DRC7 (NM_001289163) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C16orf50; CCDC135; CFAP50; FAP50
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001289163, the custom clone sequence may differ by one or more nucleotides ATGGAGGTCCTGAGGGAGAAGGTGGAGGAGGAGGAGGAGGCCGAGCGGGAGGAGGCGGCCGAGTGGGCTG AATGGGCGAGGATGGAGAAAATGATGAGGCCAGTTGAGGTGCGGAAGGAGGAAATCACCTTAAAGCAGGA GACGCTCAGAGACCTGGAGAAGAAGCTGTCAGAGATCCAGATCACTGTCTCAGCGGAGCTCCCGGCCTTT ACCAAGGACACTATTGACATCTCCAAGCTGCCCATTTCCTACAAAACCAACACACCCAAGGAGGAACACC TGCTGCAGGTGGCAGACAACTTCTCCCGCCAGTACAGCCATCTGTGCCCGGACCGCGTGCCCCTCTTCCT GCACCCCCTGAACGAGTGTGAAGTGCCCAAGTTCGTGAGCACAACCCTCCGGCCCACACTGATGCCCTAC CCCGAGCTCTACAACTGGGACAGCTGTGCCCAGTTTGTCTCCGACTTCCTCACCATGGTGCCCCTGCCTG ACCCTCTCAAGCCGACCATCAAGAAGGAGGAAAAGGTGCTGCCTAAGAAGTATACCATCAAACCCCCCAG GGACCTGTGCAGCAGGTTTGAGCAGGAGCAAGAGGTGAAGAAGCAGCAGGAGATCAGAGCCCAGGAGAAG AAGCGGCTGAGGGAGGAGGAGGAGCGCCTCATGGAAGCGGAGAAGGCAAAGCCGGATGCCCTGCACGGCC TGCGGGTGCACTCCTGGGTCCTTGTGCTATCGGGGAAGCGCGAGGTGCCTGAGAACTTCTTCATCGACCC ATTCACAGGACATAGCTACAGCACCCAGGATGAGCACTTCCTGGGCATCGAAAGCCTGTGGAACCACAAG AACTACTGGATCAACATGCAGGATTGCTGGAACTGCTGCAAGGACTTGATCTTTGACCTGGGTGACCCTG TGAGATGGGAGTACATGCTCCTGGGGACTGATAAGTCTCAGCTGTCCTTGACTGAAGAAGACGACAGTGG GATAAACGATGAGGATGATGTGGAAAATCTGGGCAAGGAGGATGAGGATAAGAGCTTCGACATGCCCCAC TCGTGGGTGGAGCAGATTGAGATCTCCCCGGAAGCATTTGAGACCCGCTGCCCGAACGGGAAGAAGGTGA TTCAGTACAAGAGGGCAAAGCTGGAGAAGTGGGCCCCGTACCTCAATAGCAATGGCCTTGTGAGCCGCCT CACCACCTATGAGGACTTGCAGTGTACCAATATTTTGGAGATAAAGGAGTGGTACCAGAACCGGGAAGAC ATGCTGGAGCTGAAACACATAAACAAGACCACAGACCTGAAGACAGACTACTTCAAGCCTGGCCACCCCC AGGCTCTGCGCGTGCACTCGTACAAGTCCATGCAACCTGAGATGGACCGTGTCATTGAGTTTTATGAAAC GGCCCGTGTGGATGGCCTGATGAAGCGGGAGGAGACACCCAGGACAATGACAGAGTACTATCAAGGACGC CCAGACTTCCTCTCCTACCGCCATGCCAGCTTCGGACCCCGAGTCAAGAAGCTCACTCTGAGCAGTGCAG AGTCAAACCCCCGGCCCATTGTGAAAATCACAGAGCGGTTCTTCCGCAACCCAGCGAAGCCCGCGGAGGA GGACGTGGCAGAGCGCGTGTTTCTGGTCGCGGAGGAGCGCATCCAGCTGCGCTACCACTGCCGTGAGGAC CACATCACGGCCTCCAAGCGCGAGTTCCTGCGGCGCACCGAGGTGGACAGCAAAGGCAACAAGATCATCA TGACGCCCGACATGTGCATCAGCTTCGAGGTGGAGCCCATGGAGCACACCAAGAAGCTGCTCTACCAGTA CGAGGCCATGATGCACCTGAAGAGGGAGGAGAAGCTGTCCAGACATCAGGTCTGGGAGTCAGAGCTGGAG GTGCTGGAGATTCTGAAGCTTCGAGAGGAAGAGGAGGCGGCGCACACACTGACCATCTCCATCTATGACA CCAAGCGGAATGAGAAGAGCAAGGAATATCGGGAGGCCATGGAGCGCATGATGCACGAAGAGCACCTGCG GCAGGTGGAGACCCAGCTGGACTACCTGGCCCCATTCCTGGCCCAGCTCCCGCCAGGAGAGAAACTAACA TGCTGGCAGGCGGTGCGCCTCAAGGATGAGTGCCTCAGCGACTTCAAGCAGCGGCTCATCAACAAGGCCA ACCTCATCCAGGCCCGCTTTGAGAAGGAGACCCAGGAGCTGCAAAAGAAGCAGCAGTGGTACCAGGAGAA CCAGGTGACGCTGACACCCGAGGATGAAGACCTGTACCTGAGTTACTGCTCTCAGGCCATGTTCCGCATC CGCATCCTGGAGCAGCGCCTCAATCGACACAAGGAACTGGCCCCACTGAAGTACCTGGCTCTGGAGGAAA AGCTCTACAAGGACCCACGCCTGGGGGAGCTCCAGAAAATATTCGCTTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001289163
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289163.1, NP_001276092.1
RefSeq Size	2731 bp
RefSeq ORF	2430 bp
Locus ID	84229
UniProt ID	Q8IY82
Gene Summary	Component of the nexin-dynein regulatory complex (N-DRC) a key regulator of ciliary/flagellar motility which maintains the alignment and integrity of the distal axoneme and regulates microtubule sliding in motile axonemes. Involved in the regulation of flagellar motility.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR and lacks an in-frame exon in the central coding region, compared to variant 1. The encoded isoform (b) is shorter, compared to isoform a.

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