PRDM15 (NM_001282934) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C21orf83; PFM15; ZNF298
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001282934, the custom clone sequence may differ by one or more nucleotides ATGCCCCGCCGCCGCCCCCCGGCTTCCGGTGCTGCGCAGTTTCCGGAGCGGATCGCAACCCGGAGTCCGG ATCCGATCCCGCTCTGCACATTCCAAAGGCAGGTGTCAGAGATGGCTGAAGATGGGAGCGAAGAGATCAT GTTCATCTGGTGTGAAGACTGCAGCCAGTACCACGACTCCGAATGTCCCGAGCTGGGCCCAGTGGTCATG GTCAAAGACTCCTTTGTGTTAAGCAGGGCAAGGTCATCCCTTCCTCCCAACTTGGAGATCAGACGACTGG AAGATGGAGCCGAGGGGGTGTTCGCCATCACTCAGCTCGTCAAGCGGACACAGTTCGGTCCCTTTGAGTC CAGGAGGGTCGCCAAATGGGAAAAGGAGTCTGCATTTCCCCTGAAGGTGTTCCAGAAGGACGGGCACCCC GTGTGCTTCGACACCTCCAACGAGGATGACTGCAACTGGATGATGCTGGTGCGGCCAGCGGCGGAGGCCG AGCACCAGAACCTGACGGCCTACCAGCACGGCAGCGACGTGTACTTCACCACCTCCAGAGACATCCCCCC GGGTACCGAGCTGCGCGTGTGGTATGCGGCCTTCTATGCCAAGAAGATGGACAAGCCCATGCTGAAGCAG GCCGGCTCTGGCGTCCACGCTGCAGGCACCCCAGAAAACAGCGCCCCCGTGGAGTCGGAGCCCAGCCAGT GGGCGTGTAAAGTGTGTTCTGCCACCTTCCTGGAGCTGCAGCTCCTCAATGAACATCTGTTGGGCCACTT AGAACAAGCCAAAAGCCTTCCTCCAGGCAGCCAAAGCGAGGCAGCAGCTCCCGAGAAGGAGCAGGACACA CCCCGGGGGGAACCCCCTGCAGTGCCCGAGAGCGAGAATGTTGCCACCAAAGAACAGAAGAAAAAGCCTC GAAGGGGGAGAAAACCCAAAGTGTCCAAAGCTGAGCAGCCTCTAGTCATCGTGGAAGACAAGGAACCCAC AGAGCAAGTGGCAGAGATCATTACCGAGGTCCCTCCGGATGAGCCTGTGAGTGCAACGCCAGATGAGCGG ATCATGGAGCTGGTTCTGGGGAAGCTGGCCACCACCACCACTGACACCAGCTCGGTTCCAAAGTTCACCC ATCATCAGAATAACACCATCACGCTCAAGAGGAGCTTAATTCTCTCAAGCAGACACGGCATCCGGCGCAA GCTCATCAAACAGCTCGGGGAGCACAAGCGGGTTTACCAGTGCAATATCTGCAGCAAGATCTTCCAGAAC AGCAGCAACCTGAGCAGGCACGTGCGCTCGCATGGTGACAAGCTGTTTAAGTGCGAAGAGTGTGCAAAAT TGTTCAGCCGCAAAGAGAGCCTAAAGCAGCACGTTTCCTACAAGCACAGCAGGAACGAGGTGGACGGCGA GTACAGGTACCGCTGCGGCACTTGTGAGAAGACCTTCCGCATCGAGAGCGCGCTGGAGTTCCACAACTGC AGGACAGGACTCATAGCACACCCTGGAGAGGGGGGGCCCGGCGGCAGTCGGCTTAGAGACCTACCAGATG ACAAGACGTTCCAATGTGAGATGTGTTTCAGATTCTTCTCCACCAACAGCAACCTCTCCAAGCACAAGAA GAAGCACGGCGACAAGAAGTTTGCCTGTGAGGTCTGCAGCAAGATGTTCTACCGCAAGGACGTCATGCTG GACCACCAGCGCCGGCACCTGGAAGGAGTGCGGCGAGTGAAGCGAGAGGACCTGGAGGCCGGTGGGGAGA ACCTGGTCCGTTACAAGAAGGAGCCTTCCGGGTGCCCGGTGTGTGGCAAGGTGTTCTCCTGCCGGAGCAA TATGAACAAGCACCTGCTCACCCACGGCGACAAGAAGTACACCTGCGAGATCTGCGGGCGCAAGTTCTTC CGCGTGGATGTGCTCAGGGACCACATCCATGTCCACTTCAAGGACATCGCGTTGATGGATGACCACCAGA GGGAAGAGTTTATCGGCAAGATCGGGATCTCCTCGGAAGAAAACGATGACAATTCTGACGAGAGCGCAGA CTCGGAGCCTCACAAGTACAGCTGCAAGCGGTGCCAGCTCACCTTCGGCCGGGGGAAGGAGTACCTGAAG CACATCATGGAGGTGCACAAGGAGAAGGGCTATGGCTGCAGCATCTGCAACCGGCGCTTTGCACTGAAGG CCACCTACCACGCCCACATGGTCATCCACCGTGAAAACCTGCCGGACCCCAACGTGCAGAAGTACATCCA CCCCTGCGAGATCTGCGGGCGGATCTTCAACAGCATCGGGAACCTGGAGCGCCACAAGCTCATCCACACA GGTGTGAAGAGCCACGCCTGCGAGCAGTGTGGGAAGTCCTTTGCCAGGAAGGACATGCTGAAGGAGCACA TGCGTGTGCACGACAATGTCCGCGAGTACCTGTGTGCCGAGTGTGGGAAAGGCATGAAGACCAAGCACGC GCTGCGCCACCACATGAAGCTGCACAAGGGCATCAAGGAGTACGAGTGCAAGGAGTGCCACCGCAGGTTC GCGCAGAAGGTCAACATGCTCAAGCACTGCAAGCGGCACACGGGGATTAAAGATTTCATGTGTGAATTGT GTGGGAAGACATTCAGCGAGAGGAACACCATGGAGACCCACAAGCTCATCCACACAGTGGGCAAGCAGTG GACGTGCTCCGTGTGCGACAAGAAGTACGTGACCGAGTACATGCTGCAGAAGCACGTTCAGCTCACACAC GACAAGGTGGAGGCGCAGAGCTGCCAGCTGTGCGGGACCAAGGTGTCCACCAGGGCCTCCATGAGCCGAC ACATGCGGCGCAAGCACCCCGAGGTGCTCGCGGTGAGGATCGATGACCTGGACCACCTCCCGGAGACCAC CACCATCGACGCCTCCTCCATTGGCATCGTCCAGCCTGAGCTGACTCTGGAGCAGGAGGATTTGGCCGAA GGGAAGCACGGGAAAGCTGCCAAGCGAAGTCACAAGAGAAAGCAGAAGCCAGAAGAGGAGGCGGGTGCTC CGGTGCCCGAGGACGCCACCTTCAGCGAATACTCAGAGAAAGAGACGGAGTTCACAGGCAGTGTAGGCGA CGAGACCAATTCCGCAGTACAGAGCATTCAGCAGGTAGTGGTGACCCTGGGTGACCCAAATGTGACCACA CCATCGAGCTCAGTCGGCTTAACCAACATCACCGTGACCCCCATCACCACTGCGGCCGCGACTCAGTTTA CCAATCTCCAGCCGGTGGCCGTGGGGCACCTTACCACCCCTGAACGCCAGTTACAGCTGGACAACTCAAT CCTGACCGTGACCTTTGATACCGTCAGCGGCTCTGCCATGTTGCACAACCGCCAAAATGACGTCCAGATC CACCCCCAGCCGGAAGCCTCGAACCCACAGTCTGTGGCCCATTTCATCAACCTGACGACCCTGGTCAACT CCATCACGCCCCTGGGGAGCCAGCTTAGTGACCAGCACCCGCTCACGTGGCGGGCAGTGCCCCAGACTGA CGTCTTGCCACCCTCGCAGCCGCAGGCACCCCCACAGCAGGCGGCCCAGCCCCAGGTGCAGGCGGAGCAG CAGCAGCAGCAGATGTACAGCTACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001282934
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282934.1, NP_001269863.1
RefSeq Size	6742 bp
RefSeq ORF	3597 bp
Locus ID	63977
UniProt ID	P57071
Gene Summary	Sequence-specific DNA-binding transcriptional regulator. Plays a role as a molecular node in a transcriptional network regulating embryonic development and cell fate decision. Stimulates the expression of upstream key transcriptional activators and repressors of the Wnt/beta-catenin and MAPK/ERK pathways, respectively, that are essential for naive pluripotency and self-renewal maintenance of embryonic stem cells (ESCs). Specifically promotes SPRY1 and RSPO1 transcription activation through recognition and direct binding of a specific DNA sequence in their promoter regions. Involved in early embryo development (By similarity). Plays also a role in induced pluripotent stem cells (iPSCs) reprogramming (PubMed:28740264).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks seven alternate in-frame exons, contains an alternate in-frame exon, and differs in the 3' UTR, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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