GAS2L3 (NM_001303131) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	G2L3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336899 representing NM_001303131. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGAAAAGTGCCCTGTAAGAAAGATGCTGCATCAGGTTCATTCTTTGCTCGGGACAATACCGCAAAC TTCCTTCACTGGTGTAGGGACATTGGGGTTGATGAAACTTACCTCTTTGAATCTGAAGGTTTAGTTTTG CACAAAGATCCAAGACAGGTGTATCTTTGTCTTCTTGAAATTGGTCGAATTGTGTCAAGATACGGGGTT GAGCCACCAGTGTTAGTAAAACTTGAGAAAGAAATTGAGTTAGAAGAGACTTTGCTTAATACTTCTGGG CCTGAAGATTCCATCAGCATTCCAAAATCATGCTGTCGGCATGAAGAGCTACATGAAGCTGTTAAACAT ATTGCTGAGGACCCTCCTTGTAGTTGTTCTCATCGATTTTCTATTGAGTATTTATCTGAAGGACGGTAC CGACTAGGGGATAAAATACTCTTTATAAGAATGCTTCATGGAAAACATGTCATGGTTCGCGTTGGTGGA GGCTGGGATACTCTTCAAGGATTTTTGCTTAAATATGACCCCTGTCGAATATTACAGTTTGCCACATTA GAACAAAAAATTTTAGCATTTCAAAAAGGAGTTTCTAATGAAAGTGTACCTGATTCGCCTGCCAGAACA CCTCAGCCTCCTGAAATGAATCCTTTGTCAGCAGTTAACATGTTTCAGAAACAAAATTCAAAACCCAGC GTGCCAGTTAGTATTCCAAAAAGCAAAGAAAAACAGGGACGTCCACCAGGTGCATTGGTGCCAGCATCT TCACTGAAAGGAGGTAATCTGGGCTCTATGTCAGTCCGTTCTAAATTGCCAAATTCTCCAGCAGCATCT TCTCATCCCAAGCTCAAGTCTTCAAAAGGCATAACGAAGAAACCGCAGGCTCCTTCAAACAATGCATCA TCTTCACTTGCTTCATTAAATCCAGTAGGTAAAAACACTTCTTCACCAGCTTTACCAAGAACTGCACCT TGTATATCTGAGTCACCGAGAAAATGTATTTCATCCCCCAATACCCCCAAGGCCAAGGTTATTCCAGCC CAGAATTCAGCAGATCTGCCCGAGTCCACACTTTTGCCAAATAAGTGTTCAGGAAAAACTCAACCTAAG TATTTGAAACATAATCATATTTCTTCCAGAGATAATGCAGTATCTCACTTAGCTGCACATTCAAATTCA TCCTCAAAATGTCCCAAGCTGCCTAAAGCAAATATACCTGTAAGACCTAAACCTTCTTTCCAGTCCTCT GCAAAAATGACAAAAACCAGTTCCAAAACCATAGCCACGGGTCTAGGAACACAGTCTCAACCATCCGAT GGAGCCCCACAAGCAAAGCCAGTCCCAGCACAGAAACTTAAATCGGCCTTGAATTTAAATCAGCCAGTT TCTGTGTCCTCAGTTTCTCCTGTAAAAGCCACACAGAAATCAAAAGATAAGAATATAGTTTCAGCTACC AAAAAGCAGCCTCAGAATAAAAGTGCATTTCAGAAGACAGGACCCAGCTCCTTGAAGTCTCCTGGCCGT ACCCCACTGTCCATCGTGAGCCTACCCCAGTCTTCTACCAAAACACAAACTGCACCGAAGTCAGCACAG ACTGTCGCTAAGAGCCAGCATTCAACTAAAGGGCCTCCCAGAAGTGGCAAAACCCCAGCTTCAATCAGG AAACCACCCTCATCTGTTAAGGATGCAGATAGTGGAGATAAAAAACCTACTGCAAAGAAAAAGGAAGAT GATGACCATTATTTTGTCATGACTGGAAGTAAGAAACCTAGAAAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001303131
Insert Size	1773 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001303131.1
RefSeq Size	6131 bp
RefSeq ORF	1773 bp
Locus ID	283431
MW	63.6 kDa
Gene Summary	Cytoskeletal linker protein. May promote and stabilize the formation of the actin and microtubule network.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) has multiple differences compared to variant 1. These differences result in a distinct 5' UTR and cause translation initiation at a downstream start codon compared to variant 1. The encoded protein (isoform b) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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