ZP2 (NM_001290104) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	OOMD6; Zp-2; ZPA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001290104, the custom clone sequence may differ by one or more nucleotides ATGGCGTGCAGGCAGAGAGGAGGCTCTTGGAGTCCCTCAGGCTGGTTCAATGCAGGCTGGAGCACCTACA GGTCGATTTCTCTCTTCTTCGCCCTTGTGACTTCAGGGAACTCCATAGATGTTTCTCAGTTGGTAAATCC TGCCTTTCCAGGCACTGTCACTTGCGATGAAAGGGAAATAACAGTGGAGTTCCCAAGCAGTCCTGGCACC AAGAAATGGCATGCATCTGTGGTGGATCCTCTTGGTCTCGACATGCCGAACTGCACTTACATCCTGGACC CAGAAAAGCTCACCCTGAGGGCTACCTATGATAACTGTACCAGGAGAGTGCATGGTGGACACCAGATGAC CATCAGAGTCATGAACAACAGTGCTGCCTTAAGACACGGAGCTGTCATGTATCAGTTCTTCTGTCCAGCT ATGCAAGTAGAAGAGACCCAGGGGCTTTCAGCATCTACAATCTGCCAGAAGGATTTCATGTCTTTTTCCT TGCCACGGGTCTTCTCTGGCTTGGCTGACGACAGTAAGGGGACCAAAGTTCAGATGGGATGGAGCATTGA GGTTGGTGATGGTGCAAGAGCCAAAACTCTGACCCTGCCAGAGGCCATGAAGGAAGGCTTCAGCCTCTTG ATTGACAACCACAGGATGACCTTCCATGTGCCATTCAATGCCACTGGAGTGACTCACTATGTGCAAGGTA ACAGTCATCTCTACATGGTGTCTCTGAAGCTTACATTTATATCTCCTGGACAGAAGGTGATCTTCTCTTC ACAAGCTATTTGTGCACCAGATCCTGTGACCTGCAATGCCACACACATGACTCTCACCATACCAGAGTTT CCTGGGAAGCTTAAGTCTGTGAGCTTTGAAAACCAGAACATTGATGTGAGCCAGCTGCATGACAATGGAA TTGATCTAGAAGCAACAAATGGCATGAAATTGCATTTCAGCAAAACTCTGCTCAAAACGAAATTATCTGA AAAATGCCTACTCCATCAGTTCTACTTAGCTTCACTCAAGCTGACCTTTCTCCTTCGGCCAGAGACAGTA TCCATGGTGATCTATCCTGAGTGTCTCTGTGAGTCACCCGTTTCTATAGTTACAGGGGAGCTGTGCACCC AGGATGGGTTTATGGACGTCGAGGTCTACAGCTACCAAACACAACCAGCTCTTGACCTGGGTACTCTGAG GGTGGGAAACTCATCCTGCCAGCCTGTCTTTGAGGCTCAGTCTCAGGGGCTGGTACGGTTCCACATACCC CTGAATGGATGTGGAACGAGATATAAGTTCGAAGATGATAAAGTCGTCTATGAAAACGAAATACATGCTC TCTGGACGGATTTTCCTCCAAGCAAAATATCTAGAGACAGTGAGTTCAGGAATGACATGCTACTAAACAT CAACGTTGAAAGCCTTACTCCTCCAGTGGCCTCAGTGAAGTTGGGTCCATTTACCTTGATCCTGCAAAGC TACCCAGATAATTCCTACCAACAACCTTATGGGGAAAACGAGTACCCTCTAGTGAGATTCCTCCGCCAAC CAATTTACATGGAAGTGAGAGTCCTAAACAGGGATGACCCCAACATCAAGCTGGTCTTAGATGACTGCTG GGCGACGTCCACCATGGATCCAGACTCTTTCCCCCAGTGGAACGTTGTCGTGGATGGCTGTGCATATGAC CTGGACAACTACCAGACCACCTTCCATCCAGTCGGCTCCTCTGTGACCCATCCTGATCACTATCAGAGGT TTGACATGAAGGCTTTTGCCTTTGTATCAGAAGCCCACGTGCTCTCTAGCCTGGTCTACTTCCACTGCAG TGCCTTAATCTGTAATCGACTCTCCCCTGACTCCCCACTGTGTTCTGTGACCTGCCCTGTGTCCTCTAGG CACAGGCGAGCCACAGGGGCCACTGAAGCAGAGAAAATGACAGTCAGCCTCCCAGGACCCATTCTCCTGT TGTCAGATGACTCCTCATTCAGAGGTGTCGGCTCATCTGATCTAAAAGCAAGTGGGAGCAGTGGGGAGAA GAGTAGGAGTGAAACAGGGGAGGAGGTTGGCTCACGAGGTGCTATGGACACCAAAGGGCACAAGACTGCT GGAGATGTTGGTTCCAAAGCTGTGGCTGCTGTGGCTGCCTTTGCAGGTGTGGTGGCAACTCTAGGCTTCA TCTACTACCTGTACGAGAAAAGGACTGTGTCAAATCACTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290104
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290104.1, NP_001277033.1
RefSeq Size	2722 bp
RefSeq ORF	2211 bp
Locus ID	7783
UniProt ID	Q05996
Protein Families	Secreted Protein, Transmembrane
Gene Summary	The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed of three glycoproteins with various functions during fertilization and preimplantation development. The glycosylated mature peptide is one of the structural components of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. Female mice lacking this gene do not form a stable zona matrix and are sterile. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. The encoded protein (isoform 2) is shorter than isoform 1.

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