SP140 (NM_001278453) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	LYSP100; LYSP100-A; LYSP100-B
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001278453, the custom clone sequence may differ by one or more nucleotides ATGGCCCAGCAGGGCCAGCAGGGGCAGATGGCAAGTGGAGACAGCAATCTCAACTTCAGGATGGTCGCAG AGATCCAGAACGTAGAGGGTCAGAACCTGCAGGAGCAGGTTTGCCCTGAGCCCATTTTCAGGTTCTTCAG AGAAAACAAGGTGGAGATTGCAAGTGCAATAACAAGGCCATTTCCTTTCCTTATGGGCCTCCGAGACCGC TCCTTCATCTCCGAGCAGATGTATGAACATTTTCAAGAAGCTTTTAGAAACCTGGTCCCAGTGACAAGAG TGATGTATTGTGTACTCAGTGAACTGGAGAAGACATTTGGCTGGTCACATCTGGAAGCATTGTTCAGCAG GATTAACCTGATGGCCTATCCTGATTTAAACGAGATTTACAGAAGCTTCCAGAATGTATGCTATGAACAC TCACCTCTCCAAATGAATAATGTAAACGATTTAGAAGATAGACCCAGATTACTACCATATGGTAAACAAG AGAACAGCAATGCCTGTCATGAAATGGATGATATAGCAGTGCCTCAGGAAGCCTTGAGCTCCTCGCCAAG GTGTGAGCCAGGTTTCTCTTCAGAGTCTTGTGAGCAGTTAGCTCTCCCAAAGGCTGGTGGAGGAGATGCT GAAGATGCACCCAGCCTACTACCAGTGTCCTGTAAACTTGCTATACAAATAGATGAAGGAGAATCAGAAG AAATGCCCAAGTTACTGCCTTATGATACAGAAGAGACCTTTGATCTAAAAACTCCCCAAGTCACTAATGA AGGAGAACCAGAGAAGGGGCTCTGTCTACTACCAGGTGAAGGAGAAGAGGGCAGTGATGACTGTTCGGAA ATGTGTGATGGAGAAGAGCGCCAGGAAGCCTCTAGCTCCCTAGCAAGACGTGGGTCAGTGTCTAGTGAAC TAGAAAATCACCCAATGAATGAAGAAGGAGAATCAGAAGAGCTTGCTTCTAGCCTGCTATATGATAATGT ACCAGGAGCGGAGCAATCAGCATATGAAAATGAGAAGTGTTCCTGTGTCATGTGTTTCTCAGAAGAGGTG CCAGGAAGCCCAGAAGCAAGGACGGAAAGTGATCAAGCGTGTGGCACAATGGATACTGTGGATATTGCAA ACAACTCCACTTTGGGAAAACCCAAGAGGAAAAGAAGAAAAAAGAGGGGGCATGGCTGGAGCAGAATGAG AATGAGAAGGCAGGAAAACAGCCAACAAAATGATAATAGCAAAGCCGACGGCCAGGTGGTCTCCAGTGAA AAGAAGGCGAACGTGAATCTGAAAGACCTTTCCAAGATTAGGGGGAGAAAGAGAGGCAAACCTGGAACCC GCTTCACTCAGAGTGACAGAGCTGCACAGAAAAGAGTCCGATCAAGAGCTTCAAGAAAGCACAAAGATGA AACTGTGGATTTTAAGGCTCCTTTGCTTCCAGTGACCTGTGGTGGGGTGAAGGGAATTTTACATAAGAAG AAATTGCAGCAAGGAATCTTGGTGAAGTGTATACAGACTGAGGATGGAAAATGGTTCACCCCCACGGAAT TTGAAATCAAAGGAGGCCATGCAAGATCAAAGAACTGGAGGCTGAGTGTGCGCTGTGGCGGGTGGCCCCT ACGATGGCTGATGGAGAATGGATTTCTGCCTGATCCTCCAAGAATACGTTACAGGAAAAAAAAGAGAATA CTGAAGTCTCAAAACAATAGCTCAGTTGACCCTTGTATGAGAAACCTGGATGAGTGTGAGGTGTGCCGGG ACGGAGGGGAGCTGTTCTGTTGCGACACTTGTTCAAGAGTCTTCCATGAGGACTGTCACATCCCGCCTGT GGAAGCTGAGAGGACCCCGTGGAATTGCATCTTCTGCAGGATGAAGGAGTCTCCGGGAAGCCAACAGTGT TGTCAGGAATCTGAGGTCCTGGAGAGGCAGATGTGTCCTGAGGAACAGTTGAAATGTGAGTTCCTCCTCT TGAAAGTCTATTGCTGTTCTGAGAGCTCCTTTTTTGCCAAGATTCCATACTATTATTATATTAGAGAGGC GTGTCAAGGCCTGAAGGAGCCCATGTGGTTGGATAAAATCAAGAAAAGGCTGAATGAGCACGGTTACCCC CAAGTGGAGGGGTTTGTACAAGACATGCGCCTCATCTTCCAGAACCACAGGGCCTCTTACAAGTACAAGG ATTTTGGCCAAATGGGATTTAGACTGGAGGCTGAGTTTGAGAAGAATTTCAAGGAAGTGTTTGCTATTCA GGAAACAAATGGGAACAATTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001278453
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001278453.1, NP_001265382.1
RefSeq Size	2928 bp
RefSeq ORF	2262 bp
Locus ID	11262
UniProt ID	Q13342
Protein Families	Druggable Genome, Transcription Factors
Gene Summary	This gene encodes a member of the SP100 family of proteins, which are share common domains including an N-terminal homogeneously staining region domain followed by a SP100/autoimmune regulator/NucP41/P75/deformed epidermal autoregulatory factor domain, a plant homeobox zinc finger, and a bromodomain. The encoded protein is interferon-inducible and is expressed at high levels in the nuclei of leukocytes. Variants of this gene have been associated with multiple sclerosis, Crohn's disease, and chronic lymphocytic leukemia. Alternative splicing results in multiple variants. [provided by RefSeq, Aug 2016] Transcript Variant: This variant (5) lacks three alternate in-frame exons and uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 1. The resulting isoform (5) has the same N- and C-termini but is shorter compared to isoform 1.

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