MEPE (NM_001291183) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	OF45
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336779 representing NM_001291183. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCGAGTTTTCTGTGTGGGACTACTCCTTTTCAGTGTGACCTGGGCAGCACCAACATTTCAACCACAG ACTGAGAAAACTAAGCAAAGCTGTGTGGAAGAGCAGAGGATAACATACAAGGGTCACTATGAGAAACAT GGGCATTATGTTTTTAAGTGTGTTTACATGTCACCTGAGAAGAAAAATCAAACTGATGTAAAGCAGGAA GAAAAAAACAAAGACAATATTGGTTTTCACCATTTGGGCAAGAGAATAAATCAAGAGCTATCATCTAAA GAAAATATTGTCCAGGAAAGAAAGAAAGATTTGTCCCTTTCTGAAGCCAGTGAGAATAAGGGAAGTAGT AAATCTCAAAATTATTTCACAAATAGACAGAGACTGAATAAAGAATATAGTATCAGTAACAAAGAGAAT ACTCACAATGGCCTGAGGATGTCAATTTATCCTAAGTCAACTGGGAATAAAGGGTTTGAGGATGGAGAT GATGCTATCAGCAAACTACATGACCAAGAAGAATATGGCGCAGCTCTCATCAGAAATAACATGCAACAT ATAATGGGGCCAGTGACTGCGATTAAACTCCTGGGGGAAGAAAACAAAGAGAACACACCTAGGAATGTT CTAAACATAATCCCAGCAAGTATGAATTATGCTAAAGCACACTCGAAGGATAAAAAGAAGCCTCAAAGA GATTCCCAAGCCCAGAAAAGTCCAGTAAAAAGCAAAAGCACCCATCGTATTCAACACAACATTGACTAC CTAAAACATCTCTCAAAAGTCAAAAAAATCCCCAGTGATTTTGAAGGCAGCGGTTATACAGATCTTCAA GAGAGAGGGGACAATGATATATCTCCTTTCAGTGGGGACGGCCAACCTTTTAAGGACATTCCTGGTAAA GGAGAAGCTACTGGTCCTGACCTAGAAGGCAAAGATATTCAAACAGGGTTTGCAGGCCCAAGTGAAGCT GAGAGTACTCATCTTGACACAAAAAAGCCAGGTTATAATGAGATCCCAGAGAGAGAAGAAAATGGTGGA AATACCATTGGAACTAGGGATGAAACTGCGAAAGAGGCAGATGCTGTTGATGTCAGCCTTGTAGAGGGC AGCAACGATATCATGGGTAGTACCAATTTTAAGGAGCTCCCTGGAAGAGAAGGAAACAGAGTGGATGCT GGCAGCCAAAATGCTCACCAAGGGAAGGTTGAGTTTCATTACCCTCCTGCACCCTCAAAAGAGAAAAGA AAAGAAGGCAGTAGTGATGCAGCTGAAAGTACCAACTATAATGAAATTCCTAAAAATGGCAAAGGCAGT ACCAGAAAGGGTGTAGATCATTCTAATAGGAACCAAGCAACCTTAAATGAAAAACAAAGGTTTCCTAGT AAGGGCAAAAGTCAGGGCCTGCCCATTCCTTCTCGTGGTCTTGATAATGAAATCAAAAACGAAATGGAT TCCTTTAATGGCCCCAGTCATGAGAATATAATAACACATGGCAGAAAATATCATTATGTACCCCACAGA CAAAATAATTCTACACGGAATAAGGGTATGCCACAAGGGAAAGGCTCCTGGGGTAGACAACCCCATTCC AACAGGAGGTTTAGTTCCCGTAGAAGGGATGACAGTAGTGAGTCATCTGACAGTGGCAGTTCAAGTGAG AGCGATGGTGACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001291183
Insert Size	1671 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291183.1
RefSeq Size	2054 bp
RefSeq ORF	1671 bp
Locus ID	56955
UniProt ID	Q9NQ76
Protein Families	Secreted Protein
MW	62.2 kDa
Gene Summary	This gene encodes a secreted calcium-binding phosphoprotein that belongs to the small integrin-binding ligand, N-linked glycoprotein (SIBLING) family of proteins. Members of this family are components of the extracellular matrix of bone and dentin and regulate bone mineralization. Deficiency of a similar protein in mouse results in increased bone mass. Mice lacking this gene are resistant to aging-related trabecular bone loss. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014] Transcript Variant: This variant (6) encodes the longest isoform (c). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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