RPS6KC1 (NM_001287219) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	humS6PKh1; RPK118; RSKL1; S6K-delta-1; S6PKh1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001287219, the custom clone sequence may differ by one or more nucleotides ATGGCTTCCAATCAAAATTCTCCCATTAGAACTTTTGGTCTCAATCTTTCTTCGGATTCTTCAGCACTAG GGGCTGTTGCTTCTGACAGTGAACAGAGCAAAACAGAAGAAGAACGGGAAAGTCGTAGCCTCTTTCCTGG CAGTTTAAAGCCGAAGCTTGGCAAGAGAGATTATTTGGAGAAAGCAGGAGAATTAATAAAGCTGGCTTTA AAAAAGGAAGAAGAAGACGACTATGAAGCTGCTTCTGATTTTTATAGGAAGGGAGTTGATTTACTCCTAG AAGGTGTTCAAGGAGAGTCAAGCCCTACCCGTCGAGAAGCTGTGAAGAGAAGAACAGCCGAGTACCTCAT GCGGGCAGAAAGTATCTCTAGTCTTTATGGGAAACCTCAGCTTGATGATGTATCTCAGGTTTTACTTGTA ATGGACACAAGGACAGAACAGACTTTCATTTTAAAAGGTCTAAGGAAAAGCAGTGAATACAGCAGGAACA GAAAGACCATCATCCCCCGCTGTGTGCCCAACATGGTGTGTCTGCATAAGTACATCATCTCTGAGGAGTC AGTATTTCTTGTGCTGCAGCATGCGGAAGGTGGCAAACTGTGGTCATATATCAGTAAATTTCTAAACAGA AGTCCTGAAGAAAGCTTTGACATCAAGGAAGTGAAAAAACCTACACTTGCAAAAGTTCACCTGCAGCAGC CAACTTCTAGTCCTCAGGACAGCAGTAGCTTTGAATCCAGAGGAAGTGATGGTGGAAGCATGCTTAAAGC TCTGCCTTTGAAGAGTAGTCTTACTCCAAGTTCTCAAGATGACAGCAACCAGGAAGATGATGGCCAAGAT AGCTCTCCAAAGTGGCCAGATTCTGGTTCAAGTTCAGAAGAAGAATGTACTACTAGTTATTTAACATTAT GCAATGAATATGGGCAAGAAAAGATTGAACCAGGGTCTTTGAATGAGGAGCCCTTCATGAAGACTGAAGG GAATGGTGTTGATACAAAAGCTATTAAAAGCTTCCCAGCACACCTTGCTGCTGACAGTGACAGCCCCAGC ACACAGCTGAGAGCTCACGAGCTGAAGTTCTTCCCCAACGATGACCCAGAAGCAGTTAGTTCTCCAAGAA CATCAGATTCCCTCAGTAGATCAAAAAATAGCCCCATGGAATTCTTTAGGATAGACAGTAAGGATAGCGC AAGTGAACTCCTGGGACTTGACTTTGGAGAAAAATTGTATAGTCTAAAATCAGAACCTTTGAAACCATTC TTTACTCTTCCAGATGGAGACAGTGCTTCTAGGAGTTTTAATACTAGTGAAAGCAAGGTAGAGTTTAAAG CTCAGGACACCATTAGCAGGGGCTCAGATGACTCAGTGCCAGTTATTTCATTTAAAGATGCTGCTTTTGA TGATGTCAGTGGTACTGATGAAGGAAGACCTGATCTTCTTGTAAATTTACCTGGTGAATTGGAGTCAACA AGAGAAGCTGCAGCAATGGGACCTACTAAGTTTACACAAACTAATATAGGGATAATAGAAAATAAACTCT TGGAAGCCCCTGATGTTTTATGCCTCAGGCTTAGTACTGAACAATGCCAAGCACATGAGGAGAAAGGCAT AGAGGAACTGAGTGATCCCTCTGGGCCCAAATCCTATAGTATAACAGAGAAACACTATGCACAGGAGGAT CCCAGGATGTTATTTGTAGCAGCTGTTGATCATAGTAGTTCAGGAGATATGTCTTTGTTACCCAGCTCAG ATCCTAAGTTTCAAGGACTTGGAGTGGTTGAGTCAGCAGTAACTGCAAACAACACAGAAGAAAGCTTATT CCGTATTTGTAGTCCACTCTCAGGTGCTAATGAATATATTGCAAGCACAGACACTTTAAAAACAGAAGAA GTATTGCTGTTTACAGATCAGACTGATGATTTGGCTAAAGAGGAACCAACTTCTTTATTCCAGAGAGACT CTGAGACTAAGGGTGAAAGTGGTTTAGTGCTAGAAGGAGACAAGGAAATACATCAGATTTTTGAGGACCT TGATAAAAAATTAGCACTAGCCTCCAGGTTTTACATCCCAGAGGGCTGCATTCAAAGATGGGCAGCTGAA ATGGTGGTAGCCCTTGATGCTTTACATAGAGAGGGAATTGTGTGCCGCGATTTGAACCCAAACAACATCT TATTGAATGATAGAGGACACATTCAGCTAACGTATTTTAGCAGGTGGAGTGAGGTTGAAGATTCCTGTGA CAGCGATGCCATAGAGAGAATGTACTGTGCCCCAGAGGTTGGAGCAATCACTGAAGAAACTGAAGCCTGT GATTGGTGGAGTTTGGGTGCTGTCCTCTTTGAACTTCTCACTGGCAAGACTCTGGTTGAATGCCATCCAG CAGGAATAAATACTCACACTACTTTGAACATGCCAGAATGTGTCTCTGAAGAGGCTCGCTCACTCATTCA ACAGCTCTTGCAGTTCAATCCTCTGGAACGACTTGGTGCTGGAGTTGCTGGTGTTGAAGATATCAAATCT CATCCATTTTTTACCCCTGTGGATTGGGCAGAACTGATGAGATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001287219
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001287219.1, NP_001274148.1
RefSeq Size	4243 bp
RefSeq ORF	2565 bp
Locus ID	26750
Protein Families	Druggable Genome, Protein Kinase
Gene Summary	Sphingosine kinase catalyzes the formation of sphingosine 1 phosphate, a lipid cellular messenger. The protein encoded by this gene can bind to sphingosine kinase and to phosphatidylinositol 3-phosphate, suggesting a role in sphingosine 1 phophate signaling. The encoded protein can also bind to peroxiredoxin-3 and may help transport it to mitochondria. [provided by RefSeq, Mar 2017] Transcript Variant: This variant (4) differs in its 5' UTR, initiates translation at a downstream start codon, and lacks an in-frame exon in the central coding region, compared to variant 1. The encoded isoform (c) is shorter, compared to isoform a. Variants 3, 4, and 15 all encode the same isoform (c). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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