KIAA1586 (NM_001286274) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001286274, the custom clone sequence may differ by one or more nucleotides ATGGGAGACCCGGGGTCGGAAATAATAGAATCTGTCCCTCCAGCTGGCCCTGAGGCATCTGAGTCAACAA CGGATGAAAATGAAGACGACATTCAGTTTGTCAGTATTCTGTTTCCTAAAATGCCAAAACGACAGGGTGA TTTTTTGCATTTTTTAAATGTGAAGAAGGTGAAAACAGACACAGAAAATAATGAAGTGAGCAAAAATCAC TGCAGATTGTCTAAGGCAAAGGAACCACATTTCGAGTATATTGAACAACCAATCATTGAAGAAAAGCCAT CACTTTCATCAAAGAAAGAAATAGATAATCTTGTGCTTCCAGATTGTTGGAATGAAAAACAAGCATTTAT GTTTACAGAACAATACAAATGGCTTGAAATAAAAGAAGGTAAATTAGGATGTAAGGATTGTTCAGCAGTT CGGCATTTGGGATCGAAAGCAGAAAAGCATGTCCATGTGTCCAAGGAATGGATTGCATATTTAGTAACCC CTAATGGCAGTAATAAAACTACTAGGCAAGCTTCTCTACGAAAAAAAATTAGGGAACATGATGTTTCTAA AGCCCATGGTAAAATTCAGGATTTGTTAAAGGAATCAACTAATGATTCAATTTGTAATTTAGTGCATAAA CAAAATAATAAAAATATTGATGCTACTGTAAAAGTTTTCAATACTGTTTACAGTTTAGTAAAACATAACA GACCTTTATCTGATATTGAGGGGGCAAGAGAATTACAGGAAAAAAATGGAGAGGTAAATTGTTTAAATAC ACGTTACAGTGCAACAAGAATAGCAGAACATATTGCAAAAGAAATGAAGATGAAGATATTTAAGAATATT ATAGAAGAGAATGCCAAAATCTGTATCATAATTGATGAGGCATCTACAGTTTCAAAGAAAACCACCCTAG TGATTTATCTCCAGTGCACAATTCAGTCAGCTCCTGCACCTGTTATGTTATTTGTGGCTTTAAAAGAATT GGTGTCAACTATAGCAGAGTGTATTGTCAATACATTATTGACTACTTTAAATGATTGTGGTTTTACAAAT GAATATTTGAAAGCAAATTTAATTGCATTTTGTTCTGATGGTGCTAATACAATCCTGGGAAGAAAGTCTG GAGTAGCTACAAAATTGTTAGAAAATTTTCCTGAAATCATCATTTGGAACTGTTTAAATCATCGATTACA ATTGTCACTTGATGATTCTATATCCGAAATAAAACAAATTAATCATTTAAAAATATTTATTGATAAAATT TATTCTATTTATCATCAACCTAATAAAAATCAAACCAAGCTTCTAGGAACTGTAGCTAAAGAACTTGAAA CTGAAATTATTAAAATTGGTCGAGTAATGGGACCAAGATGGGCGGCATGTAGTTTACAAGCTGCTACTGC TGTATGGCATGCATATCCTATATTATATATGCATTTTTCTCATTCTTACTCTGGTTTGGCGAAGAGATTA GCTAACATTAATTTCTTACAAGACCTTGCTTTAATGATTGACATTCTTGAAGAATTTTCAGTACTTTCAA CTGCATTACAGTCAAGATCAACTAATATTAAGAAAGCACAAAAATTGATCAAACGTACCATAAGAGCTTT GGAAAATTTAAAAATTGGTACTGGAAAGTATGAATCTCAAATTGAAGATTTGATCAAGTCAGATAAGTTT AAAGATATTCCATTTAATAAAAACAATAAATTTAATGCTCTTCCTAGGAGTATATTACTAGACAATATAA TTCAGCACATGAACCTACGCCTTTTATCTGACAGAAACCATGAAGATATTTTTAATTACTTTGATTTGCT GGAACCTTCCACATGGCCTTATGAAGAAATAACTTCACCATGGATAGCTGGTGAAAAAACATTATTTCAT TTGTGTAAAATTTTAAAATATGAAGTTGATTTGAATGATTTTCGGGAATTTGTAAATAATAATATAAAAT CAAACAATGTTTCAATTCCTACAACTATATACAAAGCTAAAAAGATAGTTAGCACCATTGCAATCAATAG TGCTGAAGCTGAAAGGGGTTTCAATTTAATGAACATAATTTGTACAAGGGTGAGAAATAGTTTAACAATA GATCATGTATCAGATTTAATGACAATAAATTTACTGGGGAAAGAATTAGCAGATTGGGATGCAACACCGT TTGTAAAATCTTGGTCAAATTGCAACCACAGGTTGGCTACAGATACAAGAGTTCGGCAAAAGTCAACAAA AGTCTTCCATGAGAATCAATTGGCTATATGGAACTTAAAATAG
Restriction Sites	SgfI-MluI
ACCN	NM_001286274
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286274.1, NP_001273203.1
RefSeq Size	2883 bp
RefSeq ORF	2283 bp
Locus ID	57691
UniProt ID	Q9HCI6
Gene Summary	E3 SUMO-protein ligase; facilitates UBE2I/UBC9-mediated SUMO2 modification of target proteins (PubMed:26524493).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.