FSD2 (NM_001281806) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	SPRYD1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001281806, the custom clone sequence may differ by one or more nucleotides ATGGAGGAGGAATCGGGGGAGGAACTGGGGCTGGACAGGTCTACTCCCAAGGATTTCCACTTTTACCACA TGGACCTGTATGACTCTGAAGACAGACTGCACCTCTTTCCAGAAGAGAACACTAGGATGAGGAAAGTAGT CCAAGCTGAAATGGCCAATGAGTCAAGAGGAGCAGGGGATGGTAAGGCTCAAAGAGACCTTCAAGAGGAA GTGGATGAACTTGTCCACTTATATGGACTTGAAGATGATCATGAATTAGGGGATGAGTTTGTTGATGAAA ACATACCCAGAACAGGGGTTTCAGAATATCCTCCTTATATGATGAAGAGGAGAGACCCAGCCAGGGAGCA GAGAGACTGGAGACTTAGTGGAGAGGCAGCGGAGGCCGAGGACCTGGGCTTCGGAGGGTGGGGCTCAGCA GGCCAGTGCCAGGACTTGCGGGAAGCCTATAGGTACACACACGGCCGTGCCAGCGAGGAGTATGAATGCT ATGTCATCCCCGAGGAAGAGGATGAAGAAGAAGCTGCTGATGTCTTCTGTGTCACTTGTAAGACTCCAAT AAGAGCTTTTCAGAAGGTTTTTGATGAACATAAGGAGCATGAAGTGATCCCACTCAATGAAGCACTGGAA AGTGCCAAGGATGAAATTCACAAAAACATGTACAAATTGGAAAAGCAGATAATTGAGATGGAAAACTTTG CAAATCATTTGGAGGAGGTTTTCATCACTGTGGAGGAGAATTTTGGAAAACAAGAACAAAACTTTGAGTC ACATTACAACGAGATCTTGGAAACACTTGCTCAAAAATATGAGGAAAAAATACAAGCTCTAGGGGAGAAA AAGAAAGAGAAGCTGGAAGCCTTATATGGACAACTGGTCAGCTGTGGAGAAAACCTAGATACTTGCAAAG AACTGATGGAAACAATAGAGGAGATGTGTCACGAGGAGAAGGTGGATTTCATAAAGGATGCAGTGGCTAT GGCTGACAGACTCGGGAAATTCCTGAAAACAAAAACAGACGTGGAAATCTCTGCACAGCCTGAGTTTGAA GACCAGACCTTGGATTTCTCTGATGTGGAGCAGCTGATGGGCTCCATTAACACCATTCCAGCTCCTTCTG CTCCAGTCATAAATCCACAGGTCCCTAACTCAGCCACAGGTTCCTCAGTCCGAGTGTGCTGGAGCTTATA CTCCGACGACACTGTGGAGAGCTATCAGCTGTCCTACCGGCCAGTGCAGGACAGCTCACCTGGGACGGAC CAAGCAGCACCTTCTCCCCCCATTATTAAAACCAAAGAGATAAGGAGCTGTGAAGAGGCTGTGCTGATTT GCTGGGAGTCTGGGAACCTGAATCCTGTGGACTCGTACACTGTGGAGCTGACCCAGGCTGAAAGTCCAGA AGCCTCGGGTGTAACTGAGTCTGTTGTGGGCATCCCGACCTGCGAGTCCGTGGTGCAGCTGCAGCCGGGG CGGAGCTACATTATCTATGTGCGAGCCCTCAATATGGGGGGCCCCAGCGTGAGGAGCGAGCCAGCTACAG TCCACACCATAGGAAGCTACTTTCGCCTAAACAAGGACACTTGCCATCCCTGGCTGACCATTTCTGAAGA CGGACTTACGGCTGTACGAAGTGAAAGGAGAACCCCCGCCAGGGAGCTGTCACCCAGCGACACTCACTTC ACCAGGTGTGTTGCTGTCATGGGAAATCTAATTCCAGTCCGAGGGCACCATTACTGGGAGGTGGAGGTGG ATGAGCATTTGGACTACAGAGTTGGTGTGGCCTTTGCAGATGTCCGTAAACAGGAGGATCTGGGAGCAAA TTGCCTCTCCTGGTGCATGAGGCACACATTTGCATCATCAAGGCATAAGTATGAATTTCTGCACAACAGA ACAACTCCAGATATAAGAATAACAGTTCCACCAAAGAAGATTGGCATTCTATTAGACTATGAACATTCAA AGTTGTCATTTTTCAATGTGGACCTTTCTCAGCATCTATATACATTTAGTTGTCAGCTTCACGAATTTGT GCATCCCTGTTTTTCTTTGGAAAAGCCTGGGTGTCTAAAGGTACATAATGGCATTTCAATGCCAAAACAT GTCACTTTCTATTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001281806
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001281806.1, NP_001268735.1
RefSeq Size	6301 bp
RefSeq ORF	2115 bp
Locus ID	123722
UniProt ID	A1L4K1
Protein Families	Druggable Genome
Gene Summary	This gene encodes a protein that belongs to the FN3/SPRY family of proteins. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] Transcript Variant: This variant (3) lacks an in-frame exon compared to variant 1. The encoded isoform (b) is shorter than isoform a. Variants 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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