PLCH2 (NM_001303012) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PLC-eta2; PLC-L4; PLCeta2; PLCL4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001303012, the custom clone sequence may differ by one or more nucleotides ATGGAAGAGCCTGGGCCCCCAGGTGGGCTCAGCCAAGACCAAGTGGAGCGGTGCATGGGTGCCATGCAAG AGGGGATGCAGATGGTGAAGCTGCGTGGCGGCTCCAAGGGCCTGGTCCGCTTCTACTACCTGGACGAGCA CCGCTCCTGCATCCGCTGGAGGCCCTCACGCAAGAACGAGAAGGCCAAGATCTCCATCGACTCCATCCAG GAGGTGAGTGAGGGGCGGCAGTCGGAGGTCTTCCAGCGCTACCCTGACGGCAGCTTCGACCCCAACTGCT GCTTCAGCATCTACCACGGCAGCCACCGCGAGTCGCTGGACCTGGTCTCCACCAGCAGCGAGGTGGCGCG CACCTGGGTCACTGGCCTGCGCTACCTCATGGCCGGCATCAGCGACGAGGACAGCCTGGCTCGCCGCCAG CGCACCAGGGACCAGTGGCTGAAGCAGACGTTTGACGAGGCCGACAAGAACGGGGATGGCAGCCTGAGCA TTGGCGAGGTCCTGCAGCTGCTGCACAAGCTCAACGTGAACCTGCCCCGGCAGAGGGTGAAGCAGATGTT CAGGGAAGCGGACACGGATGACCACCAAGGGACGCTGGGTTTTGAAGAGTTCTGTGCCTTCTACAAGATG ATGTCCACCCGCCGGGACCTCTACCTGCTCATGCTGACCTACAGCAACCACAAGGACCACCTGGATGCCG CCAGCCTGCAGCGCTTCCTGCAGGTGGAGCAGAAGATGGCGGGTGTGACCCTCGAGAGCTGCCAGGACAT CATCGAGCAGTTTGAGCCATGCCCAGAAAACAAGAGTAAGGGGCTGCTGGGCATTGATGGCTTCACCAAC TACACCAGGAGCCCTGCTGGTGACATCTTCAACCCTGAGCACCACCATGTGCACCAGGACATGACGCAGC CGCTGAGCCACTACTTCATCACCTCGTCCCACAACACCTACCTCGTGGGTGACCAGCTCATGTCCCAGTC ACGGGTGGACATGTATGCTTGGGTCCTGCAGGCTGGCTGCCGCTGCGTGGAGGTGGACTGCTGGGATGGG CCCGACGGGGAGCCCATTGTGCACCATGGCTACACTCTGACTTCCAAGATCCTCTTCAAAGACGTCATTG AAACCATCAACAAATATGCCTTCATCAAGAATGAGTACCCAGTGATCCTGTCCATCGAAAACCACTGCAG TGTCATCCAGCAGAAGAAAATGGCCCAGTATCTGACTGACATCCTTGGGGACAAGCTGGACCTGTCATCA GTGAGCAGTGAAGATGCCACCACACTCCCCTCTCCACAGATGCTCAAGGGCAAGATCCTCGTGAAGGGGA AGAAGCTCCCAGCCAACATCAGCGAGGATGCGGAGGAAGGCGAGGTGTCTGATGAGGACAGTGCTGATGA GATTGACGATGACTGCAAGCTCCTCAATGGGGATGCATCCACCAATCGAAAGCGTGTAGAAAACACTGCT AAGAGGAAACTGGATTCCCTCATCAAAGAGTCGAAGATTCGGGACTGTGAGGACCCCAACAACTTCTCCG TCTCCACACTGTCCCCATCTGGAAAGCTCGGACGCAAGAGCAAGGCTGAAGAGGACGTGGAGTCTGGGGA GGATGCCGGGGCCAGCAGACGCAATGGCCGCCTCGTCGTGGGAAGCTTCTCCAGGCGCAAGAAGAAGGGC AGCAAGCTGAAGAAGGCGGCCAGCGTGGAGGAGGGAGATGAGGGTCAGGACTCCCCGGGAGGCCAGAGCC GAGGGGCGACCCGGCAGAAGAAGACCATGAAGCTGTCCCGGGCCCTCTCTGACCTGGTGAAGTACACCAA GTCCGTGGCCACCCACGACATAGAGATGGAGGCGGCGTCCAGCTGGCAGGTGTCGTCCTTCAGCGAGACC AAGGCCCACCAGATTCTGCAGCAGAAGCCGGCGCAGTACCTACGCTTCAACCAGCAGCAGCTCTCCCGCA TCTACCCCTCCTCCTACCGTGTGGACTCCAGCAACTACAACCCGCAGCCCTTCTGGAACGCCGGCTGCCA AATGGTTGCCCTGAACTACCAGTCAGAGGGGCGGATGCTGCAGCTGAACCGAGCCAAGTTCAGCGCCAAC GGTGGCTGCGGCTACGTACTCAAGCCTGGGTGCATGTGCCAGGGCGTGTTCAACCCCAACTCGGAGGACC CCCTGCCCGGGCAGCTCAAGAAGCAGCTGGTGCTCCGGATCATCAGTGGCCAGCAGCTTCCCAAGCCGCG CGACTCCATGCTGGGGGACCGTGGGGAGATCATCGACCCCTTTGTGGAGGTGGAGATCATTGGGCTCCCT GTGGACTGCAGCAGGGAGCAGACCCGCGTGGTGGACGACAACGGGTTCAACCCCACCTGGGAGGAGACCC TGGTTTTCATGGTGCACATGCCGGAGATCGCGCTGGTCCGCTTCCTCGTCTGGGACCACGATCCCATCGG GCGTGACTTCATTGGCCAGAGGACGCTGGCCTTCAGCAGCATGATGCCAGGCTACAGACACGTGTACCTA GAAGGGATGGAAGAGGCCTCCATCTTCGTGCATGTGGCTGTCAGTGACATCAGCGGTAAGGTCAAGCAGG CTCTGGGCCTAAAAGGCCTCTTCCTCCGAGGCCCAAAGCCCGGCTCGCTGGACAGTCATGCTGCTGGGCG GCCCCCGGCCCGGCCCTCCGTTAGCCAGCGGATCCTGCGGCGCACGGCCAGCGCCCCGACCAAGAGCCAG AAGCCGGGCCGCAGGGGCTTCCCGGAGCTGGTCCTGGGTACACGGGACACAGGCTCCAAGGGGGTGGCAG ACGATGTGGTGCCCCCCGGGCCCGGACCTGCTCCGGAAGCCCCAGCCCAGGAGGGGCCCGGCAGCGGCAG CCCCCGAGGTAAGGCGCCAGCTGCGGTGGCAGAGAAGAGCCCTGTGCGAGTGCGGCCCCCGCGTGTCCTG GACGGCCCCGGGCCTGCTGGGATGGCCGCCACATGCATGAAGTGTGTGGTGGGATCCTGCGCCGGCGTGA ACACCGGGGGCCTGCAGAGGGAGCGGCCACCCAGCCCGGGGCCTGCAAGCAGGCAGGCAGCCATTCGCCA GCAGCCCCGGGCCCGGGCTGACTCACTGGGGGCCCCCTGCTGTGGCCTGGACCCTCACGCTATCCCGGGG AGAAGCAGAGAGGCCCCCAAGGGTCCTGGGGCCTGGAGGCAGGGTCCAGGCGGTAGCGGCTCCATGTCCT CGGACTCCAGCAGCCCAGACAGCCCGGGCATCCCCGAAAGGTCCCCCCGCTGGCCTGAGGGTGCCTGCAG GCAACCGGGGGCCCTGCAGGGAGAGATGAGTGCCTTGTTTGCTCAAAAGCTGGAGGAGATCAGGAGTAAA TCCCCCATGTTCTCCGCCGTTAGGAACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001303012
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001303012.1, NP_001289941.1
RefSeq Size	5472 bp
RefSeq ORF	3390 bp
Locus ID	9651
UniProt ID	O75038
Protein Families	Druggable Genome
Gene Summary	PLCH2 is a member of the PLC-eta family of the phosphoinositide-specific phospholipase C (PLC) superfamily of enzymes that cleave PtdIns(4,5) P2 to generate second messengers inositol 1,4,5-trisphosphate and diacylglycerol (Zhou et al., 2005 [PubMed 16107206]).[supplied by OMIM, Jun 2009] Transcript Variant: This variant (2) contains an alternate 5'-terminal exon, uses an alternate start codon, and uses an alternate splice site in the 3' coding region, which results in a frameshift compared to variant 1. The encoded isoform (2) has shorter and distinct N- and C-termini, compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.