CWH43 (NM_001286791) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CWH43-C; PGAP2IP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC337173 representing NM_001286791. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGATCTATTACTTTCCTTTGCAAACACTAGAACTCACTGGGCTTGAAGGTTTTAGTATAGCATTTCTT TCTCCAATATTCCTAACAATTACTCCTTTCTGGAAATTGGTTAACAAGAAGTGGATGCTAACCCTGCTG AGGATAATCACTATTGGCAGCATAGCCTCCTTCCAGGCTCCAAATGCCAAACTTCGACTGATGGTTCTT GCGCTTGGGGTGTCTTCCTCACTGATAGTGCAAGCTGTGACTTGGTGGTCAGGAAGTCATTTGCAAAGG TACCTCAGAATTTGGGGATTCATTTTAGGACAGATTGTTCTTGTTGTTCTACGCATATGGTATACTTCA CTAAACCCAATCTGGAGTTATCAGATGTCCAACAAAGTGATACTGACATTAAGTGCCATAGCCACACTT GATCGTATTGGCACAGATGGTGACTGCAGTAAACCTGAAGAAAAGAAGACTGGTGAGGTAGCCACGGGG ATGGCCTCTAGACCCAACTGGCTGCTGGCAGGGGCTGCTTTTGGTAGCCTTGTGTTCCTCACCCACTGG GTTTTTGGAGAAGTCTCTCTTGTTTCCAGATGGGCAGTGAGTGGGCATCCACATCCAGGGCCAGATCCT AACCCATTTGGAGGTGCAGTACTGCTGTGCTTGGCAAGTGGATTGATGCTTCCATCTTGTTTGTGGTTT CGTGGTACTGGTTTGATCTGGTGGGTTACAGGAACAGCTTCAGCTGCGGGGCTCCTTTACCTGCACACA TGGGCAGCTGCTGTGTCTGGCTGTGTCTTCGCCATCTTTACTGCATCCATGTGGCCCCAAACACTTGGA CACCTTATTAACTCAGGGACAAACCCTGGGAAAACCATGACCATTGCCATGATATTTTATCTTCTAGAA ATATTTTTCTGTGCCTGGTGCACAGCTTTTAAGTTTGTCCCAGGAGGTGTCTACGCTAGAGAAAGATCA GATGTGCTTTTGGGGACAATGATGTTAATTATCGGGCTGAATATGCTATTTGGTCCTAAGAAAAACCTT GACTTGCTTCTTCAAACAAAAAACAGTTCTAAAGTGCTTTTCAGAAAGAGTGAAAAATACATGAAACTT TTTCTGTGGCTGCTTGTTGGTGTGGGATTGTTGGGATTAGGACTACGGCATAAAGCCTATGAGAGAAAA CTGGGCAAAGTGGCACCAACCAAAGAGGTCTCTGCTGCCATCTGGCCTTTCAGGTTTGGATATGACAAT GAAGGGTGGTCTAGTCTAGAAAGATCAGCTCACCTGCTCAATGAAACAGGTGCAGATTTCATAACAATT TTGGAGAGTGATGCTTCTAAGCCCTATATGGGGAACAATGACTTAACCATGTGGCTAGGGGAAAAGTTG GGTTTCTATACAGACTTTGGTCCAAGCACAAGGTATCACACTTGGGGGATTATGGCTTTGTCAAGATAC CCAATTGTGAAATCTGAGCATCACCTTCTTCCGTCACCAGAGGGCGAGATCGCACCAGCCATCACATTG ACCGTTAACATTTCGGGCAAGCTGGTGGATTTTGTCGTGACACACTTTGGGAACCACGAAGATGACCTC GACAGGAAACTGCAGGCTATTGCTGTTTCAAAACTACTGAAAAGTAGCTCTAATCAAGTGATATTTCTG GGATATATCACTTCAGCACCTGGCTCCAGAGATTATCTACAGCTCACTGAACATGGCAATGTGAAGGAT ATCGACAGCACTGATCATGACAGATGGTGTGAATACATTATGTATCGAGGGCTGATCAGGTTGGGTTAT GCAAGAATCTCCCATGCTGAACTGAGTGATTCAGAAATTCAGATGGCAAAATTTAGGATCCCTGATGAC CCCACTAATTATAGAGACAACCAGAAAGTGGTCATAGACCACAGAGAAGTTTCTGAGAAAATTCATTTT AATCCCAGATTTGGATCCTACAAAGAAGGACACAATTATGAAAACAACCATCATTTTCATATGAATACT CCCAAATACTTTTTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001286791
Insert Size	2019 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286791.1
RefSeq Size	2405 bp
RefSeq ORF	2019 bp
Locus ID	80157
UniProt ID	Q9H720
Protein Families	Transmembrane
MW	75.5 kDa
Gene Summary	Involved in lipid remodeling during GPI-anchor maturation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate first exon and a downstream start codon compared to variant 1. The resulting isoform (2) has a shorter N-terminus compared to isoform 1.

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