PHF15 (JADE2) (NM_001289984) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	JADE-2; PHF15
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001289984, the custom clone sequence may differ by one or more nucleotides ATGGAAGAGAAGAGGCGAAAATACTCCATCAGCAGTGACAACTCTGACACCACTGACAGTCATGCGACAT CTACATCCGCATCAAGATGCTCCAAACTGCCCAGCAGCACCAAGTCGGGCTGGCCCCGACAGAACGAAAA GAAGCCCTCCGAGGTTTTCCGGACAGACTTGATCACAGCCATGAAGATCCCGGACTCATACCAGCTCAGC CCGGATGACTACTACATCCTGGCAGACCCATGGCGACAGGAATGGGAGAAAGGTGTGCAGGTGCCTGCCG GGGCAGAGGCCATCCCAGAGCCCGTGGTGAGGATCCTCCCACCACTGGAAGGCCCCCCTGCCCAGGCATC CCCGAGCAGCACCATGCTTGGTGAGGGCTCCCAGCCTGATTGGCCAGGGGGCAGCCGCTATGACTTGGAC GAGATTGATGCCTACTGGCTGGAGCTCATCAACTCGGAGCTTAAGGAGATGGAGAGGCCGGAGCTGGACG AGCTGACATTAGAGCGTGTGCTGGAGGAGCTGGAGACCCTGTGCCACCAGAATATGGCCAGGGCCATTGA GACGCAGGAGGGGCTGGGCATCGAGTACGACGAGGATGTTGTCTGCGACGTGTGTCGCTCTCCTGAGGGC GAGGATGGCAACGAGATGGTCTTCTGTGACAAGTGCAACGTCTGTGTGCATCAGGCATGCTACGGGATCC TCAAGGTGCCCACGGGCAGCTGGCTGTGCCGGACGTGTGCCCTGGGTGTCCAGCCAAAGTGCCTGCTCTG CCCCAAGCGAGGAGGAGCCTTGAAGCCCACTAGAAGTGGGACCAAGTGGGTGCATGTCAGCTGTGCCCTA TGGATTCCTGAGGTCAGCATCGGCTGCCCAGAGAAGATGGAGCCCATCACCAAGATCTCGCATATCCCAG CCAGCCGCTGGGCTCTGTCCTGCAGCCTCTGCAAGGAATGCACAGGCACCTGCATCCAGTGTTCCATGCC TTCCTGCGTCACAGCGTTCCATGTCACATGCGCCTTTGACCACGGCCTGGAAATGCGGACTATATTAGCA GACAACGATGAGGTCAAGTTCAAGTCATTCTGCCAGGAGCACAGTGACGGGGGCCCACGTAATGAGCCCA CATCTGAGCCCACGGAACCCAGCCAGGCTGGCGAGGACCTGGAAAAGGTGACCCTGCGCAAGCAGCGGCT GCAGCAGCTAGAGGAGGACTTCTACGAGCTGGTGGAGCCGGCTGAGGTGGCTGAGCGGCTGGACCTGGCT GAGGCACTGGTCGACTTCATCTACCAGTACTGGAAGCTGAAGAGGAAAGCCAATGCCAACCAGCCGCTGC TGACCCCCAAGACCGACGAGGTGGACAACCTGGCCCAGCAGGAGCAGGACGTCCTCTACCGCCGCCTGAA GCTCTTCACCCATCTGCGGCAGGACCTAGAGAGGGTTAGAAATCTGTGCTACATGGTGACAAGGCGCGAG AGAACGAAACACGCCATCTGCAAACTCCAGGAGCAGATATTCCACCTGCAGATGAAACTTATTGAACAGG ATCTGTGTCGAGCAGGCCTGTCCACCTCATTCCCCATCGATGGCACCTTCTTCAACAGCTGGCTGGCACA GTCGGTGCAGATCACAGCAGAGAACATGGCCATGAGCGAGTGGCCACTGAACAATGGGCACCGCGAGGAC CCTGCTCCAGGGCTGCTGTCAGAGGAACTGCTGCAGGACGAGGAGACACTGCTCAGCTTCATGCGGGACC CCTCGCTGCGACCTGGTGACCCTGCTAGGAAGGCCCGAGGCCGCACCCGCCTGCCTGCCAAGAAGAAACC ACCACCACCACCACCGCAGGACGGGCCTGGTTCACGGACGACTCCAGACAAAGCCCCCAAGAAGACCTGG GGCCAGGATGCAGGCAGTGGCAAGGGGGGTCAAGGGCCACCTACCAGGAAGCCACCACGTCGGACATCTT CTCACTTGCCGTCCAGCCCTGCAGCCGGGGACTGTCCCATCCTAGCCACCCCTGAAAGCCCCCCGCCACT GGCCCCTGAGACCCCGGACGAGGCAGCCTCAGTAGCTGCTGACTCAGATGTCCAAGTGCCTGGCCCTGCA GCAAGCCCTAAGCCTTTGGGCCGGCTCCGGCCACCCCGCGAGAGCAAGGTAACCCGGAGATTGCCGGGTG CCAGGCCTGATGCTGGGATGGGACCACCTTCAGCTGTGGCTGAGAGGCCCAAGGTCAGCCTGCATTTTGA CACTGAGACTGATGGCTACTTCTCTGATGGGGAGATGAGCGACTCAGATGTAGAGGCCGAGGACGGTGGG GTGCAGCGGGGTCCCCGGGAGGCAGGGGCAGAGGAGGTGGTCCGCATGGGCGTACTGGCCTCCTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289984
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289984.1, NP_001276913.1
RefSeq Size	6664 bp
RefSeq ORF	2376 bp
Locus ID	23338
UniProt ID	Q9NQC1
Protein Families	Druggable Genome, Transcription Factors
Gene Summary	Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo (PubMed:16387653). Acts as a E3 ubiquitin-protein ligase mediating the ubiquitination and subsequent proteasomal degradation of target protein histone demethylase KDM1A (PubMed:25018020). Also acts as a ubiquitin ligase E3 toward itself. Positive regulator of neurogenesis (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1.

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