Dynamin 3 (DNM3) (NM_001278252) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	Dyna III
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336775 representing NM_001278252. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGGAACCGGGAGATGGAGGAGCTGATCCCGCTGGTGAACCGTCTGCAGGACGCGTTTTCGGCGCTG GGACAGAGCTGCCTGCTGGAGCTGCCGCAGATCGCCGTGGTGGGCGGCCAGAGCGCCGGCAAGAGCTCG GTGCTCGAGAACTTCGTGGGCAGGGACTTTCTCCCTCGAGGGTCGGGCATTGTAACAAGACGACCTCTT GTGCTGCAGCTTGTTACTTCTAAAGCAGAATATGCCGAGTTTCTACATTGCAAAGGAAAGAAATTTACA GATTTTGATGAAGTTCGCCTTGAGATTGAAGCAGAAACAGATCGCGTGACTGGAATGAATAAAGGCATT TCCTCCATACCCATTAATTTACGAGTCTATTCCCCACACGTGTTAAATCTAACCCTTATTGATCTACCT GGAATAACTAAAGTGCCTGTGGGAGATCAGCCACCAGATATCGAGTATCAGATCAGAGAAATGATTATG CAGTTCATCACGAGGGAGAACTGTCTGATTTTAGCTGTTACTCCAGCCAACACTGATCTTGCAAACTCA GATGCGCTGAAGCTAGCTAAAGAAGTTGATCCTCAAGGTCTGAGAACCATTGGAGTTATCACCAAACTG GACCTTATGGATGAAGGAACGGATGCCAGGGATGTTCTAGAGAACAAACTGTTGCCTCTTCGCAGGGGT TACGTGGGGGTGGTAAACAGAAGCCAGAAGGACATAGATGGGAAGAAGGACATAAAGGCAGCCATGCTG GCAGAGAGGAAGTTTTTCCTTTCCCACCCGGCTTACAGACATATCGCTGACCGAATGGGAACCCCACAC CTGCAGAAGGTCCTTAATCAGCAACTTACCAACCACATTCGGGATACCCTACCAAACTTCAGGAACAAA CTACAGGGACAGTTGCTCTCCATAGAACATGAAGTAGAAGCCTACAAAAATTTCAAACCAGAAGACCCA ACAAGGAAGACCAAAGCATTGCTGCAGATGGTTCAGCAATTTGCTGTGGACTTTGAGAAGAGAATTGAA GGGTCAGGGGATCAAGTAGATACCCTGGAACTCTCAGGTGGTGCTAAAATCAATCGTATTTTTCATGAA CGCTTTCCTTTTGAGATAGTAAAGATGGAGTTCAATGAGAAAGAATTGCGAAGAGAAATAAGCTATGCA ATCAAAAACATACATGGTATCAGGACAGGGTTGTTTACTCCAGACATGGCATTTGAAGCGATAGTCAAG AAACAGATTGTAAAGTTGAAAGGGCCTTCCTTGAAGAGTGTGGATCTGGTAATACAAGAATTAATCAAC ACTGTGAAGAAGTGTACCAAAAAACTGGCAAACTTCCCCAGACTCTGCGAGGAAACGGAAAGGATTGTT GCTAACCACATTCGTGAGCGAGAAGGGAAGACAAAGGACCAGGTATTGCTATTGATTGACATTCAAGTC TCTTACATCAACACCAACCATGAAGACTTCATTGGCTTCGCAAATGCTCAGCAGAGGAGCAGTCAGGTT CACAAGAAAACCACAGTTGGAAATCAGGTGATTCGCAAGGGGTGGCTCACCATCAGCAACATTGGCATC ATGAAAGGCGGCTCGAAGGGATACTGGTTCGTCCTTACTGCGGAAAGCTTGTCCTGGTATAAAGATGAT GAGAACATTTAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001278252
Insert Size	1668 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001278252.1
RefSeq Size	2354 bp
RefSeq ORF	1668 bp
Locus ID	26052
UniProt ID	Q9UQ16
Protein Pathways	Endocytosis, Fc gamma R-mediated phagocytosis
MW	62.8 kDa
Gene Summary	This gene encodes a member of a family of guanosine triphosphate (GTP)-binding proteins that associate with microtubules and are involved in vesicular transport. The encoded protein functions in the development of megakaryocytes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013] Transcript Variant: This variant (3) lacks multiple 3' coding exons and contains an alternate 3' exon, resulting in a distinct 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (c) is shorter and has a distinct C-terminus, compared to isoform a.

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