RASA4B (NM_001277335) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001277335, the custom clone sequence may differ by one or more nucleotides ATGGCCAAGCGCAGCTCGCTGTACATCCGCATCGTGGAGGGGAAGAACCTTCCCGCCAAGGACATCACTG GCAGCAGCGACCCCTACTGCATCGTGAAGGTGGACAATGAGCCCATCATCAGGACAGCCACAGTGTGGAA GACCCTGTGCCCCTTCTGGGGTGAGGAGTACCAAGTGCACCTGCCGCCCACCTTCCACGCTGTGGCTTTC TATGTCATGGATGAGGATGCCCTCAGCCGGGACGACGTTATCGGAAAGGTCTGCCTTACAAGGGACACCA TAGCCTCTCACCCTAAGGGTTTCAGCGGGTGGGCCCACCTGACAGAGGTCGACCCCGACGAGGAGGTGCA GGGCGAGATCCACCTGCGGCTGGAAGTGTGGCCAGGGGCCCGGGCCTGCCGGCTACGCTGCTCTGTGCTG GAGGCCAGGGATCTGGCCCCAAAGGACCGCAATGGCACATCTGACCCCTTCGTCCGAGTGCGCTACAAGG GCCGGACACGGGAGACCTCGATCGTGAAGAAGTCATGCTACCCACGCTGGAATGAGACGTTTGAATTTGA GCTGCAGGAGGGGGCCATGGAGGCGCTGTGCGTGGAGGCCTGGGACTGGGACCTTGTCAGCCGAAACGAC TTCCTGGGCAAAGTGGTGATTGATGTCCAGAGACTGCGGGTGGTGCAGCAGGAGGAGGGCTGGTTCCGGC TGCAGCCCGACCAGTCCAAGAGCCGGCGGCATGACGAGGGCAACCTGGGCTCCTTGCAGCTGGAGGTGCG GCTGCGGGACGAGACGGTGCTGCCCTCCAGCTACTACCAGCCACTGGTGCACCTGCTGTGCCACGAGGTC AAGCTGGGCATGCAGGGCCCAGGGCAGCTGATCCCACTCATCGAGGAGACAACCAGCACCGAGTGTCGCC AGGACGTGGCCACGAACCTGCTCAAGCTCTTCCTGGGGCAGGGGCTGGCCAAGGACTTCCTGGACCTGCT CTTCCAGCTGGAGCTGAGTCGCACCAGTGAGACCAACACCCTGTTCCGGAGCAACTCTCTGGCCTCAAAG TCCGTGGAGTCTTTTCTGAAGGTGGCCGGGATGCAGTACCTGCACGGCGTCCTGGGCCCCATCATCAACA AGGTGTTTGAGGAGAAGAAGTACGTGGAGCTGGACCCCAGCAAAGTGGAAGTTAAGGATGTAGGGTGCTC CGGGCTGCACCGCCCGCAGACCGAGGCCGAGGTGCTGGAGCAGAGCGCGCAGACGCTGCGCGCCCACCTG GGGGCCCTGCTGAGCGCGCTCAGCCGCTCGGTTCGCGCGTGCCCCGCCGTGGTGCGCGCCACCTTCCGCC AGCTCTTCCGGCGCGTGCGCGAGCGCTTCCCCGGCGCCCAGCACGAGAATGTACCGTTCATCGCCGTCAC CAGCTTCCTGTGCCTGCGCTTCTTCTCTCCCGCCATCATGTCGCCCAAGCTCTTCCACCTGCGGGAGCGC CACGCGGACGCCCGCACCAGCCGCACCCTGCTCCTGTTGGCCAAGGCAGTCCAGAACGTGGGCAACATGG ACACGCCGGCTTCCAGGGCCAAGGAGGCTTGGATGGAGCCGCTGCAGCCCACCGTGCACCAGGGCGTGGC GCAGCTGAAGGACTTCATCACCAAGCTCGTGGACATCGAGGAGAAGGACGAGCTGGACCTGCAGCGGACG CTGAGTTTGCAGGCGCCACCTGTGAAGGAGGGGCCACTCTTCATCCACAGGACCAAGGGCAAGGGCCCCC TCATGTCCTCCTCCTTCAAGAAGCTCTACTTCTCCCTCACTACCGAGGCCCTCAGCTTCGCGAAGACGCC CAGCTCCAAGTGTGTGAATGAGCTTAACCAGTGGCTGTCTGCGCTGCGGAAGGTGAGCATCAACAACACC GGACTGCTGGGCTCCTACCACCCTGGCGTCTTCCGTGGGGACAAGTGGAGCTGCTGCCACCAAAAAGAGA AGACAGGTCAGGGCTGCGATAAGACCCGGTCACGGGTGACCCTGCAGGAGTGGAATGACCCTCTTGACCA TGACCTTGAGGCCCAGCTCATCTACCGGCACCTGCTGGGCGTGGAGGCCATGCTGTGGGAGAGGCACCGG GAGCTGAGCGGGGGCGCAGAGGCAGGCACGGTGCCCACGAGCCCTGGCAAAGTCCCCGAGGACTCATTGG CCCGGCTGCTCCGGGTGCTGCAGGACCTCCGCGAGGCCCATAGCTCCAGCCCGGCCGGCTCCCCACCCTC AGAGCCCAACTGCCTCCTGGAGCTGCAGACGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001277335
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001277335.1, NP_001264264.1
RefSeq Size	2794 bp
RefSeq ORF	2274 bp
Locus ID	100271927
Gene Summary	Ca(2+)-dependent Ras GTPase-activating protein, that may play a role in the Ras-MAPK pathway.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.