DNAI3 (NM_001288563) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DIC3; NYD-SP29; WDR63
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001288563, the custom clone sequence may differ by one or more nucleotides ATGGCTCCAAAACAAAAGAAAAAGACATCACGTGGCAAAAAAAGACTAAAACCAGTATTAGCTGCTAGTG AAGACATGGAACCAGTAAATATGGAGAGCATGGGTCATCCAGAAATTTATCCTTTAGTATTAACCACCAA GACCCAAGAAATATTTAACTGCCGAATAGATGAAGATGTCACAGATGAACAACCTTATAAGCTTATCAAT AAAGAAGACATTTTTGAGGACCTGCGCAACAGAGCTGCAGTATCTGATTTCCACCCAGTCAAAAAAATTG TCCAGGAATATCCTGGAAATGAGCTTCTGCTTGTTTATGACAAAGACTTCAAATATGGACTTAACTTTTA TCTTATTGCAACTGAAGAGGGCAAAGAAAACTATTTAAATCCCCCAGAAGTACCAGAAGAACAAGAAGAA TATAAAGAACATATTCCTGAAGATGTGTATATTTATAAACCACCTGTCTCTAAACCATGGGTTTCTTTGG GCAGTGAAAAAGAAATTGAGGAAGAATCAGTTACGGAATCTACAAAGCAGATTACATATATGATTTCTCG AAAACGAAGTGAATTTGGTGCACCAATTAAGTTCAGTGACCAGAATGCTTCCAGTGTAAAAGATGCCTAT ATTGAATGTACAGCCTACCCAGATAAAAATTTTACCCTTAAACAACTTGAAAAAGATGTTGGCATGCAAG TAATCCCCCAAATAAAGGACATAAGCACTCAGACAAAATGTGTTGAAATAGCCCTGCAGCAAAATGAAAT CATGAACACATTTATTGATGACTGGAAATACCTCGCAGAAGAAGAAGGCACCTTTGGGGACAAGACCGAT ACCCACCTGAAAGAGTACCAGTCCTTTACCGACCTTCATAGCCCAACGGAGAAAATGATTACCTGTGTCT CATGGCATCCAACTATCTATGGGCTAATAGCTGTGTCGGTAGCCGTGCGACTTTCTTTTGAAGACAGAGT TCACTTTTCTGGTAAATTATTGCTGCAGCCATCACTGATTCTTTTCTGGAGCTTCTCTGATCCTATACAT CCTCAGTTAATGCTGGAGAGCCCAGATGACATCTTCTGCTTCAAGTTCTGTCCGAGTGATCCTAATATCA TTGCTGGAGGCTGTATCAATGGGCAGATTGTCATGTGGGATATCACCGCACATGCAGATCGCATAGAAAA CATTAAGGCAGGTGGTAGTAGAAGTAAAAGAGCCACACTGAAGCCTATGTTTCTCCTTGAACCGGAGAGT AATAAAGAAGCAATGTATATCAGACACTGTGCAGTCTCTTCAATAGAAAATGGACATAAGAAAGTAATTA CAGATATACACTGGTTGTCTGACACATTTGAGATTAACAGAATGGGCTCCGTCTTTGAGAATCGAAGTGG AATATGCTGTCAACTTGTCACATGTTCAGCAGATTGCACAATATGTTTTTGGGATATTAGACCACAGAAA CCTTTAACCCCCCAAACAACAGAGAAAAAGAAGGAGGAAAGTATTGAAATTCCTTTTGATGTACCATCTA CTTTTTTGCATCTGGATCTCTCCTGGAAACCTCTCACTAAGGTAAGGCTGTCCAAGGGTGAAACAAGTTT AGACCACTGTCCAACCAAGATAAGCCTGAATGAAGACCATCTTCTTTGCAAAACACAAGACAAAATGTTA GCACAGAGCAAAACAGAGAAGGCAGAAGAAATGAACCCGTATCATAATCTGGAAAGTGGGATGGCCAATC TTCTCAAGCCAATAGATGACTTCTGCACAAAGTTCTTTGTGGGAACAGAGGAAGGCGAAGTGATATACAC AGATTGGAAAATGGAAAAAGACCCTGAAACTGGCCGACTTATGTCAAAGAAGCCAGTGAGCCACCACACC ATTCACGACGGAACTGTCCACACTATTCAGAGATCACCTTTCTACAACGACATTATTCTCACGGTTGGAG GTTGGAACGTGGCCATATGGAAAGAAGGTGTTATGACTGGACCGCTCCTTCAGTCATGCTGTGCACCAAA AAGGTACACCTCAGGCCACTGGTCCCTGACTCGGCCCGGAGTTTTCTACATCGGCCGAGAAGATGGATAC ATTGATATCTGGGACCTTCTGGAGAAAACCCATGAACCAGCCCAGTCTCAAAACATTTGCATAACTATGA TCACCTACATCAAACCCTGGATCTTTTCTTCTAAACAGCAATTTATAGCCACAGCTGATTATTATGGAAC ACTGCATATATTAGAAATTCCTTGGACATTAAGTCGCCCTTCCACCAATGAGATGGCAAGTGTCAACCAC TATTTTGAAAGAGAAGTCAAGCATCTGGAATACGTAGAACAGCGCAAAAAAATTCGTGAGCAAGAAAAGA AAGAAATGGAACTAGAAATGGCAAAGAAAAAAGTTAAAACATATCAGAAGTCAAAAGAACAAATGCAGGC TGAATTAAAAATGGACTATGAGAGTTATCTGGAACTGGAAAAGACTGTTCTTATCAACCTTGGCCTAATC AAAGTCACAGAGAAGGGGTCATACATGGAGGTGATGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001288563
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001288563.1, NP_001275492.1
RefSeq Size	2921 bp
RefSeq ORF	2559 bp
Locus ID	126820
UniProt ID	Q8IWG1
Gene Summary	Acts as a negative regulator of cell migration, invasion, and metastasis downstream of p53/TP53, through inhibition of Arp2/3 complex-mediated actin polymerization (PubMed:32128961). Via its association with the multisubunit axonemal dynein complex, is potentially involved in the regulation of cilia function (By similarity). May play a role in osteogenesis of dental tissue-derived mesenchymal stem cells (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an in-frame exon in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.

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