SLC9A7 (NM_001257291) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MRX108; NHE-7; NHE7; SLC9A6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001257291, the custom clone sequence may differ by one or more nucleotides ATGGAGCCTGGTGACGCGGCGCGCCCTGGCTCGGGTCGGGCTACCGGGGCGCCGCCGCCGCGGCTGCTGC TGCTGCCGCTGCTGCTGGGTTGGGGGCTGCGAGTCGCGGCCGCGGCCTCGGCCTCCTCCTCTGGGGCGGC GGCGGAGGACAGCAGCGCCATGGAGGAGCTCGCTACTGAGAAGGAGGCGGAGGAGAGCCACCGGCAAGAC AGCGTGAGCCTGCTCACCTTCATCCTGCTGCTCACGCTCACCATCCTCACCATCTGGCTCTTCAAGCACC GCCGGGTGCGCTTTCTGCACGAGACCGGGCTGGCCATGATCTATGGGCTCATCGTTGGGGTGATCCTGAG GTATGGTACCCCTGCTACCAGTGGCCGTGACAAATCACTCAGCTGCACTCAGGAAGACAGGGCCTTCAGT ACCTTATTAGTGAATGTCAGCGGAAAGTTCTTCGAATACACTCTGAAAGGAGAAATCAGTCCTGGCAAGA TCAACAGCGTAGAGCAGAATGATATGCTACGGAAGGTAACATTCGATCCAGAAGTATTTTTCAACATTCT TCTGCCTCCAATTATTTTTCATGCTGGATACAGCTTAAAGAAGAGACACTTTTTCAGAAATCTTGGATCT ATACTGGCCTATGCCTTCTTGGGGACTGCTGTTTCATGCTTCATTATTGGAAATCTCATGTATGGTGTGG TGAAGCTCATGAAGATTATGGGACAGCTCTCAGATAAATTTTACTACACAGATTGTCTCTTTTTTGGAGC AATCATCTCTGCCACTGACCCAGTGACTGTGCTGGCGATATTTAATGAATTGCATGCAGACGTGGATCTT TACGCACTTCTTTTTGGAGAGAGCGTCCTAAATGATGCTGTTGCCATTGTACTGTCCTCGTCTATTGTTG CCTACCAGCCAGCGGGACTGAACACTCACGCCTTTGATGCTGCTGCCTTTTTTAAGTCAGTTGGCATTTT TCTAGGTATATTTAGTGGCTCTTTTACCATGGGAGCTGTGACTGGTGTTGTGACTGCTCTAGTGACTAAG TTTACCAAACTGCACTGCTTCCCCCTGCTGGAGACGGCGCTGTTCTTCCTCATGTCCTGGAGCACGTTTC TCTTGGCAGAAGCCTGCGGATTTACAGGTGTTGTAGCTGTCCTTTTCTGTGGAATCACACAAGCTCATTA CACCTACAACAATCTGTCGGTGGAATCAAGAAGTCGAACCAAGCAGCTCTTTGAGGTGTTACATTTCCTG GCAGAGAACTTCATCTTCTCCTACATGGGCCTGGCACTGTTTACCTTCCAGAAGCACGTTTTCAGCCCCA TTTTCATCATCGGAGCTTTTGTTGCCATCTTCCTGGGCAGAGCCGCGCACATCTACCCGCTCTCCTTCTT CCTCAACTTGGGCAGAAGGCATAAGATTGGCTGGAATTTTCAACACATGATGATGTTTTCAGGCCTCAGG GGAGCAATGGCATTTGCGTTGGCCATCCGTGACACGGCATCCTATGCTCGCCAGATGATGTTCACGACCA CCCTTCTCATTGTGTTCTTCACTGTCTGGATCATTGGAGGAGGCACGACACCCATGTTGTCATGGCTTAA CATCAGAGTTGGCGTCGAGGAGCCCTCCGAAGAGGACCAGAATGAACACCACTGGCAGTACTTCAGAGTT GGTGTTGACCCCGATCAAGACCCACCACCCAACAACGACAGCTTTCAAGTCTTACAAGGGGACGGCCCAG ATTCTGCCAGAGGAAACCGGACAAAACAGGAGAGCGCATGGATATTCAGGCTGTGGTACAGCTTTGATCA CAATTACCTGAAGCCCATCCTCACACACAGTGGTCCCCCACTAACCACCACGCTCCCCGCCTGGTGTGGC TTACTAGCTCGATGTCTGACCAGTCCCCAGGTGTACGATAACCAAGAGCCACTGAGAGAGGAAGACTCTG ATTTCATCCTGACCGAAGGCGACCTGACATTGACCTACGGGGACAGCACAGTGACTGCAAATGGCTCCTC AAGTTCGCACACCGCCTCCACGAGTCTGGAGGGCAGCCGGAGAACGAAGAGCAGCTCGGAGGAAGTGCTG GAGCGAGACCTGGGAATGGGAGACCAGAAGGTTTCGAGCCGGGGCACCCGCCTAGTGTTTCCCCTGGAAG ATAATGCTTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001257291
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001257291.1, NP_001244220.1
RefSeq Size	10041 bp
RefSeq ORF	2181 bp
Locus ID	84679
Protein Families	Druggable Genome, Transmembrane
Gene Summary	This gene encodes a sodium and potassium/ proton antiporter that is a member of the solute carrier family 9 protein family. The encoded protein is primarily localized to the trans-Golgi network and is involved in maintaining pH homeostasis in organelles along the secretory and endocytic pathways. This protein may enhance cell growth of certain breast tumors. This gene is part of a gene cluster on chromosome Xp11.23. A pseudogene of this gene is found on chromosome 12. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2012] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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