MAP3K9 (NM_001284231) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MEKK9; MLK1; PRKE1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001284231, the custom clone sequence may differ by one or more nucleotides ATGGAGCTTACAGGACTGGAAGTTGCCCTGGTATTGATCCTCCAGAAGGTGGAGAATGGAGACCTGAGCA ACAAGATTCTGAAGATCACTGATTTTGGCCTGGCTCGGGAATGGCACCGAACCACCAAGATGAGTGCGGC AGGGACGTATGCTTGGATGGCACCCGAAGTCATCCGGGCCTCCATGTTTTCCAAAGGCAGTGATGTGTGG AGCTATGGGGTGCTACTTTGGGAGTTGCTGACTGGTGAGGTGCCCTTTCGAGGCATTGATGGCTTAGCAG TCGCTTATGGAGTGGCCATGAACAAACTCGCCCTTCCTATTCCTTCTACGTGCCCAGAACCTTTTGCCAA ACTCATGGAAGACTGCTGGAATCCTGATCCCCACTCACGACCATCTTTCACGAATATCCTGGACCAGCTA ACCACCATAGAGGAGTCTGGTTTCTTTGAAATGCCCAAGGACTCCTTCCACTGCCTGCAGGACAACTGGA AACACGAGATTCAGGAGATGTTTGACCAACTCAGGGCCAAAGAAAAGGAACTTCGCACCTGGGAGGAGGA GCTGACGCGGGCTGCACTGCAGCAGAAGAACCAGGAGGAACTGCTGCGGCGTCGGGAGCAGGAGCTGGCC GAGCGGGAGATTGACATCCTGGAACGGGAGCTCAACATCATCATCCACCAGCTGTGCCAGGAGAAGCCCC GGGTGAAGAAACGCAAGGGCAAGTTCAGGAAGAGCCGGCTGAAGCTCAAGGATGGCAACCGCATCAGCCT CCCTTCTGATTTCCAGCACAAGTTCACGGTGCAGGCCTCCCCTACCATGGATAAAAGGAAGAGTCTTATC AACAGCCGCTCCAGTCCTCCTGCAAGCCCCACCATCATTCCTCGCCTTCGAGCCATCCAGTGTGAGACTG TTTCCCAAATTAGCTGGGGCCAGAACACACAGGGGCACCTGTCCCCTGCTCTGTCCAGCCACCGACTGGT CCAGGCTTGCTCCATCCATAACTTCTGCCACCTCTCCTCGACAATGTGTATATACATGCACATATTGACA CCAGGTGAAAGCAGCAAAACCTGGGGCAGGAGCTCAGTCGTCCCAAAGGAGGAAGGGGAGGAGGAGGAGA AGAGGGCCCCAAAGAAGAAGGGACGGACGTGGGGGCCAGGGACGCTTGGTCAGAAGGAGCTTGCCTCGGG AGATGAAGGCCTCAAGTCCCTGGTAGATGGATATAAGCAGTGGTCGTCCAGTGCCCCCAACCTGGTGAAG GGCCCAAGGAGTAGCCCGGCCCTGCCAGGGTTCACCAGCCTTATGGAGATGGCCTTGCTGGCAGCCAGTT GGGTGGTGCCCATCGACATTGAAGAGGATGAGGACAGTGAAGGCCCAGGGAGTGGAGAGAGTCGCCTACA GCATTCACCCAGCCAGTCCTACCTCTGTATCCCATTCCCTCGTGGAGAGGATGGCGATGGCCCCTCCAGT GATGGAATCCATGAGGAGCCCACCCCAGTCAACTCGGCCACGAGTACCCCTCAGCTGACGCCAACCAACA GCCTCAAGCGGGGCGGTGCCCACCACCGCCGCTGCGAGGTGGCTCTGCTCGGCTGTGGGGCTGTTCTGGC AGCCACAGGCCTAGGGTTTGACTTGCTGGAAGCTGGCAAGTGCCAGCTGCTTCCCCTGGAGGAGCCTGAG CCACCAGCCCGGGAGGAGAAGAAAAGACGGGAGGGTCTTTTTCAGAGGTCCAGCCGTCCTCGTCGGAGCA CCAGCCCCCCATCCCGAAAGCTTTTCAAGAAGGAGGAGCCCATGCTGTTGCTAGGAGACCCCTCTGCCTC CCTGACGCTGCTCTCCCTCTCCTCCATCTCCGAGTGCAACTCCACACGCTCCCTGCTGCGCTCCGACAGC GATGAAATTGTCGTGTATGAGATGCCAGTCAGCCCAGTCGAGGCCCCTCCCCTGAGTCCATGTACCCACA ACCCCCTGGTCAATGTCCGAGTAGAGCGCTTCAAACGAGATCCTAACCAATCTCTGACTCCCACCCATGT CACCCTCACCACCCCCTCGCAGCCCAGCAGTCACCGGCGGACTCCTTCTGATGGGGCCCTTAAGCCAGAG ACTCTCCTAGCCAGCAGGAGCCCCTCCAGCAATGGGTTGAGCCCCAGTCCTGGAGCAGGAATGTTGAAAA CCCCCAGTCCCAGCCGAGACCCAGGTGAATTCCCCCGTCTCCCTGACCCCAATGTGGTCTTCCCCCCAAC CCCAAGGCGCTGGAACACTCAGCAGGACTCTACCTTGGAGAGACCCAAGACTCTGGAGTTTCTGCCTCGG CCGCGTCCTTCTGCCAACCGGCAACGGCTGGACCCTTGGTGGTTTGTGTCCCCCAGCCATGCCCGCAGCA CCTCCCCAGCCAACAGCTCCAGCACAGAGACGCCCAGCAACCTGGACTCCTGCTTTGCTAGCAGTAGCAG CACTGTAGAGGAGCGGCCTGGACTTCCAGCCCTGCTCCCGTTCCAGGCAGGGCCGCTGCCCCCGACTGAG CGGACGCTCCTGGACCTGGATGCAGAGGGGCAGAGTCAGGACAGCACCGTGCCGCTGTGCAGAGCGGAAC TGAACACACACAGGCCTGCCCCTTATGAGATCCAGCAGGAGTTCTGGTCTTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001284231
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001284231.1, NP_001271160.1
RefSeq Size	10742 bp
RefSeq ORF	2643 bp
Locus ID	4293
Protein Families	Druggable Genome, Protein Kinase
Gene Summary	Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade through the phosphorylation of MAP2K4/MKK4 and MAP2K7/MKK7 which in turn activate the JNKs. The MKK/JNK signaling pathway regulates stress response via activator protein-1 (JUN) and GATA4 transcription factors. Plays also a role in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR and contains multiple differences in the coding region, compared to variant 1. It initiates translation at an alternate start codon. The encoded isoform (3) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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