RGS22 (NM_001286693) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CT145; PRTD-NY2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001286693, the custom clone sequence may differ by one or more nucleotides ATGAATGCCCCCGATGAAGATGAGACCATTAATGTCAACTACAATATTATGGCTTCCTATACTCAAACAA AAGATTGGTTTGCATTAGCAAAACAAAGTCAGCAAACAGTATCAACCTTTTCGTTACCCTGTTGTGTACC CTACAACAAACTTAAATCACCAGCTATTTCATCTGTTTCTGAGAATTTTATCTTTGATGATGGAGTTCAC CCTAGGACAAAAAAGGACCCATCTAAAACCAACAAATTGATTTCTGAATTTGAAGAAGAAGAAGGAGAAG AAGAAGAGGTGTCTGTATCTCTACAAGACACTCCTTCTCAAGCTCTTCTGAGAGTATACCTTGAAAAGAA ACAGGATGTTGATGAAAGCCTGACAATGCATTTCTCAACATGTGAAGAATTTTTAAGCTCCTATATATAC TTTATTTTGAGAGGAGCAATTCAGCAAATTGTTGGAAAGCCAGTTGGAGAAACCCCAGACTATATAAACT TCAACAATATAACAAAAGTGTCATTTGATGATTGTTTTGAGTCTATTCATGGCAAGAATTTTTTAAGTGA ATTAGTTCAAACTACAAAGGAGAGGTCAGAAGAGATAGAACAAACAAGTTTAAGTTCAAAGAATGAGAGC GCTGGACCAGAGAGCAGGGCGGACTGGTGTATTTCTCATAGGACTTATGACATTGGCAATAGAAAGGAGT TTGAAAGATTTAAGAAATTTATAAAAGGTACATTGGGAGAGAGATATTGGTGGCTATGGATGGATATTGA GAGGTTAAAAGTTCTTAAGGATCCTGGAAGACATCAAAGACATCTTGAGAAGATGAAAAAATGCTATCTA GTGAGCAATGGAGATTACTACCTTTCTGCAGAAATCTTATCAAAATTTAAACTGTTAGATGGATCACAGT GGAATGAAGAGCATTTAAGAAATATTCAGTCTGAAGTTTTAAAACCTTTACTTCTGTATTGGGCACCAAG ATTCTGTGTTACTCATTCAGCCTCAACAAAATATGCTAGTGCTGAACTGAAATTCTGGCACCTCCGTCAA GCAAAACCAAGGAAGGATATTGACCCTTTTCCACAAATGGCAACCCTCTTGCCATTAAGACCCAAATCTT GCATTCCACAGATACCTGAGATCCAGAAAGAAGAATTCAGCTTATCACAACCTCCTAAATCTCCCAATAA ATCACCTGAAGTAAAAACAGCAACTCAAAAGCCTTGGAAGCGGGAGCTTTTGTATCCAGGTTCTTCTAAG GATGATGTGATTGAGAAAGGGTCAAAGTACATGTCAGAAAGCAGCAAAGTCATTCATTTAACATCTTTTA CTGACATTTCTGAATGTCTCAAGCCCCAGCTGGATAGAAGATACGCTTATACAGAAGAACCTAGGGTTAA AACCGTGTCAGATGTTGGTGCCTTGGGAGGATCTGACATGGAAAATTTGTTGCAATCTTTGTATGTAGAA AATAGAGCTGGATTCTTCTTTACTAAATTCTGCGAGCATTCAGGGAACAAGTTGTGGAAGAACAGTGTGT ACTTTTGGTTTGACCTGCAGGCTTATCATCAGCTTTTCTACCAAGAAACACTTCAGCCTTTTAAAGTATG CAAGCAAGCGCAATATCTTTTTGCCACATACGTTGCTCCTTCTGCCACTCTTGACATTGGACTCCAACAG GAAAAGAAAAAAGAAATTTATATGAAAATACAGCCACCATTTGAAGACCTTTTTGACACAGCAGAGGAAT ATATCCTTCTCCTCCTTCTTGAGCCATGGACAAAGATGGTAAAATCGGACCAAATTGCTTATAAAAAGGT GGAGCTGGTGGAAGAAACTCGACAGTTAGACTCCACATACTTCAGAAAGCTACAGGCTTTGCATAAAGAG ACATTTTCCAAGAAAGCTGAGGACACCACTTGTGAAATTGGAACTGGCATTTTATCACTCTCTAACGTCT CTAAACGAACAGAATATTGGGATAATGTTCCTGCAGAATACAAGCATTTTAAGTTTAGTGATCTGCTTAA CAACAAACTGGAGTTTGAACATTTCCGTCAGTTTCTTGAGACTCATTCTTCAAGCATGGATCTTATGTGC TGGACAGACATTGAGCAGTTCCGGAGAATAACTTACAGAGATCGAAATCAAAGGAAGGCAAAATCTATAT ACATTAAAAACAAATACCTTAATAAAAAATATTTCTTTGGACCCAACAGTCCAGCTTCTCTGTATCAGCA GAACCAGGTAATGCATTTAAGTGGTGGCTGGGGGAAGATTCTTCATGAGCAGCTTGATGCACCTGTTCTA GTTGAAATCCAGAAGCATGTGCAAAATAGGCTAGAAAATGTATGGCTGCCATTGTTTCTTGCAAGTGAAC AGTTTGCAGCACGTCAGAAGATAAAGGTACAGATGAAAGACATAGCAGAAGAGCTCTTACTACAGAAGGC TGAAAAGAAAATCGGGGTCTGGAAGCCTGTGGAGAGTAAGTGGATCTCTTCATCTTGTAAAATCATTGCT TTTCGCAAAGCATTATTGAATCCAGTTACTTCAAGACAATTTCAACGTTTTGTGGCTCTAAAAGGAGATT TATTGGAAAATGGTTTACTCTTTTGGCAAGAAGTACAAAAATATAAGGACTTGTGCCATTCTCATTGTGA TGAGTCTGTCATCCAGAAGAAGATTACAACTATTATCAACTGCTTTATTAATTCCAGTATTCCACCAGCT TTACAAATTGACATTCCAGTAGAGCAAGCCCAGAAGATTATTGAACACCGGAAGGAGTTAGGACCATATG TATTTAGAGAGGCACAGATGACAATTTTTGGGGTTCTGTTTAAATTCTGGCCTCAGTTCTGTGAGTTTAG GAAGAATTTAACAGATGAAAATATTATGAGTGTTTTAGAGAGAAGACAAGAATATAATAAGCAGAAAAAA AAATTGGCAGTCCTAGAAGACGAAAAATCTGGAAAGGATGGAATCAAACAATATGCAAATACTTCAGTGC CTGCTATCAAAACTGCTTTACTCAGTGATTCCTTCCTAGGCCTCCAACCATATGGCCGACAGCCAACCTG GTGCTACTCAAAGTATATAGAAGCCTTAGAACAGGAGAGAATTCTTCTTAAGATCCAAGAAGAACTAGAA AAAAAGTTGTTTGCAGGCTTGCAACCTCTCACAAATTTTAAGGCTAGCTCTTCAACTATGTCTTTGAAAA AGAATATGTCTGCCCACAGCAGCCAGAAGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001286693
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286693.1, NP_001273622.1
RefSeq Size	4169 bp
RefSeq ORF	3252 bp
Locus ID	26166
Gene Summary	Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus, compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.