GFM2 (NM_001281302) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	EF-G2mt; EFG2; hEFG2; mEF-G 2; MRRF2; MST027; MSTP027; RRF; RRF2; RRF2mt
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001281302, the custom clone sequence may differ by one or more nucleotides ATGAAAAGGGAATGGATTCAAGGCCAGGCATGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCG AGACAGTAACAGTTTGGAGGATCAAGATGTTGACCAACTTGAGGATATTTGCAATGAGTCATCAGACAAT ACCCAGTGTGTATATTAATAATATATGCTGCTATAAAATAAGAGCAAGTTTAAAAAGATTAAAGCCACAT GTGCCGCTTGGAAGAAATTGCAGTTCTCTACCAGGCTTAATAGGAAATGATATCAAATCCCTTCATTCCA TCATCAATCCTCCCATAGCTAAAATCCGTAATATTGGAATTATGGCTCATATTGATGCAGGCAAAACTAC CACCACAGAAAGAATATTGTACTATTCCGGATATACAAGATCACTGGGAGATGTTGATGATGGAGACACA GTGACAGATTTCATGGCCCAAGAGCGAGAAAGAGGCATTACTATTCAATCAGCTGCTGTTACATTTGATT GGAAAGGTTATAGAGTCAATCTAATTGATACACCAGGTCATGTGGACTTTACCTTGGAGGTTGAGCGGTG CCTAAGAGTGTTGGATGGTGCAGTGGCTGTATTTGATGCCTCTGCTGGTGTAGAGGCCCAGACTCTCACA GTATGGAGGCAAGCTGATAAACACAATATACCTCGAATCTGTTTTTTAAACAAGATGGACAAAACTGGAG CAAGCTTTAAGTATGCAGTTGAAAGCATCAGAGAGAAGTTAAAGGCAAAGCCTTTGCTTTTACAGTTACC AATTGGTGAAGCCAAAACTTTCAAAGGAGTGGTGGATGTAGTAATGAAAGAAAAACTTCTTTGGAATTGC AATTCAAATGATGGAAAAGACTTTGAGAGAAAGCCCCTCTTGGAAATGAATGATCCTGAATTGCTGAAGG AAACAACTGAAGCAAGGAATGCCTTAATTGAACAAGTTGCAGATTTGGATGATGAATTTGCTGACTTGGT TTTAGAAGAATTTAGTGAGAATTTTGATTTGTTACCAGCTGAAAAGCTACAGACTGCAATACATAGAGTG ACACTAGCTCAGACAGCAGTGCCTGTGCTTTGTGGAAGTGCCCTGAAAAACAAAGGGATACAGCCCTTGT TAGATGCTGTTACTATGTACTTACCTTCACCTGAAGAGCGTAACTATGAATTTCTGCAGTGGTATAAGGA TGACTTATGTGCATTGGCATTTAAAGTTCTCCATGACAAGCAGCGAGGACCACTGGTTTTTATGCGCATT TACTCAGGCACTATAAAACCCCAGTTGGCCATTCATAATATTAATGGAAACTGCACGGAGAGAATAAGTC GTCTGCTTTTGCCGTTTGCTGACCAACATGTAGAAATCCCTTCATTGACTGCTGGTAACATTGCTTTGAC TGTTGGGCTTAAACATACTGCCACTGGAGACACCATTGTCTCATCCAAGTCCAGTGCATTAGCTGCAGCT CGTAGAGCCGAACGGGAGGGAGAAAAGAAGCACAGACAAAACAATGAAGCAGAGAGACTTTTATTGGCTG GAGTGGAGATTCCAGAACCTGTTTTCTTCTGTACCATAGAACCCCCATCACTGTCTAAGCAGCCAGATTT GGAACATGCGTTGAAATGTCTTCAGCGTGAAGATCCCAGTTTGAAAGTGAGGCTAGATCCTGACTCTGGA CAAACTGTTCTGTGTGGTATGGGGGAGTTACATATAGAGATTATTCATGATCGAATCAAGAGGGAATATG GACTGGAGACCTATCTCGGGCCTCTCCAGGTGGCATATCGAGAGACCATCCTAAACTCAGTTCGTGCCAC AGATACCTTAGATAGAACTTTAGGAGACAAAAGGCATCTTGTGACTGTAGAAGTGGAAGCAAGGCCAATT GAAACATCATCTGTTATGCCTGTGATTGAGTTTGAGTATGCTGAAAGTATCAATGAAGGCCTTTTGAAGG TCTCCCAAGAGGCCATTGAAAATGGAATTCACAGCGCATGTCTCCAAGGACCATTGCTTGGATCCCCAAT TCAGGATGTAGCAATTACTTTACATTCCCTGACAATTCATCCTGGCACCTCCACAACTATGATTTCTGCC TGTGTCTCAAGATGCGTGCAAAAGGCTCTGAAGAAAGCTGATAAGCAAGTTTTGGAGCCTCTGATGAATC TTGAGGTTACAGTAGCTAGAGATTATCTCAGCCCTGTCCTGGCAGATCTGGCACAAAGAAGAGGAAACAT TCAGGAAATTCAGACTCGCCAGGACAACAAAGTTGTTATTGGATTTGTTCCCTTAGCAGAAATTATGGGT TATTCAACTGTGCTTCGAACGCTAACATCAGGCTCAGCTACTTTTGCCTTAGAACTATCTACTTATCAAG CCATGAATCCTCAAGATCAAAATACACTGCTCAACCGGAGAAGTGGTTTGACCTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001281302
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001281302.1, NP_001268231.1
RefSeq Size	3382 bp
RefSeq ORF	2436 bp
Locus ID	84340
UniProt ID	Q969S9
Gene Summary	Eukaryotes contain two protein translational systems, one in the cytoplasm and one in the mitochondria. Mitochondrial translation is crucial for maintaining mitochondrial function and mutations in this system lead to a breakdown in the respiratory chain-oxidative phosphorylation system and to impaired maintenance of mitochondrial DNA. This gene encodes one of the mitochondrial translation elongation factors, which is a GTPase that plays a role at the termination of mitochondrial translation by mediating the disassembly of ribosomes from messenger RNA . Its role in the regulation of normal mitochondrial function and in disease states attributed to mitochondrial dysfunction is not known. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (4).

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