IFT122 (NM_001280545) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CED; CED1; FAP80; SPG; WDR10; WDR10p; WDR140
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001280545, the custom clone sequence may differ by one or more nucleotides ATGTTCAATGGGATCATCAGCATACGGAACAAAAATGGCGAGGAGAAAGTAAAGATCGAGCGGCCGGGGG GCTCCCTCTCGCCAATATGGTCCATCTGCTGGAACCCTTCAAGCCGATGGGAGAGTTTCTGGATGAACAG AGAGAATGAGGATGCCGAGGATGTCATTGTCAACAGATATATTCAGGAAATCCCTTCCACTCTGAAGTCA GCAGTGTACAGTAGTCAGGGTAGTGAGGCAGAGGAGGAAGAACCAGAGGAAGAGGACGACAGTCCCAGGG ACGACAACTTAGAGGAACGTAATGACATCCTGGCTGTGGCTGACTGGGGACAGAAAGTTTCCTTCTACCA GCTGAGTGGAAAACAGATTGGAAAGGATCGGGCACTGAACTTTGACCCCTGCTGCATCAGCTACTTTACT AAAGGCGAGTACATTTTGCTGGGGGGTTCAGACAAGCAAGTATCTCTTTTCACCAAGGATGGAGTGCGGC TTGGGACTGTTGGGGAGCAGAACTCCTGGGTGTGGACGTGTCAAGCGAAACCGGATTCCAACTATGTGGT GGTCGGCTGCCAGGACGGCACCATTTCCTTCTACCAGCTTATTTTCAGCACAGTCCATGGGCTTTACAAG GACCGCTATGCCTACAGGGATAGCATGACTGACGTCATTGTGCAGCACCTGATCACTGAGCAGAAAGTTC GGATTAAATGCAAAGAGCTTGTCAAGAAGATTGCCATCTACAGAAATCGATTGGCTATCCAACTGCCAGA GAAAATCCTCATCTATGAGTTGTATTCAGAGGACTTATCAGACATGCATTACCGGGTAAAGGAGAAGATT ATCAAGAAGTTTGAGTGCAACCTCCTGGTGGTGTGTGCCAATCACATCATCCTGTGCCAGGAGAAACGGC TGCAGTGCCTGTCCTTCAGCGGAGTGAAGGAGCGGGAGTGGCAGATGGAGTCTCTCATTCGTTACATCAA GGTGATCGGTGGCCCTCCTGGAAGAGAAGGCCTCTTAGTGGGGCTGAAGAATGGACAGATCCTGAAGATC TTCGTGGACAATCTCTTTGCTATCGTCCTGCTGAAGCAGGCCACAGCTGTGCGCTGCTTGGACATGAGTG CCTCCCGTAAGAAGCTGGCCGTGGTAGATGAAAATGACACTTGCCTGGTGTATGACATCGACACCAAGGA GCTGCTTTTTCAGGAACCAAACGCCAACAGTGTAGCTTGGAACACCCAGTGTGAGGACATGCTCTGCTTC TCGGGAGGAGGCTACCTCAACATCAAAGCCAGCACCTTCCCTGTGCACCGGCAGAAGCTGCAGGGCTTTG TGGTCGGCTACAATGGCTCCAAGATCTTCTGCCTCCATGTCTTCTCCATTTCTGCCGTGGAGGTGCCGCA GTCCGCTCCCATGTACCAGTACCTGGATAGGAAACTGTTCAAGGAAGCCTACCAGATTGCTTGCTTGGGT GTCACAGACACTGATTGGCGTGAACTGGCCATGGAAGCGCTAGAAGGTTTAGATTTTGAAACAGCAAAGA AGGCCTTCATCAGAGTACAAGACCTCCGATATTTAGAGCTCATCAGCAGCATTGAGGAGAGGAAGAAGCG GGGAGAGACCAACAATGACCTGTTTCTGGCAGATGTGTTTTCCTACCAGGGGAAGTTCCATGAGGCCGCC AAACTGTACAAGAGGAGTGGGCACGAGAACCTCGCGCTTGAAATGTACACCGACCTCTGCATGTTTGAGT ATGCCAAGGATTTCCTTGGATCTGGAGACCCCAAAGAAACAAAGATGCTAATCACCAAACAGGCTGACTG GGCCAGAAATATCAAGGAGCCCAAAGCCGCCGTGGAGATGTACATCTCAGCAGGAGAGCACGTCAAGGCC ATCGAGATCTGTGGTGACCATGGCTGGGTTGACATGTTGATCGACATCGCCCGCAAACTGGACAAGGCTG AGCGCGAGCCCCTGCTGCTGTGCGCTACCTACCTCAAGAAGCTGGACAGCCCTGGCTATGCTGCTGAGAC CTACCTGAAGATGGGTGACCTCAAGTCCCTGGTGCAGCTGCACGTGGAGACCCAGCGCTGGGATGAGGCC TTTGCTTTGGGTGAGAAGCATCCTGAGTTTAAGGATGACATCTACATGCCGTATGCTCAGTGGCTAGCAG AGAACGATCGCTTTGAGGAAGCCCAGAAAGCGTTCCACAAGGCTGGGCGACAGAGAGAAGCGGTCCAGGT GCTGGAGCAGCTCACAAACAATGCCGTGGCGGAGAGCAGGTTTAATGATGCTGCCTATTATTACTGGATG CTGTCCATGCAGTGCCTCGATATAGCTCAAGATCCTGCCCAGAAGGACACAATGCTTGGCAAGTTCTACC ACTTCCAGCGTTTGGCAGAGCTGTACCATGGTTACCATGCCATCCATCGCCACACGGAAGATCCGTTCAG TGTCCATCGTCCTGAAACTCTTTTCAACATCTCCAGGTTCCTGCTGCACAGCCTGCCCAAGGACACCCCC TCGGGCATCTCTAAAGTGAAAATACTCTTCACCTTGGCCAAGCAGAGCAAGGCCCTCGGTGCCTACAGGC TGGCCCGGCACGCCTATGACAAGCTGCGTGGCCTGTACATCCCTGCCAGATTCCAAAAGTCCATTGAGCT GGGTACCCTGACCATCCGCGCCAAGCCCTTCCACGACAGTGAGGAGTTGGTGCCCTTGTGCTACCGCTGC TCCACCAACAACCCGCTGCTCAACAACCTGGGCAACGTCTGCATCAACTGCCGCCAGCCCTTCATCTTCT CCGCCTCTTCCTACGACGTGCTACACCTGGTTGAGTTCTACCTGGAGGAAGGGATCACTGATGAAGAAGC CATCTCCCTCATCGACCTGGAGGTGCTGAGACCCAAGCGGGATGACAGACAGCTAGAGATTGCAAACAAC AGCTCCCAGATTCTGCGGCTAGTGGAGACCAAGGACTCCATCGGAGATGAGGACCCGTTCACAGCTAAGC TGAGCTTTGAGCAAGGTGGCTCAGAGTTCGTGCCAGTGGTGGTGAGCCGGCTGGTGCTGCGCTCCATGAG CCGCCGGGATGTCCTCATCAAGCGATGGCCCCCACCCCTGAGGTGGCAATACTTCCGCTCACTGCTGCCT GACGCCTCCATTACCATGTGCCCCTCCTGCTTCCAGATGTTCCATTCTGAGGACTATGAGTTGCTGGTGC TTCAGCATGGCTGCTGCCCCTACTGCCGCAGGTGCAAGGATGACCCTGGCCCATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001280545
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001280545.1, NP_001267474.1
RefSeq Size	4414 bp
RefSeq ORF	3276 bp
Locus ID	55764
UniProt ID	Q9HBG6
Gene Summary	This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This cytoplasmic protein contains seven WD repeats and an AF-2 domain which function by recruiting coregulatory molecules and in transcriptional activation. Mutations in this gene cause cranioectodermal dysplasia-1. A related pseudogene is located on chromosome 3. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (6) lacks an alternate exon and contains an alternate exon in the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 6, which is shorter than isoform 1.

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