PARD3B (NM_001302769) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ALS2CR19; PAR3B; PAR3beta; PAR3L
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001302769, the custom clone sequence may differ by one or more nucleotides ATGAAAGTGACCGTGTGCTTCGGCAGGACGGGCATCGTGGTGCCCTGCAAGGAGGGCCAGCTGCGCGTCG GCGAGCTCACCCAGCAGGCGCTGCAGCGGTACCTGAAGACCCGGGAGAAGGGTCCTGGTTACTGGGTGAA GATTCATCACTTAGAATATACAGATGGAGGAATCCTGGATCCAGATGATGTCTTGGCAGATGTTGTTGAA GATAAAGACAAGCTGATTGCTGTGTTTGAAGAACAAGAACCACTCCACAAGATTGAGAGCCCCAGTGGAA ACCCTGCAGATCGGCAGAGCCCAGATGCTTTTGAGACAGAAGTGGCCGCCCAACTGGCCGCATTTAAGCC AATTGGTGGGGAGATTGAAGTAACCCCTTCTGCTCTAAAACTAGGCACTCCACTGCTGGTGAGGAGAAGC AGTGACCCAGTGCCAGGCCCACCTGCTGATACCCAGCCAAGCGCTTCACACCCTGGTGGCCAGAGTCTGA AACTGGTTGTTCCAGATTCCACGCAGAACTTGGAAGACAGAGAAGTTTTGAATGGTGTACAGACAGAACT ACTAACTTCGCCAAGAACTAAGGACACATTGAGTGATATGACAAGAACAGTGGAGATTTCTGGGGAAGGA GGCCCATTGGGAATACATGTAGTGCCCTTCTTTTCATCTCTGAGTGGAAGGATTCTAGGACTCTTCATCC GAGGCATTGAAGACAACAGCAGGTCCAAGCGGGAGGGACTATTTCACGAAAATGAATGTATTGTAAAAAT CAACAATGTGGATCTCGTAGACAAAACCTTTGCTCAGGCTCAAGATGTCTTCCGCCAGGCAATGAAATCT CCAAGTGTGCTCCTCCACGTGCTTCCTCCACAAAACCGTGAACAGTATGAAAAGTCAGTCATTGGCTCTC TTAACATTTTTGGTAATAATGATGGCGTTTTGAAAACCAAAGTGCCGCCTCCTGTCCATGGAAAATCGGG ACTAAAGACAGCAAATCTCACAGGAACCGATAGTCCTGAAACAGATGCATCAGCTTCCCTGCAACAAAAC AAGAGTCCCCGAGTACCAAGGCTGGGAGGAAAACCATCCTCTCCCTCACTCTCGCCTCTCATGGGATTTG GCAGCAATAAAAATGCAAAGAAAATTAAGATTGACCTAAAGAAAGGCCCTGAAGGACTTGGTTTCACTGT GGTTACCAGAGACTCTTCCATACATGGTCCCGGTCCCATTTTTGTAAAAAACATTTTACCAAAGGGAGCA GCAATAAAAGATGGCCGCCTACAATCAGGGGACAGAATTTTGGAGGTAAATGGGAGAGATGTCACCGGAC GAACCCAGGAAGAGCTTGTGGCCATGCTCAGGAGCACCAAGCAGGGGGAGACAGCATCGCTGGTCATTGC CCGCCAAGAAGGACATTTTCTGCCCCGAGAGTTGAAAGGAGAACCTGACTGCTGTGCACTCTCTCTGGAG ACAAGCGAGCAGCTCACCTTTGAGATCCCCCTGAATGATTCAGGTTCTGCTGGCCTCGGGGTGAGCTTAA AAGGGAACAAATCCAGAGAAACTGGAACAGACTTGGGGATTTTTATCAAATCCATCATTCATGGAGGCGC TGCTTTTAAGGATGGTCGTCTGCGAATGAATGACCAGCTGATTGCAGTTAATGGGGAATCTCTTTTGGGA AAGTCCAACCACGAAGCTATGGAAACACTTAGGCGGTCAATGTCCATGGAGGGAAACATCCGAGGGATGA TCCAGTTGGTGATTCTGAGGAGGCCAGAGAGACCAATGGAGGATCCTGCAGAGTGTGGGGCATTTTCCAA GCCATGCTTTGAGAACTGTCAAAATGCTGTAACCACCTCTAGGCGAAATGATAATAGTATCCTGCATCCA CTTGGCACTTGCAGTCCACAAGACAAACAGAAAGGTCTATTGCTGCCCAATGACGGATGGGCCGAGAGTG AAGTTCCACCTTCTCCAACACCACATTCTGCTCTGGGATTGGGCCTCGAAGATTACAGCCACAGCTCTGG GGTGGATTCAGCAGTATATTTTCCAGATCAGCACATCAACTTCAGATCTGTGACACCGGCCAGGCAGCCT GAATCAATTAATTTGAAAGCCTCGAAGAGCATGGACCTTGTGCCAGATGAAAGCAAGGTTCACTCATTGG CTGGACAAAAATCGGAATCTCCAAGCAAAGATTTTGGTCCAACTCTGGGTTTGAAAAAGTCCAGCTCCTT GGAGAGTCTGCAGACTGCAGTGGCCGAGGTCAGGAAGAATGACCTTCCCTTTCACAGGCCCCGGCCGCAC ATGGTTCGAGGCCGAGGCTGCAATGAGAGCTTTAGAGCAGCCATTGACAAATCCTACGATGGACCTGAAG AAATAGAAGCTGACGGTCTGTCTGATAAGAGCTCTCACTCTGGCCAAGGAGCTCTGAATTGTGAGTCTGC CCCTCAGGGGAATTCGGAGCTAGAGGACATGGAAAATAAAGCCAGGAAAGTCAAAAAAACGAAAGAGAAG GAGAAGAAAAAGGAAAAGGGCAAATTGAAAGTCAAGGAGAAAAAGCGCAAAGAGGAGAATGAAGATCCAG AAAGGAAAATAAAGAAGAAGGGCTTCGGCGCCATGCTGAGATTTGGAAAGAAGAAAGAGGATAAGGGTGG AAAGGCTGAGCAGAAAGGTACTCTGAAACATGGTGGCCTGAGAGAAGAAGAGCTGGAGAAAATGAAAGAA GAGCGTGAAAGGATTGGAGCAAAACATCAGGAGCTAAGGGAAAAGCAGGCAAGAGGTCTTCTTGATTATG CTACTGGTGCAATTGGATCAGTGTATGATATGGATGATGATGAAATGGACCCCAATTATGCCAGAGTGAA CCACTTTCGGGAACCATGCACATCAGCAAATGTCTTTAGATCTCCATCTCCCCCTCGAGCTGGACCATTT GGTTACCCTCGGGATGGCCATCCACTGTCTCCAGAAAGAGACCACTTAGAGGGTCTCTATGCCAAGGTCA ACAAGCCATACCATCCACTGGTTCCAGCTGACAGTGGCCGTCCTACGGGTGGAAGCACTGACCGTATCCA GAAGTTGCGGAAAGAGTATTATCAGGCTCGGAGGGAAGGTTTCCCTTTATATGAAGACGACGAAGGAAGA GCAAGGCCATCTGAGTATGACCTACTCTGGGTGCCTGGAAGGGGTCCAGATGGGAATGCACACAACCTCC GCTTTGAAGGGATGGAGAGGCAGTACGCATCCTTACCCAGGGGAGGACCCGCAGATCCTGTAGACTATCT GCCAGCAGCACCTCGGGGGCTCTACAAGGAAAGGGAGCTTCCCTATTATCCAGGGGCTCATCCTATGCAC CCTCCCAAAGGGAGCTATCCCCGCCCCACAGAGCTCAGGGTGGCAGATCTCCGGTATCCTCAGCACTACC CACCCCCGCCAGCTCCCCAGCACAAAGGACCCTTTCGACAAGACGTTCCGCCTTCCCCTCCCCAGCACCA AAGAATGCCAGCCTATCAGGAAACAGGCAGACCAGGGCCCCGTGGGGGCAGCCCAGACCAGTACCCTTAC CGAACCCAGGATTCCCGGCAGAAGAACCCCATGACTGCAGCCGTATAG
Restriction Sites	SgfI-MluI
ACCN	NM_001302769
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302769.1, NP_001289698.1
RefSeq Size	8174 bp
RefSeq ORF	3618 bp
Locus ID	117583
UniProt ID	Q8TEW8
Gene Summary	Putative adapter protein involved in asymmetrical cell division and cell polarization processes. May play a role in the formation of epithelial tight junctions.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (Par3La). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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