GMIP (NM_001288999) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARHGAP46
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001288999, the custom clone sequence may differ by one or more nucleotides ATGGACGCAGCAGAGCCGGGACTCCCCCCAGGTCCTGAGGGCAGGAAGAGGTACAGTGACATCTTCCGGA GCCTGGACAACCTCGAAATCTCACTGGGGAACGTGACCCTTGAGATGCTGGCTGGAGACCCTCTACTCTC AGAAGACCCAGAACCTGACAAGACCCCTACAGCCACTGTTACCAACGAAGCCAGCTGTTGGAGCGGCCCC TCCCCAGAGGGTCCTGTACCCCTCACAGGGGAGGAACTGGACTTGCGGCTCATTCGGACAAAGGGGGGTG TGGACGCAGCCCTGGAATATGCCAAGACCTGGAGCCGCTATGCCAAGGAACTGCTTGCCTGGACTGAAAA GAGAGCCAGCTATGAGCTGGAGTTTGCTAAGAGCACCATGAAGATCGCTGAAGCTGGCAAGGTGTCCATT CAACAGCAGAGCCACATGCCTCTGCAGTACATCTACACCCTGTTTCTGGAGCACGATCTCAGCCTGGGAA CCCTGGCCATGGAGACAGTGGCCCAGCAGAAAAGAGACTACTACCAGCCCCTCGCCGCCAAACGGACTGA GATTGAGAAGTGGCGGAAGGAGTTCAAGGAGCAGTGGATGAAGGAGCAGAAGCGGATGAATGAGGCGGTG CAGGCACTGCGGCGCGCCCAGCTGCAGTATGTGCAACGCAGCGAGGACCTGCGGGCACGCTCCCAGGGGT CCCCTGAGGACTCGGCCCCCCAGGCCTCGCCGGGACCTAGCAAGCAGCAGGAGCGGCGGCGGCGCTCGCG AGAGGAGGCCCAGGCCAAGGCGCAGGAGGCCGAGGCGCTGTACCAGGCCTGTGTCCGCGAGGCCAACGCG CGGCAGCAGGACCTGGAGATCGCCAAGCAGCGAATCGTGTCGCACGTGCGCAAGCTGGTGTTTCAGGGGG ATGAAGTGCTGAGGCGGGTGACGCTGAGTCTCTTCGGGCTGCGGGGGGCGCAGGCAGAGCGTGGCCCCCG CGCCTTCGCCGCCCTGGCCGAGTGCTGTGCGCCCTTTGAGCCGGGCCAGCGCTACCAGGAGTTTGTACGG GCGCTGCGGCCCGAGGCCCCGCCGCCCCCGCCGCCCGCCTTCTCCTTCCAGGAGTTCCTTCCCTCCTTGA ACAGCTCCCCTCTGGACATCAGAAAGAAGCTCTCTGGGCCTCTTCCTCCAAGGCTGGATGAGAATTCAGC TGAGCCAGGCCCTTGGGAGGATCCGGGCACAGGCTGGCGCTGGCAAGGGACTCCAGGCCCCACTCCGGGC AGCGATGTGGACAGCGTGGGTGGCGGCAGCGAGTCTCGGTCCCTGGACTCACCCACTTCCAGCCCAGACC TGGGAGACGGGCTGGAGAATGGGCTGGGCAGCCCCTTCGGGAAGTGGACACTGTCCAGCGCGGCTCAGAC CCACCAGCTGCGGCGACTGCGGGGCCCAGCCAAGTGCCGCGAGTGCGAAGCCTTCATGGTCAGCGGGACG GAGTGTGAGGAGTGCTTTCTGACCTGCCACAAGCGCTGCCTGGAGACTCTCCTGATCCTCTGTGGACACA GGCGGCTCCCAGCCCGGACACCCCTTTTTGGGGTTGACTTCCTGCAGCTACCCAGGGACTTCCCGGAGGA GGTACCCTTTGTGGTCACGAAGTGCACGGCTGAGATAGAACACCGTGCCCTGGATGTGCAGGGCATTTAC CGGGTCAGCGGGTCCCGGGTCCGTGTGGAGCGGCTGTGCCAGGCTTTCGAGAATGGCCGAGCGTTGGTGG AGCTGTCGGGGAACTCGCCTCATGACGTCTCGAGTGTCCTCAAGCGATTTCTTCAGGAGCTCACCGAGCC CGTGATCCCCTTCCACCTCTACGACGCCTTCATCTCTCTGGCTAAGACCTTGCATGCAGACCCTGGGGAC GACCCTGGGACCCCCAGCCCCAGCCCTGAGGTTATCCGCTCGCTGAAGACCCTCTTGGTACAGCTGCCTG ACTCTAACTACAACACCCTGCGGCACCTGGTGGCCCATCTGTTCAGGGTGGCTGCACGATTTATGGAAAA CAAGATGTCTGCCAACAACCTGGGCATTGTGTTTGGGCCGACACTGCTGCGGCCGCCGGACGGCCCGCGG GCAGCCAGCGCCATCCCTGTCACCTGCCTGCTGGACTCTGGGCATCAGGCCCAGCTTGTGGAGTTCCTCA TCGTGCACTACGAGCAGATCTTTGGGATGGATGAGCTCCCCCAGGCCACTGAGCCCCCGCCCCAAGACTC CAGCCCAGCCCCTGGGCCCCTCACAACCAGCTCCCAACCGCCACCCCCGCACCTTGACCCAGACTCCCAG CCCCCAGTCCTAGCCTCAGACCCCGGCCCAGACCCCCAGCACCACAGTACCCTGGAGCAGCATCCCACGG CCACACCTACCGAGATTCCAACTCCACAGAGTGACCAGAGAGAGGACGTGGCTGAAGACACCAAAGATGG GGGAGGGGAAGTGTCCAGCCAAGGCCCAGAGGACTCACTCCTGGGGACACAGTCTCGTGGCCACTTCAGC CGCCAGCCAGTGAAGTATCCCCGGGGCGGTGTGAGGCCTGTAACCCACCAGCTGTCCAGTCTGGCCCTGG TGGCTTCCAAGCTGTGCGAGGAGACCCCCATCACATCAGTGCCCAGAGGGAGTTTGCGGGGGCGGGGGCC CAGCCCTGCAGCTGCCTCCCCTGAGGGCAGCCCCCTGCGCCGCACCCCGCTGCCCAAGCATTTTGAGATT ACCCAGGAGACAGCCCGGCTACTCTCGAAATTGGACAGCGAGGCTGTGCCCAGGGCCACCTGCTGCCCGG ACGTCCAGCCTGAGGAAGCCGAGGACCATCTCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001288999
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001288999.1, NP_001275928.1
RefSeq Size	3481 bp
RefSeq ORF	2835 bp
Locus ID	51291
UniProt ID	Q9P107
Protein Families	Druggable Genome
Gene Summary	This gene encodes a member of the ARHGAP family of Rho/Rac/Cdc42-like GTPase activating proteins. The encoded protein interacts with the Ras-related protein Gem through its N-terminal domain. Separately, it interacts with RhoA through a RhoGAP domain, and stimulates RhoA-dependent GTPase activity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] Transcript Variant: This variant (3) lacks an in-frame exon in the central coding region, compared to variant 1. The encoded isoform (3) is shorter, compared to isoform 1.

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