RCBTB2 (NM_001286830) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CHC1L; RLG
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336778 representing NM_001286830. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCCGCTCTTCCCCGTGATTTGGAACTTTCCAATCCCAGAGAAAAGTTGACAAAGCCAGTACAGGCT ACTCTGTCATCTTTGAAGATGTTAGATGTGGGAAAGTGGCCAATTTTTTCCCTTTGTTCTGAAGAAGAA CTACAGTTAATTCGTCAGGCTTGTGTCTTTGGCAGTGCTGGCAATGAAGTTTTATACACTACAGTAAAT GATGAGATTTTTGTGCTTGGCACAAACTGCTGTGGCTGTTTGGGGTTAGGTGACGTCCAGAGCACCATT GAACCTCGGAGACTGGATTCTTTAAATGGCAAAAAAATAGCCTGCCTCAGCTATGGGAGTGGTCCACAT ATTGTCCTTGCAACAACAGAAGGAGAAGTCTTTACCTGGGGTCATAATGCTTATAGCCAGCTGGGCAAT GGGACAACTAATCATGGTTTAGTGCCCTGTCATATCTCTACTAATCTGTCAAACAAACAAGTCATTGAA GTTGCCTGTGGGTCTTACCATTCTTTGGTGCTAACATCTGATGGAGAGGTATTTGCCTGGGGTTATAAT AACTCTGGGCAGGTAGGATCTGGATCAACAGTTAATCAGCCAATCCCTCGAAGAGTCACTGGCTGCCTA CAAAATAAAGTAGTTGTGACCATAGCATGTGGGCAGATGTGCTGCATGGCAGTAGTAGACACGGGGGAG GTCTATGTCTGGGGTTACAACGGAAACGGGCAGCTTGGACTCGGCAACAGTGGCAACCAGCCAACCCCT TGCAGAGTGGCAGCTTTGCAAGGCATCCGTGTCCAGAGGGTCGCCTGTGGCTACGCACACACATTAGTA TTAACAGATGAAGGCCAAGTGTATGCTTGGGGCGCCAATTCTTATGGGCAGTTGGGCACTGGCAATAAA AGCAACCAGTCCTATCCTACTCCTGTCACTGTGGAAAAGGACAGGATTATCGAGATTGCAGCCTGTCAC TCCACACACACGTCTGCGGCCAAGACGCAGGGTGGGCACGTGTACATGTGGGGCCAGTGCCGGGGTCAG TCCGTGATCCTCCCGCACCTCACCCACTTCTCCTGCACTGACGACGTGTTTGCCTGCTTTGCCACGCCC GCCGTCACGTGGCGCCTCCTCTCCGTGGAACCTGATGACCACCTCACAGTGGCTGAGTCACTGAAGAGG GAATTTGACAACCCGGACACTGCAGACCTGAAGTTTCTAGTTGATGGAAAGTACATTTATGCACATAAA GTCCTTCTCAAGATTCGATGTGAGCATTTTCGTTCGTCATTGGAAGATAACGAGGATGATATTGTAGAA ATGAGTGAATTTTCATATCCTGTTTACCGGGCCTTCCTGGAATACCTATACACAGACAGCATCAGCCTT TCTCCTGAGGAGGCAGTAGGACTGCTAGACTTGGCTACATTTTATAGAGAAAATCGTTTGAAAAAGCTC TGCCAACAAACTATCAAGCAAGGCATCTGCGAGGAGAATGCCATCGCTCTGCTCTCGGCTGCGGTGAAG TATGATGCACAGGATTTAGAAGAATTCTGCTTCAGGTTTTGCATAAACCATCTGACTGTAGTAACACAA ACATCAGGTTTTGCAGAAATGGACCATGATCTCCTGAAGAACTTTATCAGCAAAGCAAGCAGAGTTGGA GCCTTTAAAAATTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001286830
Insert Size	1671 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286830.1
RefSeq Size	3215 bp
RefSeq ORF	1671 bp
Locus ID	1102
UniProt ID	O95199
MW	61 kDa
Gene Summary	This gene encodes a protein containing two C-terminal BTB/POZ domains that is related to regulator of chromosome condensation (RCC). The encoded protein may act as a guanine nucleotide exchange factor. This gene is observed to be lost or underexpressed in prostate cancers. There is a pseudogene of this gene on chromosome 10. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013] Transcript Variant: This variant (1) encodes the longest isoform (1).

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