MTMR12 (NM_001294343) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	3-PAP; PIP3AP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001294343, the custom clone sequence may differ by one or more nucleotides ATGCTGGGGAAAGGAGTAGTCGGCGGTGGCGGCGGCACCAAGGCCCCCAAGCCCTCCTTCGTGTCGTACG TACGCCCTGAGGAAATTCACACAAACGAAAAGGAAGTAACAGAGAAGGAAGTAACTCTTCACTTGTTGCC AGGTGAACAGCTGCTTTGTGAAGCCAGCACAGTACTGAAGTATGTCCAGGAAGATTCCTGTCAGCATGGG GTCTATGGGAGGCTTGTCTGCACAGACTTCAAGATTGCCTTCTTGGGTGATGATGAATCTGCATTGGATA ATGATGAAACTCAATTTAAGAATAAGGTTATAGGAGAAAATGACATTACACTCCACTGTGTTGATCAGAT TTATGGAGTGTTTGATGAGAAAAAGAAAACTCTCTTTGGACAACTGAAGAAATACCCTGAGAAGCTCATC ATCCACTGCAAAGACCTTCGAGTGTTCCAGTTTTGTCTGAGGTACACAAAGGAAGAGGAAGTCAAAAGGA TTGTCAGTGGCATAATTCATCATACCCAGGCTCCTAAACTGCTTAAACGATTATTTCTGTTTTCCTATGC GACTGCTGCACAAAACAATACAGTCACTGATCCCAAGAACCATACCGTAATGTTTGACACACTTAAGGAC TGGTGTTGGGAACTGGAACGGACCAAAGGCAACATGAAGTACAAAGCAGTGAGTGTCAACGAAGGCTATA AAGTCTGTGAGAGATTGCCAGCATACTTTGTTGTCCCCACCCCTCTTCCTGAAGAGAATGTGCAGCGCTT TCAGGGTCATGGCATACCAATATGGTGTTGGTCCTGCCACAATGGAAGTGCCCTTTTGAAAATGTCAGCA CTGCCCAAAGAACAGGATGACGGCATTTTACAAATCCAAAAGAGCTTCTTAGATGGAATTTACAAGACCA TCCACAGGCCACCCTATGAAATTGTTAAAACGGAAGACCTGTCAAGCAACTTCCTGTCCCTGCAGGAAAT CCAGACTGCATACTCTAAATTTAAACAGCTATTTCTGATAGATAACAGTACTGAATTTTGGGACACAGAT ATAAAATGGTTTTCTCTGTTGGAAAGTAGCAGCTGGCTTGACATAATCAGACGTTGCCTGAAAAAAGCAA TAGAGATTACAGAATGTATGGAAGCACAAAACATGAATGTTCTTCTTTTAGAGGAGAATGCATCCGACCT CTGCTGTCTCATTTCCTCTCTGGTGCAACTGATGATGGACCCCCACTGCAGAACCAGAATTGGTTTCCAG AGCCTCATCCAAAAGGAGTGGGTCATGGGTGGCCACTGTTTCTTGGATCGCTGCAACCATCTCCGCCAGA ACGACAAAGAGGAGGTTCCTGTGTTCCTGCTTTTCCTAGATTGTGTCTGGCAGCTGGTGCACCAGCATCC CCCGGCATTTGAATTCACAGAGACTTACCTGACTGTTTTGTCAGATAGCCTGTATATACCTATTTTTAGC ACCTTCTTCTTCAATTCACCTCATCAAAAAGATACTAACATGCATCAACGACAACTTTCTTTGCCACTTA CACAATCTAAGTCATCTCCCAAAAGAGGATTTTTCAGGGAAGAAACAGATCATTTAATTAAAAACCTTCT GGGCAAGAGAATTAGCAAACTTATTAATTCTTCTGATGAGCTCCAAGACAACTTTCGAGAGTTCTATGAC AGCTGGCATAGCAAGTCCACTGACTACCATGGTTTGTTGTTACCGCATATCGAGGGGCCCGAAATCAAAG TCTGGGCCCAGCGCTACCTACGTTGGATTCCAGAAGCCCAAATCCTAGGTGGTGGCCAAGTGGCCACTCT GAGCAAACTCTTGGAAATGATGGAGGAAGTCCAGAGTTTACAAGAGAAGATCGACGAGAGACACCACAGC CAGCAGGCCCCCCAGGCTGAGGCCCCCTGCCTGCTGAGGAACTCTGCCCGCCTCTCGTCTTTGTTTCCTT TCGCTCTGCTCCAGCGACATTCCTCTAAGCCCGTCTTACCCACCAGTGGCTGGAAAGCTTTGGGAGATGA AGACGATTTGGCCAAACGAGAAGATGAGTTCGTGGACCTAGGGGATGTGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001294343
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001294343.1, NP_001281272.1
RefSeq Size	5036 bp
RefSeq ORF	2082 bp
Locus ID	54545
UniProt ID	Q9C0I1
Protein Families	Druggable Genome
Gene Summary	Phosphatidylinositide 3-kinase-derived membrane-anchored phosphatidylinositides, such as phosphatidylinositol 3-phosphate (PtdIns(3)P), regulate diverse cellular processes. The protein encoded by this gene functions as an adaptor subunit in a complex with an active PtdIns(3)P 3-phosphatase. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014] Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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