SLC28A1 (NM_001287762) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CNT1; HCNT1; URCTU
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC337094 representing NM_001287762. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGAACGACCCCTCGAGACGAAGAGAGTCCATCTCTCTCACACCTGTGGCCAAGGGTCTGGAGAAC ATGGGGGCTGATTTCTTGGAAAGCCTGGAGGAAGGCCAGCTCCCTAGGAGTGACTTGAGCCCCGCAGAG ATCAGGAGCAGCTGGAGCGAGGCGGCGCCGAAGCCCTTCTCCAGATGGAGGAACCTGCAGCCAGCCCTG AGAGCCAGAAGCTTCTGCAGGGAGCACATGCAGCTGTTTCGATGGATCGGCACAGGCCTGCTCTGCACT GGGCTCTCTGCCTTCCTGCTGGTGGCCTGCCTCCTGGATTTCCAGAGGGCCCTGGCTCTGTTTGTCCTC ACCTGTGTGGTCCTCACCTTCCTGGGCCACCGCCTGCTGAAACGGCTTCTGGGGCCAAAGCTGAGGAGG TTTCTCAAGCCTCAGGGCCATCCCCGCCTGCTGCTCTGGTTTAAGAGGGGTCTAGCTCTTGCTGCTTTC CTGGGCCTGGTCCTGTGGCTGTCTCTGGACACCTCCCAGCGGCCTGAGCAACTGGTGTCCTTCGCAGGA ATCTGCGTGTTCGTCGCTCTCCTCTTTGCCTGCTCAAAGCATCATTGCGCAGTGTCCTGGAGGGCCGTG TCTTGGGGACTTGGACTGCAGTTTGTACTTGGACTCCTCGTCATCAGAACAGAACCAGGATTCATTGCG TTCGAGTGGCTGGGCGAGCAGATCCGGATCTTCCTGAGCTACACGAAGGCTGGCTCCAGCTTCGTGTTT GGGGAGGCGCTGGTCAAGGATGTCTTTGCCTTTCAGGTTCTGCCCATCATTGTCTTTTTCAGCTGTGTC ATATCCGTTCTCTACCACGTGGGCCTCATGCAGTGGGTGATCCTGAAGATTGCCTGGCTGATGCAAGTC ACCATGGGCACCACAGCCACTGAGACCCTGAGTGTGGCTGGAAACATCTTTGTGAGCCAGACCGAGGCT CCATTACTGATCCGGCCCTACTTGGCAGACATGACACTCTCTGAAGTCCACGTTGTCATGACCGGAGGT TACGCCACCATTGCTGGCAGCCTGCTGGGTGCCTACATCTCCTTTGGGATCGATGCCACCTCGTTGATT GCAGCCTCTGTGATGGCTGCCCCTTGTGCCTTGGCCCTCTCCAAGCTGGTCTACCCGGAGGTGGAGGAG TCCAAGTTTAGGAGGGAGGAAGGAGTGAAACTGACCTATGGAGATGCTCAGAACCTCATAGAAGCAGCC AGCACTGGGGCCGCCATCTCCGTGAAGGTGGTCGCCAACATCGCTGCCAACCTGATTGCGTTCCTGGCT GTGCTGGACTTTATCAATGCTGCCCTCTCCTGGCTGGGAGACATGGTGGACATCCAAGGGCTCAGCTTC CAGCTCATCTGCTCCTACATCCTGCGGCCTGTAGCCTTCTTGATGGGTGTGGCGTGGGAGGACTGCCCA GTGGTAGCTGAGCTGCTGGGGATCAAGCTGTTTCTGAACGAGTTTGTGGCCTATCAAGACCTCTCCAAG TACAAGCAACGCCGCCTGGCAGGGGCCGAGGAGTGGGTCGGCGACAGGAAGCAGTGGATCTCCGTCAGA GCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCCAATTTCAGCTCCATTGGGATCATGCTGGGA GGCTTGACCTCCATGGTCCCCCAACGGAAGAGCGACTTCTCCCAGATAGTGCTCCGGGCGCTCTTCACG GGAGCCTGTGTGTCCCTGGTGAACGCCTGTATGGCAGGGATCCTCTACATGCCCAGGGGGGCTGAAGTT GACTGCATGTCCCTCTTGAACACGACCCTCAGCAGCAGTAGCTTTGAGATTTACCAGTGCTGCCGTGAG GCCTTCCAGAGCGTCAATCCAGAGTTCAGCCCAGAGGCCCTGGACAACTGCTGTCGGTTTTACAACCAC ACGATCTGTGCACAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001287762
Insert Size	1950 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001287762.1
RefSeq Size	2653 bp
RefSeq ORF	1950 bp
Locus ID	9154
UniProt ID	O00337
Protein Families	Transmembrane
MW	71.6 kDa
Gene Summary	Sodium-dependent and pyrimidine-selective. Exhibits the transport characteristics of the nucleoside transport system cit or N2 subtype (N2/cit) (selective for pyrimidine nucleosides and adenosine). It also transports the antiviral pyrimidine nucleoside analogs 3'-azido-3'-deoxythymidine (AZT) and 2',3'-dideoxycytidine (ddC). It may be involved in the intestinal absorption and renal handling of pyrimidine nucleoside analogs used to treat acquired immunodeficiency syndrome (AIDS). It has the following selective inhibition: adenosine, thymidine, cytidine, uridine >> guanosine, inosine.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) lacks an exon in the 5' UTR, compared to variant 1, and encodes isoform 1. Variants 1 and 4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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