ODAD2 (NM_001290021) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARMC4; CILD23; gudu
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336980 representing NM_001290021. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGGGATTTCAGCTTAGCTCAAGAAACCTGCCAGTTGGCCATCAGAGATGTTGGAGGCCTGGAAGTG CTGATAAATTTGCTTGAAACCGATGAAGTCAAATGTAAGATTGGTTCATTAAAAATACTGAAGGAAATC AGTCATAATCCTCAAATCAGACAGAATATTGTTGACCTTGGGGGCTTACCAATTATGGTGAATATACTT GATTCTCCACACAAGAGTCTAAAATGTTTGGCAGCCGAGACTATCGCGAATGTTGCCAAGTTTAAAAGA GCACGGCGGGTGGTGAGGCAGCACGGGGGTATCACCAAACTGGTTGCTCTACTAGACTGTGCACATGAT TCCACAAAACCTGCCCAATCGAGTCTGTATGAGGCCAGAGACGTGGAAGTGGCTCGCTGTGGGGCACTG GCCCTGTGGAGCTGCAGTAAGAGTCATACGAATAAAGAAGCCATCCGCAAAGCTGGGGGCATTCCTCTG TTGGCTCGGCTGCTGAAGACTTCTCATGAAAACATGCTAATTCCAGTGGTGGGGACATTGCAAGAGTGT GCATCAGAGGAAAACTACCGGGCTGCAATCAAAGCAGAAAGGATCATTGAAAACCTTGTCAAGAACCTA AATAGTGAGAATGAGCAGCTGCAGGAGCACTGCGCCATGGCCATTTACCAGTGTGCTGAAGATAAGGAA ACCCGGGACCTCGTTAGGCTGCACGGAGGACTTAAGCCCTTGGCCAGTCTACTCAATAACACTGACAAT AAAGAGCGGTTAGCTGCTGTCACAGGGGCTATATGGAAATGTTCCATCAGCAAAGAGAATGTTACCAAG TTTCGGGAATACAAAGCCATTGAAACCTTGGTGGGACTTCTAACAGATCAGCCTGAAGAAGTACTTGTG AATGTGGTTGGGGCCTTGGGAGAATGCTGCCAAGAACGTGAAAACCGAGTCATTGTCCGGAAATGTGGT GGCATTCAACCACTTGTGAACCTCCTTGTTGGAATAAACCAAGCTCTTCTTGTGAATGTTACAAAAGCA GTTGGTGCTTGTGCAGTAGAACCTGAAAGTATGATGATAATTGATCGCTTAGATGGAGTTCGTTTGTTG TGGTCCCTGCTGAAAAATCCTCACCCAGACGTGAAGGCCAGCGCAGCATGGGCACTCTGTCCATGCATC AAAAATGCAAAGGATGCTGGGGAAATGGTTCGTTCCTTTGTTGGTGGTTTGGAACTTATTGTCAATTTA CTGAAATCAGATAACAAAGAAGTTCTGGCAAGTGTATGTGCTGCCATTACCAACATAGCAAAAGATCAA GAAAATTTAGCTGTTATCACAGATCATGGAGTTGTTCCTTTATTGTCCAAACTGGCAAATACAAATAAC AATAAATTGAGACATCATCTAGCAGAAGCTATTTCACGTTGCTGTATGTGGGGCAGGAATAGAGTGGCC TTCGGTGAGCACAAAGCAGTGGCTCCACTAGTGCGTTATCTGAAATCAAATGACACCAACGTGCATCGG GCGACAGCTCAGGCCTTGTACCAACTCTCAGAAGACGCCGATAACTGCATCACCATGCATGAGAATGGT GCAGTAAAGACAGAGTCTCGCCATGTCGCCAGGCTAGAGTGCAGTGACAACGATCTTGGCTCACTGCAA CCTCCACCTCCCGGATTCAAGCGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGCTTCTAC TGGATATGGTTGGGTCCCCTGACCAGGATCTCCAGGAAGCTGCAGCTGGTTGTATATCCAATATCCGCA GGCTGGCTCTTGCTACAGAGAAGGCAAGATACACTTGAAATTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001290021
Insert Size	1839 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290021.1
RefSeq Size	2585 bp
RefSeq ORF	1839 bp
Locus ID	55130
UniProt ID	Q5T2S8
MW	67.6 kDa
Gene Summary	The protein encoded by this gene contains ten Armadillo repeat motifs (ARMs) and one HEAT repeat, and is thought to be involved in ciliary and flagellar movement. This protein has been shown to localize to the ciliary axonemes and at the ciliary base of respiratory cells. Studies indicate that mutations in this gene cause partial outer dynein arm (ODA) defects in respiratory cilia. The cilia of cells with mutations in this gene displayed either reduced ciliary beat frequency and amplitude, or, complete immotility. Some individuals with primary ciliary dyskensia (PCD) have been shown to have mutations in this gene. PCD is characterized by chronic airway disease and left/right body asymmetry defects. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, causing translation initiation at a downstream start codon, and contains an alternate exon in the 3' coding region, which results in a frameshift and a slightly shorter 3' UTR, compared to variant 1. The encoded isoform (2) has a shorter N-terminus and a distinct C-terminus compared to isoform 1.

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