TTC7A (NM_001288951) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GIDID; MINAT; TTC7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001288951, the custom clone sequence may differ by one or more nucleotides ATGGCTGCGAAGGGCGCGCACGGCTCCTACCTGAAGGTGGAGAGCGAGCTGGAGCGCTGCCGCGCCGAGG GCCACTGGGACCGCATGCCGGAGCTGGTCCGGCAGCTGCAGACGCTGAGCATGCCCGGCGGCGGAGGTAA CAGGCGAGGCAGCCCGAGCGCAGCGTTCACCTTTCCGGACACCGATGACTTTGGGAAATTGCTGCTGGCT GAGGCCCTCCTGGAGCAGTGTTTGAAGGAGAACCATGCCAAAATAAAAGACTCCATGCCTTTGCTGGAGA AGAATGAGCCGAAGATGAGCGAAGCCAAAAATTATCTAAGCAGTATCCTTAACCATGGGAGGCTCTCGCC ACAGTACATGTGTGAGGCCATGCTGATCCTGGGCAAACTGCATTACGTGGAGGGCTCATACCGAGATGCC ATCAGCATGTACGCACGGGCCGGGATTGATGACATGTCCATGGAGAACAAGCCCCTGTATCAGATGCGGC TGCTGTCGGAGGCTTTTGTCATCAAAGGCCTCTCTCTGGAACGCCTACCCAACTCCATCGCCTCCCGCTT CCGCCTGACAGAGAGGGAGGAGGAAGTGATCACCTGTTTTGAGAGGGCCTCCTGGATCGCTCAGGTGTTC CTGCAGGAATTGGAGAAGACCACAAATAACAGCACGTCGAGGCATCTGAAAGGCTGTCACCCGCTTGACT ATGAGCTCACCTACTTCCTGGAAGCTGCCCTCCAGAGCGCCTATGTGAAAAACCTGAAGAAGGGGAACAT CGTGAAGGGCATGAGAGAGCTCCGGGAGGTGCTGCGGACTGTGGAGACCAAAGCAACTCAGAACTTCAAA GTGATGGCGGCCAAGCACCTGGCGGGGGTCCTGCTGCACTCCCTGAGTGAGGAGTGCTACTGGAGCCCCC TGTCCCACCCTCTGCCTGAGTTCATGGGCAAGGAGGAGAGTTCTTTCGCCACTCAGGCCCTGCGGAAACC TCACCTCTATGAAGGAGACAACCTCTACTGCCCCAAGGACAACATCGAGGAAGCCCTCCTGCTCCTCCTC ATCAGCGAATCCATGGCAACTCGAGATGTGGTGCTGAGCCGGGTGCCGGAGCAGGAGGAGGACCGGACAG TGAGCTTGCAGAATGCCGCAGCCATCTATGACCTCCTGAGCATCACGTTGGGCAGAAGGGGACAGTACGT CATGCTCTCGGAGTGCCTGGAGCGAGCCATGAAGTTTGCGTTTGGAGAATTTCACCTTTGGTACCAGGTG GCCCTCTCCATGGTGGCTTGTGGGAAGTCAGCCTACGCTGTGTCCCTGCTGCGGGAGTGTGTGAAGTTGC GGCCCTCGGACCCCACCGTGCCCCTGATGGCCGCGAAGGTCTGCATCGGGTCCCTTCGCTGGCTAGAGGA AGCAGAGCACTTTGCCATGATGGTGATCAGCCTCGGAGAGGAAGCCGGGGAGTTCCTCCCCAAGGGCTAC CTGGCTCTGGGTCTCACCTATAGCCTGCAGGCCACCGACGCCACCCTGAAGTCCAAGCAAGATGAATTGC ACCGGAAGGCACTGCAGACGCTGGAGAGGGCTCAGCAGCTGGCGCCCAGTGACCCCCAGGTCATCCTCTA TGTCTCGCTGCAGCTGGCCCTCGTCCGACAGATCTCCAGTGCCATGGAGCAGCTGCAGGAGGCCCTGAAG GTACGCAAGGATGATGCCCACGCCCTCCACCTGCTGGCACTGCTCTTCTCTGCCCAGAAGCACCACCAGC ATGCCCTGGATGTTGTCAACATGGCCATCACCGAGCACCCTGAGAACTTCAACCTGATGTTCACCAAGGT GAAGCTGGAGCAGGTGCTGAAAGGCCCAGAGGAAGCCCTCGTGACCTGCAGACAAGTGCTGAGGCTGTGG CAGACCCTGTACAGCTTCTCCCAGCTGGGAGACTTTAGGAGCCCAGAAGGCTTCCAGACTCCCCAGAGGA ACATCTGTAACAGTGAAATTTACAGAGGAGGAGGCCTAGAAAAGGATGGCAGCTTCGGTGAGGGCCTCAC CATGAAGAAGCAGAGTGGCATGCACCTGACTTTGCCTGATGCCCATGATGCAGACTCTGGCTCCCGGCGG GCTTCGTCCATCGCCGCCTCCCGGCTGGAGGAGGCCATGTCAGAGCTGACTATGCCCTCTTCGGTCCTGA AGCAGGGCCCCATGCAGCTGTGGACCACGCTGGAACAGATCTGGCTGCAGGCTGCTGAGCTGTTCATGGA GCAGCAGCACCTCAAGGAAGCAGGTTTCTGCATCCAGGAGGCGGCGGGCCTCTTCCCCACTTCTCACTCA GTACTCTATATGCGGGGCCGGCTGGCTGAGGTGAAGGGCAACCTGGAGGAGGCCAAGCAGCTGTACAAGG AGGCGCTCACGGTGAACCCAGATGGCGTGCGCATCATGCATAGCCTGGGTCTGATGCTGAGTCGGCTGGG CCACAAGAGCTTGGCCCAGAAGGTGCTTCGTGATGCCGTGGAGAGGCAGAGTACGTGCCACGAGGCGTGG CAGGGCCTGGGCGAGGTGCTGCAGGCCCAGGGCCAGAACGAGGCTGCCGTTGACTGCTTCCTCACCGCCC TTGAGCTGGAGGCCAGCAGCCCTGTACTGCCCTTCTCCATCATCCCCAGAGAGCTCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001288951
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001288951.1, NP_001275880.1
RefSeq Size	5247 bp
RefSeq ORF	2649 bp
Locus ID	57217
UniProt ID	Q9ULT0
Gene Summary	This gene encodes a protein containing tetratricopeptide repeats. Mutations in this gene disrupt intestinal development and can cause early onset inflammatory bowel disease and intestinal atresia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

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