RC3H1 (NM_001300850) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FHL6; IMDSHY; RNF198; ROQUIN
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001300850, the custom clone sequence may differ by one or more nucleotides ATGCCTGTACAAGCTCCACAATGGACGGATTTCCTCTCCTGCCCAATTTGCACTCAGACTTTCGACGAAA CAATTCGAAAGCCCATCAGTTTGGGTTGTGGCCATACTGTCTGCAAGATGTGCCTGAATAAACTCCACCG CAAGGCTTGCCCATTTGACCAGACCACTATCAATACAGACATTGAGCTCCTCCCTGTGAACTCAGCATTG CTGCAGCTCGTGGGTGCTCAGGTCCCTGAGCAGCAGCCTATTACTTTGTGTAGTGGGGTTGAAGACACAA AGCATTATGAGGAAGCCAAGAAATGTGTAGAAGAATTAGCATTGTACTTAAAACCGCTCAGCAGTGCTAG AGGAGTGGGTCTGAACAGCACTACTCAGAGTGTTCTGAGTCGCCCAATGCAGAGGAAGCTTGTGACTCTA GTCCACTGTCAACTAGTAGAAGAAGAAGGCAGGATTCGTGCCATGAGGGCAGCTCGATCTTTAGGTGAAC GAACAGTTACAGAGCTCATTCTCCAGCACCAGAATCCTCAGCAACTCTCTTCCAATCTTTGGGCAGCAGT AAGGGCTAGGGGATGCCAGTTCCTTGGACCAGCAATGCAGGAGGAAGCTTTAAAGTTGGTTCTGCTGGCC TTAGAAGATGGTTCTGCTTTGTCAAGAAAAGTATTGGTTCTGTTTGTGGTGCAAAGATTGGAGCCACGGT TTCCTCAAGCCTCTAAAACTAGCATTGGGCATGTTGTTCAGCTCCTTTATAGAGCCTCCTGTTTCAAGGT CACCAAACGTGATGAAGACTCTTCTTTGATGCAGCTGAAAGAAGAATTTAGAACCTATGAAGCTCTGCGG CGAGAACATGACTCCCAGATAGTGCAGATTGCTATGGAAGCAGGCTTACGAATTGCACCGGACCAGTGGT CCTCTTTGCTTTATGGAGACCAGTCTCACAAATCTCATATGCAGTCCATTATTGACAAGTTGCAGACTCC AGCCTCTTTTGCACAGAGTGTTCAGGAACTAACAATTGCTCTCCAGCGAACTGGAGACCCAGCAAACTTG AACCGACTAAGACCCCATTTGGAGCTGTTAGCAAACATTGACCCTAGTCCAGATGCTCCTCCTCCTACTT GGGAACAGCTGGAAAATGGGCTGGTTGCTGTGCGTACAGTGGTACATGGGCTGGTTGATTATATCCAGAA CCACAGCAAAAAAGGAGCAGATCAGCAGCAGCCTCCACAGCATAGCAAATACAAAACATACATGTGTCGA GATATGAAGCAGAGAGGAGGATGCCCTCGTGGGGCCAGCTGTACATTTGCACACTCACAGGAGGAACTGG AAAAATTTCGTAAAATGAATAAGCGCCTGGTTCCGAGAAGACCCTTGAGTGCCTCTTTGGGTCAACTTAA TGAGGTGGGCCTGCCTTCAGCAGCTATCCTTCCAGATGAAGGTGCAGTGGATCTCCCTAGCAGAAAACCT CCTGCTCTGCCAAATGGAATTGTATCAACAGGGAATACAGTAACACAGCTTATTCCGCGAGGGACAGACC CCAGCTATGATTCTAGTCTGAAACCAGGAAAAATAGATCATCTGAGCAGTAGTGCTCCTGGATCCCCTCC TGACCTGCTAGAATCTGTTCCTAAGAGTATTTCTGCCTTACCTGTGAATCCACATTCTATACCTCCAAGG GGGCCAGCAGATCTGCCTCCAATGCCTGTTACCAAACCACTTCAGATGGTACCTCGAGGTTCTCAGTTAT ATCCAGCACAACAGACGGATGTTTATTATCAGGATCCTCGAGGAGCAGCTCCGCCATTTGAACCAGCACC TTATCAGCAGGGTATGTATTATACTCCACCACCACAATGTGTGTCCCGCTTTGTCCGACCTCCACCATCT GCTCCTGAACCTGCTCCTCCCTACTTGGATCATTATCCACCCTACCTCCAAGAACGTGTTGTAAACTCTC AGTATGGCACACAGCCACAGCAGTACCCACCTATATACCCATCTCACTATGATGGCCGTCGAGTGTACCC TGCTCCGTCTTACACAAGAGAAGAGATATTCCGAGAAAGCCCTATACCCATTGAGATTCCACCTGCAGCA GTACCATCGTATGTACCAGAATCCAGAGAAAGATACCAACAGATCGAGAGTTACTATCCAGTGGCTCCTC ATCCAACTCAGATCAGACCTTCGTACCTCAGAGAACCTCCTTATAGCCGGCTTCCTCCTCCTCCCCAGCC TCATCCTAGTCTAGATGAACTACATCGCCGACGAAAGGAAATAATGGCCCAGCTAGAGGAAAGAAAGGTT ATCTCTCCACCTCCTTTTGCACCTTCACCAACCTTGCCTCCTACCTTTCATCCGGAAGAATTTTTGGATG AAGACTTGAAGGTAGCTGGGAAATACAAAGGAAATGATTATAGCCAATACTCTCCCTGGTCATGTGACAC CATCGGCTCCTACATTGGAACCAAAGATGCAAAACCCAAAGATGTTGTGGCAGCAGGGAGTGTGGAAATG ATGAATGTGGAGAGTAAAGGAATGAGGGACCAGCGATTAGATCTTCAGAGAAGAGCAGCAGAAACCAGTG ATGATGACCTCATCCCATTTGGAGACCGACCAACAGTGTCTCGGTTTGGTGCCATCTCACGAACTTCCAA AACTATATATCAGGGTGCTGGTCCAATGCAGGCTATGGCACCTCAGGGAGCTCCTACAAAATCTATTAAC ATTTCAGATTATAGTCCATATGGAACCCACGGTGGCTGGGGAGCTTCTCCATATTCACCTCATCAAAACA TACCTTCTCAGGGACACTTCAGTGAGAGGGAGAGAATATCTATGTCAGAAGTGGCCAGTCATGGAAAACC CCTTCCATCTGCTGAGAGAGAACAGCTACGACTAGAATTGCAGCAATTGAACCATCAGATTAGCCAGCAG ACCCAGCTACGTGGACTAGAGGCTGTTAGTAACAGGCTGGTGTTGCAGAGGGAGGCAAACACCCTGGCAG GCCAGTCACAGCCCCCACCACCGCCACCTCCAAAATGGCCTGGGATGATCTCAAGTGAGCAGTTGAGCTT GGAACTGCACCAGGTGGAAAGGGAAATCGGGAAGAGAACACGGGAACTGAGTATGGAAAACCAGTGTTCT CTGGACATGAAAAGCAAACTGAATACAAGTAAACAAGCAGAAAATGGACAACCAGAACCACAAAACAAGG TTCCGGCTGAGGACCTTACATTGACATTCAGCAGTGATGTACCAAATGGATCAGCCTTGACACAAGAGAA TATCAGCCTCCTATCAAACAAGACCAGCTCTCTGAACCTGTCAGAGGACCCTGAGGGAGGAGGGGATAAT AATGACTCCCAGAGATCAGGAGTTACTCCCAGTTCTGCTCCTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001300850
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001300850.1, NP_001287779.1
RefSeq Size	11129 bp
RefSeq ORF	3405 bp
Locus ID	149041
UniProt ID	Q5TC82
Protein Families	Druggable Genome
Gene Summary	This gene encodes a protein containing RING-type and C3H1-type zinc finger motifs. The encoded protein recognizes and binds to a constitutive decay element (CDE) in the 3' UTR of mRNAs, leading to mRNA deadenylation and degradation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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