FIGNL1 (NM_001287496) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336802 representing NM_001287496. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGTAGTGTACAGAAGATGATGCAAGCTGGCAAAAAATTCAAAGACTCTCTGTTGGAACCTGCTCTT GCATCAGTGGTAATCCATAAGGAGGCCACTGTCTTTGATCTTCCTAAATTTAGTGTTTGTGGTAGTTCT CAAGAGAGTGACTCATTACCTAACTCAGCTCATGATCGAGACCGGACCCAAGACTTCCCGGAGAGCAAT CGTTTGAAACTCCTTCAGAATGCCCAGCCACCTATGGTGACTAACACTGCTAGGACTTGTCCTACATTC TCAGCACCTGTAGGTGAGTCAGCTACTGCAAAATTCCATGTCACACCATTGTTTGGAAATGTCAAAAAG GAAAATCACAGCTCTGCAAAAGAAAACATAGGACTTAATGTGTTCTTATCTAACCAGTCTTGTTTTCCT GCTGCCTGTGAAAATCCACAGAGGAAGTCTTTTTATGGTTCTGGCACCATTGATGCACTTTCCAATCCA ATACTGAATAAGGCTTGTAGTAAAACAGAAGATAATGGCCCAAAGGAGGATAGCAGCCTGCCTACATTT AAAACTGCAAAAGAACAATTATGGGTAGATCAGCAAAAAAAGTACCACCAACCTCAGCGTGCATCAGGG TCTTCATATGGTGGTGTAAAAAAGTCTCTAGGAGCTAGTAGATCCCGAGGGATACTTGGAAAGTTTGTT CCTCCTATACCCAAGCAAGATGGGGGAGAGCAGAATGGAGGAATGCAATGTAAGCCTTATGGGGCAGGA CCTACAGAACCAGCACATCCAGTTGATGAGCGTCTGAAGAACTTGGAGCCAAAGATGATTGAACTTATT ATGAATGAGATTATGGATCATGGACCTCCAGTAAATTGGGAAGATATTGCAGGAGTAGAATTTGCTAAA GCCACCATAAAGGAAATAGTTGTGTGGCCCATGTTGAGGCCAGACATCTTTACTGGTTTAAGGGGACCC CCTAAAGGAATTTTGCTCTTTGGTCCTCCTGGGACTGGTAAAACTCTAATTGGCAAGTGCATTGCTAGT CAGTCTGGGGCAACATTCTTTAGCATCTCTGCTTCATCCTTAACTTCTAAATGGGTAGGTGAGGGGGAG AAAATGGTCCGTGCATTGTTTGCTGTTGCAAGGTGTCAGCAACCAGCTGTGATATTTATTGACGAAATT GATTCCTTGTTATCTCAACGGGGAGATGGTGAGCATGAATCTTCTAGAAGGATAAAAACAGAATTTTTA GTTCAATTAGATGGAGCAACAACATCTTCTGAAGATCGTATCCTAGTGGTGGGAGCAACAAATCGGCCA CAAGAAATTGATGAGGCTGCCCGGAGAAGATTGGTGAAAAGGCTTTATATTCCCCTCCCAGAAGCTTCA GCCAGGAAACAGATAGTAATTAATCTAATGTCCAAAGAGCAGTGTTGCCTCAGTGAAGAAGAAATTGAA CAGATTGTACAGCAGTCTGATGCGTTTTCAGGAGCAGACATGACACAGCTTTGCAGGGAGGCTTCTCTT GGTCCTATTCGCAGTTTACAAACTGCTGACATTGCTACCATAACACCGGATCAAGTTCGACCCATAGCT TACATTGATTTTGAAAATGCTTTTAGAACTGTGCGACCTAGTGTTTCTCCAAAAGATTTAGAGCTTTAT GAAAACTGGAACAAAACTTTTGGTTGTGGAAAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001287496
Insert Size	1692 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001287496.1
RefSeq Size	3271 bp
RefSeq ORF	1692 bp
Locus ID	63979
UniProt ID	Q6PIW4
MW	61.7 kDa
Gene Summary	This gene encodes a member of the AAA ATPase family of proteins. The encoded protein is recruited to sites of DNA damage where it plays a role in DNA double-strand break repair via homologous recombination. This protein has also been shown to localize to the centrosome and inhibit ciliogenesis, and may regulate the proliferation and differentiation of osteoblasts. [provided by RefSeq, Oct 2016] Transcript Variant: This variant (7) differs in the 5' UTR and includes additional splice sites in the 5' coding region resulting in translation initiation from a downstream start codon, compared to variant 3. It encodes isoform 2, which is shorter at the N-terminus, compared to isoform 1. Variants 7-9 encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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