAGGAGGGCCCCCTGCCGCTGCTGACCCCTTTG GCCCGCTTCTGCCGTCTTCAGGCAACAACTCCCAGCCCTGCTCCAATCCTGATCTCTTCGGCGAATTTCT CAATTCGGACTCTGTGACCGTCCCACCATCCTTCCCGTCTGCCCACAGTGCTCCGCCCCCATCCTGCAGC GCCGACTTCCTGCACCTGGGGGATCTGCCAGGAGAGCCCAGCAAGATGACAGCCTCGTCCAGCAACCCAG ACCTGCTGGGAGGATGGGCTGCCTGGACCGAGACTGCAGCATCGGCAGTGGCCCCCACGCCAGCCACAGA AGGCCCCCTCTTCTCTCCTGGAGGTCAGCCGGCCCCTTGTGGCTCTCAGGCCAGCTGGACCAAGTCTCAG AACCCGGACCCATTTGCTGACCTTGGCGACCTCAGCTCCGGCCTCCAAGGCTCACCAGCTGGATTCCCTC CTGGGGGCTTCATTCCCAAAACGGCCACCACGCCCAAAGGCAGCAGCTCCTGGCAGACAAGTCGGCCGCC AGCCCAGGGCGCCTCATGGCCCCCTCAGGCCAAGCCGCCCCCCAAAGCCTGCACACAGCCAAGGCCTAAC TATGCCTCGAACTTCAGTGTGATCGGGGCGCGGGAGGAGCGGGGGGTCCGCGCACCCAGCTTTGCTCAAA AGCCAAAAGTCTCTGAGAACGACTTTGAAGATCTGTTGTCCAATCAAGGCTTCTCCTCCAGGTCTGACAA GAAAGGGCCAAAGACCATTGCAGAGATGAGGAAGCAGGACCTGGCTAAAGACACGGACCCACTCAAGCTG AAGCTCCTGGACTGGATTGAGGGCAAGGAGCGGAACATCCGGGCCCTGCTGTCCACGCTGCACACAGTGC TGTGGGACGGGGAGAGCCGCTGGACGCCCGTGGGCATGGCCGACCTGGTGGCTCCGGAGCAAGTGAAGAA GCACTATCGCCGCGCGGTGCTGGCTGTGCACCCCGACAAGGCTGCGGGGCAGCCGTACGAGCAGCACGCC AAGATGATCTTCATGGAGCTGAATGACGCCTGGTCGGAGTTTGAGAACCAGGGCTCCCGGCCCCTCTTCT GA
Restriction Sites	SgfI-RsrII
ACCN	NM_001286833
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286833.1, NP_001273762.1
RefSeq Size	4240 bp
RefSeq ORF	3642 bp
Locus ID	2580
Protein Families	Druggable Genome, Protein Kinase
Gene Summary	In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I. Cyclin G is a direct transcriptional target of the p53 tumor suppressor gene product and thus functions downstream of p53. GAK is an association partner of cyclin G and CDK5. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015] Transcript Variant: This variant (2) lacks an alternate in-frame segment in the 5' end compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.

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