NOLC1 (NM_001284388) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	NOPP130; NOPP140; NS5ATP13; P130; Srp40
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001284388, the custom clone sequence may differ by one or more nucleotides ATGGCGGACGCCGGCATTCGCCGCGTGGTTCCCAGCGACCTGTATCCCCTCGTGCTCGGCTTCCTGCGCG ATAACCAACTCTCAGAGGTGGCCAATAAGTTCGCCAAAGCGACAGGAGCTACACAGCAGGATGCCAATGC CTCTTCCCTCTTAGACATCTATAGCTTCTGGCTCAAGTCTGCCAAGGTCCCAGAGCGAAAGTTACAGGCA AATGGACCAGTGGCTAAGAAAGCTAAGAAGAAGGCCTCATCCAGTGACAGTGAGGACAGCAGCGAGGAGG AGGAGGAAGTTCAAGGGCCTCCAGCAAAGAAGGCTGCTGTACCTGCCAAGCGAGTCGGTCTGCCTCCTGG GAAGGCTGCAGCCAAAGCATCAGAGAGTAGCAGCAGTGAAGAGTCCAGTGATGATGATGATGAGGAGGAC CAAAAGAAACAGCCTGTCCAGAAGGGAGTTAAGCCCCAAGCCAAGGCAGCCAAAGCTCCTCCTAAGAAGG CCAAGAGCTCTGATTCTGATTCTGACTCAAGCTCCGAGGATGAGCCACCAAAGAACCAGAAGCCAAAGAT AACACCTGTGACAGTTAAAGCTCAGACTAAAGCCCCTCCCAAACCAGCTCGAGCAGCACCTAAAATAGCC AATGGTAAAGCAGCCAGTAGCAGCAGTAGCAGCAGCAGCAGCAGTAGCAGTGATGACTCAGAGGAGGAGA AGGCAGCAGCCACCCCCAAGAAGGTCTGGACCATAACTTCTGTCAGGGCAGAGACTGTACCTAAAAAGCA AGTTGTGGCCAAGGCCCCAGTGAAAGCAGCTACCACCCCTACCCGGAAGAGTTCTAGCAGTGAGGATTCC TCCAGTGACGAGGAAGAGGAGCAAAAAAAACCCATGAAAAATAAACCAGGTCCCTACAGTTCAGTCCCCC CGCCTTCTGCTCCCCCACCAAAGAAGTCTCTGGGAACCCAGCCTCCCAAGAAGGCTGTGGAGAAGCAGCA GCCTGTGGAAAGCAGTGAAGACAGCAGTGATGAGTCTGATTCAAGTTCTGAAGAAGAGAAGAAACCCCCA ACTAAGGCAGTAGTCTCTAAAGCAACCACTAAACCACCTCCAGCAAAGAAAGCAGCAGAGAGCTCTTCAG ACAGCTCAGACTCTGACAGCTCTGAGGATGATGAAGCTCCTTCTAAGCCAGCTGGTACCACCAAGAATTC TTCAAATAAGCCAGCTGTCACCACCAAGTCACCTGCAGTGAAGCCAGCTGCAGCCCCCAAGCAACCTGTG GGCGGTGGCCAGAAGCTTCTGACGAGAAAGGCTGACAGCAGCTCCAGTGAGGAAGAGAGCAGCTCCAGTG AGGAGGAGAAGACAAAGAAGATGGTGGCCACCACTAAGCCCAAGGCGACTGCCAAAGCAGCTCTATCTCT GCCTGCCAAGCAGGCTCCTCAGGGTAGTAGGGACAGCAGCTCTGATTCAGACAGCTCCAGCAGTGAGGAG GAGGAAGAGAAGACATCTAAGTCTGCAGTTAAGAAGAAGCCACAGAAGGTAGCAGGAGGTGCAGCCCCTT CCAAGCCAGCCTCTGCAAAGAAAGGAAAGGCTGAGAGCAGCAACAGTTCTTCTTCTGATGACTCCAGTGA GGAAGAGGAAGAGAAGCTCAAGGGCAAGGGCTCTCCAAGACCACAAGCCCCCAAGGCCAATGGCACCTCT GCACTGACTGCCCAGAATGGAAAAGCAGCTAAGAACAGTGAGGAGGAGGAAGAAGAAAAGAAAAAGGCGG CAGTGGTAGTTTCCAAATCAGGTTCATTAAAGAAGCGGAAGCAGAATGAGGCTGCCAAGGAGGCAGAGAC TCCTCAGGCCAAGAAGATAAAGCTTCAGACCCCTAACACATTTCCAAAAAGGAAGAAAGGAGAAAAAAGG GCATCATCCCCATTCCGAAGGGTCAGGGAGGAGGAAATTGAGGTGGATTCACGAGTTGCGGACAACTCCT TTGATGCCAAGCGAGGTGCAGCCGGAGACTGGGGAGAGCGAGCCAATCAGGTTTTGAAGTTCACCAAAGG CAAGTCCTTTCGGCATGAGAAAACCAAGAAGAAGCGGGGCAGCTACCGGGGAGGCTCAATCTCTGTCCAG GTCAATTCTATTAAGTTTGACAGCGAGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001284388
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001284388.1, NP_001271317.1
RefSeq Size	3977 bp
RefSeq ORF	2130 bp
Locus ID	9221
UniProt ID	Q14978
Protein Families	Stem cell - Pluripotency
Gene Summary	Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification (PubMed:10567578, PubMed:26399832). Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (PubMed:26399832). Involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus (PubMed:9016786). It has intrinsic GTPase and ATPase activities (PubMed:9016786).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

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