ZRANB3 (NM_001286568) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	4933425L19Rik; AH2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001286568, the custom clone sequence may differ by one or more nucleotides ATGCCTAGGGTTCATAACATAAAAAAGTCTCTTACACCTCACATTTCTTGTGTGACAAATGAATCTGATA ATCTGCTGGATTTTTTGCCTGACAGACTAAGAGCAAAGCTACTTCCATTCCAGAAAGATGGCATCATTTT TGCCCTCAAAAGAAATGGCAGGTGTATGGTGGCTGATGAAATGGGTCTAGGAAAGACAATCCAGGCAATT GGAATTACTTACTTCTATAAAGAGGAATGGCCTCTGTTAATAGTGGTCCCTTCGTCTCTGAGGTACCCTT GGACAGAAGAAATTGAAAAATGGATCCCAGAGCTAAGTCCAGAAGAAATCAATGTTATTCAGAATAAAAC TGATGTTAGGAGAATGTCGACCAGTAAAGTGACAGTTCTGGGTTATGGTCTCTTAACCGCAGATGCAAAG ACTTTGATAGATGCACTGAATAATCAGAACTTCAAAGTAGTTATAGTGGATGAATCACACTACATGAAAT CCAGAAATGCAACTCGCAGCAGGATTTTATTGCCAATAGTACAGAAAGCCAGACGAGCCATTCTTCTTAC AGGAACACCAGCTTTAGGAAGGCCTGAAGAGCTTTTTATGCAGATTGAAGCTCTCTTTCCACAAAAATTT GGAAGATGGACCGACTATGCAAAAAGATACTGTAATGCACACATCAGATATTTTGGTAAAAGACCTCAGT GGGATTGTAGAGGGGCATCAAATCTTAATGAACTTCACCAGCTATTAAGTGACATAATGATTAGAAGATT AAAGACTGAAGTTTTAACCCAGCTACCCCCTAAAGTCAGACAGCGTATTCCATTTGATCTTCCATCAGCA GCTGCCAAGGAATTGAATACCAGCTTTGAAGAGTGGGAAAAAATAATGAGAACTCCAAATTCAGGTGCCA TGGAGACAGTCATGGGGTTGATAACTCGCATGTTTAAACAAACTGCTATTGCCAAGGCAGGTGCTGTAAA GGATTATATTAAGATGATGCTTCAGAATGATTCGCTTAAATTTCTGGTTTTTGCTCACCATTTAAGCATG CTCCAAGCTTGCACAGAAGCAGTCATCGAAAATAAGACTCGTTACATTAGGATAGATGGAAGTGTTTCAT CTTCAGAAAGAATACATCTGGTTAATCAGTTTCAAAAGGATCCTGACACTCGCGTGGCTATCCTAAGCAT TCAGGCTGCTGGCCAGGGATTAACATTTACTGCAGCAAGTCATGTTGTATTTGCTGAGTTGTACTGGGAC CCTGGACATATAAAACAAGCAGAAGACCGAGCTCACAGAATTGGCCAGTGCAGTTCTGTGAATATTCACT ACCTTATTGCAAATGGAACCCTAGACACCCTTATGTGGGGAATGTTGAATCGCAAGGCTCAAGTTACAGG GAGCACACTGAACGGTAGGAAAGAAAAAATTCAGGCTGAGGAAGGTGATAAGGAAAAATGGGATTTCCTG CAGTTTGCTGAAGCTTGGACTCCAAATGACAGTTCTGAAGAGTTAAGGAAGGAAGCTTTGTTCACTCACT TCGAAAAAGAAAAACAGCATGATATTCGATCATTTTTTGTACCACAACCTAAAAAAAGACAGTTGATGAC CTCCTGTGACGAATCAAAGAGATTCCGGGAGGAAAATACTGTAGTGTCATCAGACCCTACAAAAACAGCT GCAAGAGATATCATCGATTATGAAAGTGATGTTGAACCTGAAACGAAAAGATTGAAATTGGCTGCCTCGG AAGACCACTGCAGTCCGTCGGAAGAGACACCATCCCAGTCCAAGCAAATCCGAACTCCACTCGTGGAAAG TGTACAGGAGGCCAAGGCCCAGTTAACCACCCCAGCCTTTCCTGTAGAGGGCTGGCAATGTAGTCTCTGC ACCTATATCAATAATTCAGAGTTACCTTATTGTGAAATGTGTGAGACTCCTCAAGGCAGTGCTGTTATGC AAATAGATAGCCTCAACCATATCCAGGATAAAAACGAGAAGGATGATTCTCAGAAAGACACCTCCAAAAA GGTTCAAACTATCTCAGACTGTGAAAAACAAGCCCTTGCACAGTCAGAACCTGGCCAGTTGGCTGACAGC AAGGAAGAAACACCAAAAATTGAGAAAGAAGACGGACTTACATCCCAGCCAGAACAGTGGAAGAGTTCAG ACACTTTGCCAGTGTATGACACCTTAATGTTCTGTGCAAGTAGGAATACTGACCGGATTCACATCTATAC TAAGGATGGAAAACAGATGAGCTGTAATTTCATTCCTCTGGATATAAAATTAGACCTTTGGGAAGATTTA CCAGCAAGCTTTCAGCTGAAACAATATCGCTCACTGATTTTGAGATTTGTTCGAGAATGGAGTAGTCTAA CTGCCATGAAGCAAAGGATAATCAGGAAAAGTGGACAGCTATTCTGTAGCCCAATTCTTGCTTTGGAAGA GATCACAAAGCAACAAACCAAACAAAATTGCACCAAAAGATACATAACCAAAGAAGATGTTGCCGTAGCC TCAATGGACAAAGTGAAGAATGTTGGGGGCCATGTCCGTCTGATCACAAAGGAGTCCAGGCCACGGGATC CTTTCACAAAAAAACTTCTTGAAGATGGAGCCTGTGTCCCATTTCTAAATCCATACACAGTCCAGGCAGA TCTCACTGTGAAGCCCTCTACATCCAAAGGCTATTTGCAAGCTGTGGATAATGAAGGAAATCCACTTTGC CTTCGCTGTCAGCAACCCACTTGCCAGACTAAGCAAGCATGTAAAGCGAACTCTTGGGATTCACGGTTTT GCTCTCTGAAATGTCAGGAAGAGTTTTGGATTCGATCTAATAACAGTTACCTGAGAGCCAAAGTATTTGA AACTGAACATGGTGTGTGTCAGCTCTGTAATGTGAACGCACAAGAACTCTTTTTACGTCTGAGAGATGCC CCTAAAAGTCAGAGGAAGAATCTTCTGTATGCTACCTGGACTTCAAAGCTCCCATTAGAACAGCTAAATG AAATGATAAGAAACCCAGGGGAAGGACATTTCTGGCAGGTGGATCACATCAAGCCAGTGTATGGGGGAGG AGGACAGTGTTCCCTGGACAACCTGCAGACTCTCTGCACAGTCTGTCACAAAGAGAGAACTGCCAGACAA GCTAAGGAAAGAAGCCAGGTGAGAAGACAATCTCTAGCATCAAAGCATGGATCAGACATCACACGATTTT TGGTAAAGAAGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286568
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286568.1, NP_001273497.1
RefSeq Size	6829 bp
RefSeq ORF	3234 bp
Locus ID	84083
UniProt ID	Q5FWF4
Protein Families	Druggable Genome
Gene Summary	DNA annealing helicase and endonuclease required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could occur during template switching events (PubMed:21078962, PubMed:22704558, PubMed:22705370, PubMed:22759634, PubMed:26884333). Recruited to the sites of stalled DNA replication by polyubiquitinated PCNA and acts as a structure-specific endonuclease that cleaves the replication fork D-loop intermediate, generating an accessible 3'-OH group in the template of the leading strand, which is amenable to extension by DNA polymerase (PubMed:22759634). In addition to endonuclease activity, also catalyzes the fork regression via annealing helicase activity in order to prevent disintegration of the replication fork and the formation of double-strand breaks (PubMed:22705370, PubMed:22704558).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate splice site in the central coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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