TMC7 (NM_001300732) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001300732, the custom clone sequence may differ by one or more nucleotides ATGAGCGAGTCCAGCGGCAGTGCGCTCCAGCCCGGCAGGCCCAGCCGGCAGCCGGCGGTCCATCCAGAGA ACCTCTCTCTAGACTCCAGTTGCTTCTCTTCTCCACCTGTGAACTTCCTCCAAGAATTGCCAAGCTACCG GTCCATTGCACGTAGGAGAACGACTGTCCATTCCCGGGACAAGCAAAGCGGAACTTTGCTAAAGCCAACC GACTCTTACAGCTCCCAGCTGGAGGACAGAATCGCTGAAAACCTCAGCAGCCATTCTCTTCGAAATTATG CACTGAACATCTCTGAGAAGCGGAGACTAAGGGACATTCAAGAGACACAAATGAAGTATCTCTCCGAATG GGACCAGTGGAAGCGGTATAGCAGCAAGTCTTGGAAGAGGTTCCTAGAGAAGGCTCGAGAGATGACGACC CACCTGGAGCTGTGGCGGGAGGACATCCGCAGCATAGAAGGGAAATTTGGCACTGGGATTCAGTCCTATT TCTCCTTCTTGAGATTCCTGGTGTTGCTGAATTTGGTGATATTTCTGATCATCTTTATGCTGGTTTTGCT CCCAGTCTTACTCACGAAATACAAGATCACCAACAGCAGCTTCGTGCTCATTCCTTTCAAAGACATGGAT AAACAATGTACAGTCTATCCAGTAAGCAGTTCTGGACTCATTTACTTTTACAGTTATATCATAGACTTGC TTTCTGGCACTGGTTTCCTGGAGGAAACTAGCCTCTTTTACGGACATTACACCATTGATGGGGTGAAATT TCAGAACTTCACCTATGATCTGCCCCTGGCGTATTTGTTAAGCACAATCGCCTCCCTGGCCCTGAGCCTT CTTTGGATAGTGAAAAGGTCGGTGGAAGGATTCAAAATCAACCTGATTCGGAGTGAGGAGCACTTTCAGA GTTACTGCAACAAGATATTTGCCGGCTGGGACTTCTGCATCACTAACCGCAGCATGGCGGATCTGAAGCA CAGCAGCTTGCGGTACGAGCTCCGAGCAGATCTGGAGGAAGAAAGAATGCGGCAGAAAATAGCAGAAAGG ACCTCAGAAGAAACAATACGCATTTACTCTTTGAGACTGTTTTTGAACTGTATTGTTCTGGCTGTTTTAG GGGCATGCTTTTATGCAATATACGTAGCAACTGTCTTCTCGCAAGAGCACATGAAAAAGGAAATCGACAA GATGGTTTTTGGAGAGAACCTCTTCATATTGTATCTACCGTCTATTGTGATCACGCTGGCCAATTTTATC ACCCCAATGATCTTTGCCAAGATCATCCGCTATGAGGATTATTCTCCAGGCTTTGAGATCCGTCTGACAA TCCTTAGGTGTGTCTTTATGCGGCTGGCCACCATATGTGTCCTGGTGTTCACGCTGGGCTCCAAGATCAC ATCCTGTGATGATGACACATGTGACCTTTGCGGCTACAACCAGAAACTCTACCCGTGCTGGGAGACCCAA GTTGGGCAGGAAATGTACAAGCTGATGATCTTCGACTTCATCATCATCTTGGCTGTGACACTCTTCGTGG ATTTTCCTAGAAAGCTCCTGGTGACCTACTGTTCCTCTTGCAAGCTGATTCAGTGCTGGGGGCAGCAGGA GTTTGCCATTCCTGATAACGTCCTGGGGATAGTTTACGGGCAAACCATCTGCTGGATCGGAGCCTTTTTC TCACCCCTTCTCCCTGCAATTGCAACCCTGAAATTCATTATCATCTTCTATGTGAAAGAGTGGAGTCTGC TTTACACCTGCAGACCCTCCCCCAGGCCGTTCAGAGCATCCAATTCTAATTTCTTCTTCCTGTTGGTGTT GTTGATCGGGCTGTGTTTGGCAATAATACCTCTGACAATCAGCATATCACGCATCCCTTCCTCGAAAGCC TGTGGGCCGTTCACCAACTTCAACACCACCTGGGAGGTCATCCCCAAGACGGTGAGCACCTTCCCCAGCT CGCTGCAGTCCTTCATCCATGGTGTCACATCCGAAGCCTTTGCAGTTCCTTTCTTCATGATTATTTGCCT CATCATGTTTTACTTCATTGCCTTAGCTGGAGCACACAAACGGGTGGTCATCCAGCTCCGAGAGCAGCTA TCCCTGGTAAGGAAGCATAGCTCCAGAGGGTCATGGCTGGGGACATTTACCCAGGACACTGCAAGGAAAG TGCTGTTTTCCTGGGAGGGAGTGAAATTACTGCAGCCTTCTCTCACCACCACCTCCCCAAAACTCTGCCT TGAGGCCCAGCCGCGTCCAGCTTCATTAATTCACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001300732
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001300732.1, NP_001287661.1
RefSeq Size	2738 bp
RefSeq ORF	2277 bp
Locus ID	79905
Protein Families	Transmembrane
Gene Summary	Probable ion channel.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) encodes the longest isoform (c). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.