GGA3 (NM_001291641) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC337099 representing NM_001291641. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAGAACTGTGGGAGGAGATTTCATAACGAAGTGGGGAAGTTCCGCTTTTTGAATGAGTTAATCAAA GTCGTCTCTCCAAAGTACCTGGGGGACAGGGTGTCTGAGAAAGTGAAGACCAAGGTTATTGAGCTGCTG TACAGCTGGACCATGGCCCTGCCAGAAGAAGCAAAGATCAAAGACGCCTACCACATGCTGAAGAGACAG GGCATAGTGCAGTCTGACCCACCAATTCCTGTGGATAGGACGCTGATCCCCTCTCCACCACCTCGTCCC AAAAACCCTGTTTTTGATGATGAGGAGAAGTCCAAGCTTTTAGCCAAGCTGCTGAAAAGCAAAAACCCA GATGACCTGCAGGAGGCCAACAAGCTCATCAAGTCCATGGTGAAGGAAGACGAGGCACGGATCCAGAAG GTGACCAAGCGTCTGCACACGTTAGAGGAAGTTAACAACAACGTGAGACTGCTCAGTGAGATGCTGCTT CATTACAGCCAGGAGGACTCTTCGGACGGGGACAGAGAGCTGATGAAGGAGCTGTTTGATCAGTGTGAG AACAAGAGGCGGACTTTATTTAAACTCGCCAGTGAGACTGAGGACAATGATAACAGTTTGGGGGACATC CTGCAAGCCAGTGACAACCTCTCCCGGGTCATCAACTCTTACAAAACAATTATTGAAGGGCAGGTCATC AATGGCGAGGTGGCTACCTTAACCCTGCCTGACTCGGAAGGAAACAGTCAGTGCAGTAACCAAGGCACG CTCATCGACCTTGCGGAGCTGGACACGACCAACAGTTTGTCCTCCGTGTTGGCCCCAGCACCTACTCCA CCCTCCTCAGGCATCCCAATCCTCCCTCCACCACCCCAGGCCTCAGGACCTCCACGGAGCCGCTCCTCT AGCCAGGCCGAGGCCACCCTGGGGCCCAGCAGCACAAGCAACGCCCTCTCCTGGCTGGACGAGGAGCTA CTCTGCTTGGGCCTCGCCGACCCAGCCCCTAATGTTCCTCCCAAAGAGTCAGCTGGGAACAGCCAGTGG CACCTGCTCCAGAGGGAACAGTCCGACCTGGACTTCTTCAGCCCCAGGCCGGGGACCGCTGCCTGTGGC GCCTCCGATGCTCCTCTGCTCCAGCCCTCAGCCCCCTCCTCAAGCAGCTCCCAAGCTCCACTGCCGCCT CCCTTCCCAGCTCCTGTGGTCCCAGCCAGTGTTCCTGCCCCCAGTGCGGGCTCCTCCTTGTTTTCTACT GGAGTGGCCCCAGCCTTGGCCCCAAAAGTTGAGCCCGCAGTCCCTGGGCACCATGGCTTGGCGTTGGGC AACAGCGCGCTGCACCACCTGGATGCCCTCGATCAGCTTCTAGAAGAGGCCAAAGTGACCTCGGGCTTG GTGAAACCCACTACCTCCCCTCTCATCCCCACCACCACCCCAGCCAGGCCCCTCCTGCCCTTCTCCACG GGGCCCGGCAGCCCGCTCTTCCAGCCACTGAGTTTCCAGTCCCAGGGCAGCCCCCCGAAGGGGCCTGAG CTCTCCCTGGCCAGCATCCACGTGCCCCTGGAATCGATCAAGCCTAGCAGTGCCCTTCCTGTGACAGCC TACGATAAAAACGGCTTCCGCATCCTCTTCCACTTTGCCAAGGAGTGTCCCCCAGGACGACCTGACGTG CTGGTGGTGGTGGTGTCCATGCTGAACACGGCTCCCTTACCTGTCAAGAGCATCGTGCTGCAGGCTGCA GTGCCCAAGTCAATGAAAGTGAAGTTGCAGCCACCTTCTGGGACAGAACTCTCTCCATTTAGCCCCATC CAGCCACCTGCAGCCATCACCCAGGTCATGTTGCTGGCCAATCCACTGAAGGAGAAGGTGCGGCTTCGG TATAAGCTGACCTTCGCCCTGGGGGAGCAGCTGAGCACAGAGGTGGGCGAGGTGGACCAGTTCCCTCCT GTGGAACAGTGGGGGAACCTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001291641
Insert Size	1956 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291641.1
RefSeq Size	4118 bp
RefSeq ORF	1956 bp
Locus ID	23163
UniProt ID	Q9NZ52
Protein Families	Druggable Genome
Protein Pathways	Lysosome
MW	70.1 kDa
Gene Summary	This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants have been identified in this gene. [provided by RefSeq, Feb 2010] Transcript Variant: This variant (3) contains an additional exon in the 5' region, and it thus differs in its 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant long. The encoded isoform (1) is shorter at the N-terminus, compared to isoform long. Both variants 1 and 3 encode isoform 1.

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